TFIIH is an elongation factor of RNA polymerase I.

abstract：:Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...

journal_title：Nucleic acids research

authors： Damasceno JD,Obonaga R,Silva GLA,Reis-Cunha JL,Duncan SM,Bartholomeu DC,Mottram JC,McCulloch R,Tosi LRO

更新日期：2018-12-14 00:00:00

abstract：:p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...

journal_title：Nucleic acids research

authors： Al-Mohanna MA,Al-Khalaf HH,Al-Yousef N,Aboussekhra A

更新日期：2007-01-01 00:00:00

abstract：:Motif3D is a web-based protein structure viewer designed to allow sequence motifs, and in particular those contained in the fingerprints of the PRINTS database, to be visualised on three-dimensional (3D) structures. Additional functionality is provided for the rhodopsin-like G protein-coupled receptors, enabling finge...

journal_title：Nucleic acids research

authors： Gaulton A,Attwood TK

更新日期：2003-07-01 00:00:00

abstract：:Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated development of a PCR-based assay, 'telomeric repeat amplification protocol' (TRAP). Unfortunately, this method is not without dif...

journal_title：Nucleic acids research

authors： Krupp G,Kühne K,Tamm S,Klapper W,Heidorn K,Rott A,Parwaresch R

更新日期：1997-02-15 00:00:00

abstract：:The crystal structure of d(CATGGGCCCATG)(2) shows unique stacking patterns of a stable B<-->A-DNA intermediate. We evaluated intrinsic base stacking energies in this crystal structure using an ab initio quantum mechanical method. We found that all crystal base pair steps have stacking energies close to their values in...

journal_title：Nucleic acids research

authors： Poner J,Florián J,Ng HL,Poner JE,Packová N

更新日期：2000-12-15 00:00:00

abstract：:Oligoribonucleotides containing a 5'-phosphorothiolate linkage have provided effective tools to study the mechanisms of RNA catalysis, allowing resolution of kinetic ambiguity associated with mechanistic dissection and providing a strategy to establish linkage between catalysis and specific functional groups. However,...

journal_title：Nucleic acids research

authors： Li NS,Frederiksen JK,Koo SC,Lu J,Wilson TJ,Lilley DM,Piccirilli JA

更新日期：2011-03-01 00:00:00

abstract：:A cDNA library was constructed from the mRNA of the Ig lambda producing Burkitt's lymphoma cell line, EB4. Overlapping clones encompassing the coding sequence of the Ig lambda mRNA were isolated and sequenced. The predicted amino acid sequence shows a short hydrophobic leader peptide and a mature polypeptide of 217 re...

journal_title：Nucleic acids research

authors： Anderson ML,Brown L,McKenzie E,Kellow JE,Young BD

更新日期：1985-04-25 00:00:00

abstract：:The effect of the modified nucleoside pseudouridine (psi) on RNA structure was compared with uridine. The extent of base stacking in model RNA oligonucleotides was measured by 1H NMR, UV, and CD spectroscopy. The UV and CD results indicate that the model single-stranded oligoribonucleotides AAUA and AA psi A form stac...

journal_title：Nucleic acids research

authors： Davis DR

更新日期：1995-12-25 00:00:00

abstract：:The U2 snRNP complex contains two specific proteins, U2B" and U2A'. We have analysed the interaction of U2A' with U2B" and with U2 RNA. U2A' can form an weak but detectable RNA-protein complex with U2 RNA and a stable protein complex with U2B". This protein-protein complex binds efficiently and specifically to U2 RNA....

journal_title：Nucleic acids research

authors： Boelens W,Scherly D,Beijer RP,Jansen EJ,Dathan NA,Mattaj IW,van Venrooij WJ

更新日期：1991-02-11 00:00:00

abstract：:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisel...

journal_title：Nucleic acids research

authors： Jaye M,de la Salle H,Schamber F,Balland A,Kohli V,Findeli A,Tolstoshev P,Lecocq JP

更新日期：1983-04-25 00:00:00

abstract：:DNA guanine quadruplexes or G4s are non-canonical DNA secondary structures which affect genomic processes like replication, transcription and recombination. G4s are computationally identified by specific nucleotide motifs which are also called putative G4 (PG4) motifs. Despite the general relevance of these structures...

