Abstract:
:The interactions between beef tRNATrp with avian myeloblastosis reverse transcriptase have been studied by statistical chemical modifications of phosphate (ethylnitrosourea) and cytidine (dimethyl sulfate) residues, as well as by digestion of complexed tRNA by Cobra venom nuclease and Neurospora crassa endonuclease. Results with nucleases and chemicals show that reverse transcriptase interacts preferentially with the D arm, the anticodon stem and the T psi stem. All these regions are located in the outside of the L-shaped structure of tRNA. This domain of interaction is different to that reported previously in the complex of beef tRNA with the cognate aminoacyl-tRNA synthetase (M. Garret et al.; Eur. J. Biochem. In press). Avian reverse transcriptase destabilizes the region of tRNA where most of the tertiary interactions maintaining the structure of tRNA are located.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Garret M,Romby P,Giegé R,Litvak Sdoi
10.1093/nar/12.5.2259subject
Has Abstractpub_date
1984-03-12 00:00:00pages
2259-71issue
5eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.2889
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa590
更新日期:2020-10-09 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.2.315
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journal_title:Nucleic acids research
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更新日期:2015-07-13 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1980-04-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.3.629
更新日期:1983-02-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv106
更新日期:2015-03-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw689
更新日期:2016-10-14 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.18.4121
更新日期:1998-09-15 00:00:00
abstract::Three clones containing both the human G gamma and A gamma globlin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clon...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1979-11-10 00:00:00
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pub_type: 杂志文章
doi:10.1093/nar/4.5.1449
更新日期:1977-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt823
更新日期:2013-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1378
更新日期:2014-04-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1986-05-27 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.12.2511
更新日期:1999-06-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-03-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq827
更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq464
更新日期:2010-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2001-10-15 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.15.2894
更新日期:1996-08-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh558
更新日期:2004-04-30 00:00:00