Effects of double-strand break repair proteins on vertebrate telomere structure.

abstract：:TFIIH is a multisubunit factor essential for transcription initiation and promoter escape of RNA polymerase II and for the opening of damaged DNA double strands in nucleotide excision repair (NER). In this study, we have analyzed at which step of the transcription cycle TFIIH is essential for transcription by RNA poly...

journal_title：Nucleic acids research

authors： Assfalg R,Lebedev A,Gonzalez OG,Schelling A,Koch S,Iben S

更新日期：2012-01-01 00:00:00

abstract：:The heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a multi-tasking protein that acts in the cytoplasm and nucleus. We have explored the possibility that this protein is associated with telomeres and participates in their maintenance. Rat brain hnRNP A2 was shown to have two nucleic acid binding sites. In the pr...

journal_title：Nucleic acids research

authors： Moran-Jones K,Wayman L,Kennedy DD,Reddel RR,Sara S,Snee MJ,Smith R

更新日期：2005-01-19 00:00:00

abstract：:The interactions between beef tRNATrp with avian myeloblastosis reverse transcriptase have been studied by statistical chemical modifications of phosphate (ethylnitrosourea) and cytidine (dimethyl sulfate) residues, as well as by digestion of complexed tRNA by Cobra venom nuclease and Neurospora crassa endonuclease. R...

journal_title：Nucleic acids research

authors： Garret M,Romby P,Giegé R,Litvak S

更新日期：1984-03-12 00:00:00

abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) offers a rich platform for data sharing, publishing and archiving and a globally comprehensive data set for onward use by the scientific community. With a broad scope spanning raw sequencing reads, genome assemblies and functional annotation, the resource...

journal_title：Nucleic acids research

authors： Toribio AL,Alako B,Amid C,Cerdeño-Tarrága A,Clarke L,Cleland I,Fairley S,Gibson R,Goodgame N,Ten Hoopen P,Jayathilaka S,Kay S,Leinonen R,Liu X,Martínez-Villacorta J,Pakseresht N,Rajan J,Reddy K,Rosello M,Silvester N

更新日期：2017-01-04 00:00:00

abstract：:We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiroplasma sp. strain MQ1 (M.SssI). This enzyme methylates completely and exclusively CpG sequences. The Spiroplasma gene was transcribed in E. coli using its own promoter. Translation of the entire me...

journal_title：Nucleic acids research

authors： Renbaum P,Abrahamove D,Fainsod A,Wilson GG,Rottem S,Razin A

更新日期：1990-03-11 00:00:00

abstract：:Homo-oligomerization of proteins is abundant in nature, and is often intimately related with the physiological functions of proteins, such as in metabolism, signal transduction or immunity. Information on the homo-oligomer structure is therefore important to obtain a molecular-level understanding of protein functions ...

journal_title：Nucleic acids research

authors： Baek M,Park T,Heo L,Park C,Seok C

更新日期：2017-07-03 00:00:00

abstract：:The location of proteins on the mitochondrial DNA (mtDNA) of Drosophila virilis was investigated by Me3 psoralen photoreaction of mitochondria isolated from embryos. After photoreaction the mtDNA was purified and the pattern of DNA cross-linking was determined by electron microscopy of the DNA under totally denaturing...

journal_title：Nucleic acids research

authors： Pardue ML,Fostel JM,Cech TR

更新日期：1984-02-24 00:00:00

abstract：:Sodium is one of the most abundant metals in the environment and in biology, playing critical ecological and physiological roles. Na+ is also the most common buffer salt for nucleic acids research, while its specific interaction with DNA has yet to be fully studied. Herein, we probe a highly selective and robust Na+ a...

journal_title：Nucleic acids research

authors： Zhou W,Ding J,Liu J

更新日期：2016-12-01 00:00:00

abstract：:Twenty AdoMet-dependent methyltransferases (MTases) have been characterized structurally by X-ray crystallography and NMR. These include seven DNA MTases, five RNA MTases, four protein MTases and four small molecule MTases acting on the carbon, oxygen or nitrogen atoms of their substrates. The MTases share a common co...

journal_title：Nucleic acids research

authors： Cheng X,Roberts RJ

更新日期：2001-09-15 00:00:00

abstract：:Over 50 introns have been reported in archaeal rRNA genes (rDNAs), a subset of which nests putative homing endonuclease (HEase) genes. Here, we report the identification and characterization of a novel archaeal LAGLIDADG-type HEase, I-ApeKI [corrected], encoded by the ApeK1.S908 intron within the 16S rDNA of Aeropyrum...

journal_title：Nucleic acids research

authors： Nomura N,Morinaga Y,Shirai N,Sako Y

更新日期：2005-07-26 00:00:00

abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

journal_title：Nucleic acids research

authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

更新日期：2014-04-01 00:00:00

abstract：:Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that ...

journal_title：Nucleic acids research

authors： Ndamukong I,Lapko H,Cerny RL,Avramova Z

更新日期：2011-06-01 00:00:00

abstract：:Mixed-lineage leukemia (MLL) maintains the expression of cellular memory genes during development, while leukemic MLL fusion proteins aberrantly maintain expression of hematopoietic stem cell program genes such as HOXA9 to cause leukemia. However, the molecular mechanism of gene activation is unclear. Here we show tha...

journal_title：Nucleic acids research

authors： Okuda H,Kawaguchi M,Kanai A,Matsui H,Kawamura T,Inaba T,Kitabayashi I,Yokoyama A

更新日期：2014-04-01 00:00:00

abstract：:Identification of common sub-sequences for a group of functionally related DNA sequences can shed light on the role of such elements in cell-specific gene expression. In the megakaryocytic lineage, no one single unique transcription factor was described as linage specific, raising the possibility that a cluster of gen...

