Abstract:
:We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Genetic interactions led us to suggest that PRP28 is functionally associated with the U4/U5/U6 snRNP. We have now purified the PRP28 protein from S. cerevisiae and demonstrated that it is required for the first step of splicing in vitro. Interestingly, PRP28 is not a stably associated snRNP protein. Strand displacement assays indicate that PRP28 does not exhibit RNA helicase activity, suggesting that an additional factor or factors may be required for its activation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Strauss EJ,Guthrie Cdoi
10.1093/nar/22.15.3187subject
Has Abstractpub_date
1994-08-11 00:00:00pages
3187-93issue
15eissn
0305-1048issn
1362-4962journal_volume
22pub_type
杂志文章abstract::According to recent models, as yet poorly studied architectural proteins appear to be required for local regulation of enhancer-promoter interactions, as well as for global chromosome organization. Transcription factors ZIPIC, Pita and Zw5 belong to the class of chromatin insulator proteins and preferentially bind to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw371
更新日期:2016-09-06 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki478
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.23.5060
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw036
更新日期:2016-04-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.19.9267
更新日期:1988-10-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.4.1181
更新日期:1983-02-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.13.2973
更新日期:1993-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg851
更新日期:2003-11-15 00:00:00
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pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:1991-06-11 00:00:00
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1997-07-15 00:00:00
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pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00