Abstract:
:The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of the THO complex. Aly, THO and UAP56 are all required for assembly of TREX, in which Aly directly interacts with THO subunits Thoc2 and Thoc5. Both Aly and THO function in linking UAP56 to the cap-binding protein CBP80. Interestingly, association of UAP56 with the spliced mRNA, but not with the pre-mRNA, requires Aly and THO. Unexpectedly, we found that Aly and THO require each other to associate with the spliced mRNA. Consistent with these biochemical results, similar to Aly and UAP56, THO plays critical roles in mRNA export. Together, we propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chi B,Wang Q,Wu G,Tan M,Wang L,Shi M,Chang X,Cheng Hdoi
10.1093/nar/gks1188subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1294-306issue
2eissn
0305-1048issn
1362-4962pii
gks1188journal_volume
41pub_type
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