journal_title：Nucleic acids research

authors： Hazony Y,Lu J,St Hilaire C,Ravid K

更新日期：2006-01-01 00:00:00

abstract：:Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated oligodeoxyribonucleotides (ODNs). Plasmid DNA was modified in vitro by ODNs containing all four DNA bases in the presence of Escherichia coli RecA protein. Up to 50% of plasmid molecules were alkylated in the targeted ...

journal_title：Nucleic acids research

authors： Singer MJ,Podyminogin MA,Metcalf MA,Reed MW,Brown DA,Gamper HB,Meyer RB,Wydro RM

更新日期：1999-12-15 00:00:00

abstract：:Poly(ADP-ribosyl)ation regulates numerous cellular processes like genome maintenance and cell death, thus providing protective functions but also contributing to several pathological conditions. Poly(ADP-ribose) (PAR) molecules exhibit a remarkable heterogeneity in chain lengths and branching frequencies, but the biol...

journal_title：Nucleic acids research

authors： Aberle L,Krüger A,Reber JM,Lippmann M,Hufnagel M,Schmalz M,Trussina IREA,Schlesiger S,Zubel T,Schütz K,Marx A,Hartwig A,Ferrando-May E,Bürkle A,Mangerich A

更新日期：2020-10-09 00:00:00

abstract：:KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately clo...

journal_title：Nucleic acids research

authors： Shafit-Zagardo B,Maio JJ,Brown FL

更新日期：1982-05-25 00:00:00

abstract：:The mutations of 76 haemophilia B patients representing the whole population registered with the Malmö haemophilia centre (42) and referrals from the UK, were characterised. RFLP haplotype analysis of the defective genes indicated that 51 single base pair substitutions were definitely of independent origin and 27 of t...

journal_title：Nucleic acids research

authors： Green PM,Montandon AJ,Bentley DR,Ljung R,Nilsson IM,Giannelli F

更新日期：1990-06-11 00:00:00

abstract：:The EMBL Data Library was the first internationally supported central resource for nucleic acid sequence data. Working in close collaboration with its American counterpart, GenBank (1), the library prepares and makes available to the scientific community a comprehensive collection of the published nucleic acid sequenc...

journal_title：Nucleic acids research

authors： Hamm GH,Cameron GN

更新日期：1986-01-10 00:00:00

abstract：:DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less...

journal_title：Nucleic acids research

authors： Yoshida S,Ungers G,Rosenberg BH

更新日期：1977-01-01 00:00:00

abstract：:CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA-aided self-cleavag...

journal_title：Nucleic acids research

authors： Shen CC,Hsu MN,Chang CW,Lin MW,Hwu JR,Tu Y,Hu YC

更新日期：2019-02-20 00:00:00

abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...

journal_title：Nucleic acids research

authors： Seurinck J,Van de Voorde A,Van Montagu M

更新日期：1983-07-11 00:00:00

abstract：:The transactivator responsive region (TAR) present in the 5'-NTR of the HIV-1 genome represents a potential target for antiretroviral intervention and a model system for the development of specific inhibitors of RNA-protein interaction. Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA(TAR)) con...

journal_title：Nucleic acids research

authors： Tripathi S,Chaubey B,Ganguly S,Harris D,Casale RA,Pandey VN

更新日期：2005-08-02 00:00:00

abstract：:The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modifi...

journal_title：Nucleic acids research

authors： Motorin Y,Lyko F,Helm M

更新日期：2010-03-01 00:00:00

abstract：:Recent advances in next-generation sequencing technologies and genome assembly algorithms have enabled the accumulation of a huge volume of genome sequences from various species. This has provided new opportunities for large-scale comparative genomics studies. Identifying and utilizing synteny blocks, which are genomi...

journal_title：Nucleic acids research

authors： Lee J,Hong WY,Cho M,Sim M,Lee D,Ko Y,Kim J

更新日期：2016-07-08 00:00:00

abstract：:We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisa...

journal_title：Nucleic acids research

authors： Walker JN,Dean PD,Saunders JR

更新日期：1986-02-11 00:00:00

abstract：:The stability of chromosome ends, the telomeres, is dependent on the ribonucleoprotein telomerase. In vitro, telomerase requires at least one RNA molecule and a reverse transcriptase-like protein. However, for telomere homeostasis in vivo, additional proteins are required. Telomerase RNAs of different species vary in ...

journal_title：Nucleic acids research

authors： Laterreur N,Eschbach SH,Lafontaine DA,Wellinger RJ

更新日期：2013-09-01 00:00:00

abstract：:Previous studies have implicated the DE-1 (-111/-106) and alpha A-CRYBP1 (-66/-57) sites for activity of the mouse alpha A-crystallin promoter in transiently transfected lens cells. Here we have used the bacterial chloramphenicol acetyltransferase (CAT) reporter gene to test the functional importance of the putative D...

journal_title：Nucleic acids research

authors： Sax CM,Ilagan JG,Piatigorsky J

更新日期：1993-06-11 00:00:00

abstract：:Spectroscopic studies of the complexes of double-stranded (ds) DNA with the polymethylene-amine linked heterodimers thiazole orange-thiazole blue, thiazole orange-ethidium, and fluorescein-ethidium, in each case show efficient energy transfer from donor to acceptor chromophores (Benson, S.C., Singh, P. and Glazer, A.N...

journal_title：Nucleic acids research

authors： Benson SC,Mathies RA,Glazer AN

更新日期：1993-12-11 00:00:00

abstract：:EcoGene (http://ecogene.org) is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

journal_title：Nucleic acids research

authors： Zhou J,Rudd KE

更新日期：2013-01-01 00:00:00