Abstract:
:Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that a novel member of the Trithorax family has a role unrelated to chromatin. It is encoded from an internal promoter in the ATX1 locus as an isoform containing only the SET domain (soloSET). It is located exclusively in the cytoplasm and its substrate is the elongation factor 1A (EF1A). Loss of SET, but not of the histone modifying ATX1-SET activity, affects cytoskeletal actin bundling illustrating that the two isoforms have distinct functions in Arabidopsis cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ndamukong I,Lapko H,Cerny RL,Avramova Zdoi
10.1093/nar/gkq1300subject
Has Abstractpub_date
2011-06-01 00:00:00pages
4709-18issue
11eissn
0305-1048issn
1362-4962pii
gkq1300journal_volume
39pub_type
杂志文章abstract::The distribution and amount of 5-methylcytosine (5-MeCyt) in DNA was measured for early embryos of mouse strain CF1 (2 to 4 cell stage to blastocyst) and mouse teratocarcinoma cells. In each case, the pattern of methylation was examined by use of the restriction enzymes Hha I and HPA II HPA II, which cut DNA at the si...
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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