journal_title：Nucleic acids research

authors： Dhapola P,Chowdhury S

更新日期：2016-07-08 00:00:00

abstract：:The genome stability is maintained by coordinated action of DNA repairs and checkpoints, which delay progression through the cell cycle in response to DNA damage. Rad9 is conserved from yeast to human and functions in cell cycle checkpoint controls. Here, a regulatory mechanism for Rad9 function is reported. In this s...

journal_title：Nucleic acids research

authors： He W,Ma X,Yang X,Zhao Y,Qiu J,Hang H

更新日期：2011-06-01 00:00:00

abstract：:Transcription factors (TFs) are sequence-specific DNA-binding proteins acting as critical regulators of gene expression. The Plant Promoter Analysis Navigator (PlantPAN; http://PlantPAN2.itps.ncku.edu.tw) provides an informative resource for detecting transcription factor binding sites (TFBSs), corresponding TFs, and ...

journal_title：Nucleic acids research

authors： Chow CN,Zheng HQ,Wu NY,Chien CH,Huang HD,Lee TY,Chiang-Hsieh YF,Hou PF,Yang TY,Chang WC

更新日期：2016-01-04 00:00:00

abstract：:The 'Atlas of Genetics and Cytogenetics in Oncology and Haematology' (http://www.infobiogen.fr/services/chromcancer) is an Internet database aimed at genes involved in cancer, cytogenetics and clinical entities in cancer, and cancer-prone diseases. It presents information in concise and updated reviews (cards) or long...

journal_title：Nucleic acids research

authors： Huret JL,Dessen P,Bernheim A

更新日期：2001-01-01 00:00:00

abstract：:PAX6 is a transcription factor that plays a major role in ocular morphogenesis. PAX6 is expressed in the eye, central nervous system and pancreas. Two alternative promoters, P0 and P1, which are differentially regulated during development, drive PAX6 transcription. We identified a 57 bp cis-regulatory element in exon ...

journal_title：Nucleic acids research

authors： Zheng JB,Zhou YH,Maity T,Liao WS,Saunders GF

更新日期：2001-10-01 00:00:00

abstract：:To investigate the mechanism of N4-aminocytidine-induced mutagenesis, N'-alkyl-N4-aminocytidines and N4-alkyl-N4-aminocytidines were prepared and their mutagenicity on bacteria were assayed. N'-Methyl-N4-aminocytidine, N'-(2-hydroxyethyl)-N4-aminocytidine and N',N'-dimethyl-N4-aminocytidine showed direct-acting mutage...

journal_title：Nucleic acids research

authors： Nomura A,Negishi K,Hayatsu H

更新日期：1985-12-20 00:00:00

abstract：:The allele frequency net database (http://www.allelefrequencies.net) is an online repository that contains information on the frequencies of immune genes and their corresponding alleles in different populations. The extensive variability observed in genes and alleles related to the immune system response and its signi...

journal_title：Nucleic acids research

authors： Gonzalez-Galarza FF,Christmas S,Middleton D,Jones AR

更新日期：2011-01-01 00:00:00

abstract：:The HpaR-mediated regulation of the hpa-meta operon (Pg promoter) of the 4-hydroxyphenylacetic acid catabolic pathway of Escherichia coli has been studied. The HpaR regulator was purified to homogeneity showing that it is able to bind selectively to 4-hydroxyphenylacetic, 3-hydroxyphenylacetic and 3,4-dihydroxyphenyla...

journal_title：Nucleic acids research

authors： Galán B,Kolb A,Sanz JM,García JL,Prieto MA

更新日期：2003-11-15 00:00:00

abstract：:The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the lef...

journal_title：Nucleic acids research

authors： Sasaki M,Kumagai H,Takegawa K,Tohda H

更新日期：2013-05-01 00:00:00

abstract：:A synthetic gene for somatomedin C, a human growth factor, has been assembled by a single ligation of 23 oligodeoxyribonucleotides, which were chemically synthesized by an improved solid phase phosphotriester method. ...

journal_title：Nucleic acids research

authors： Sproat BS,Gait MJ

更新日期：1985-04-25 00:00:00

abstract：:TGGCA-binding proteins are nuclear proteins with high affinity for double-stranded DNA homologous to the prototype recognition sequence 5'YTGGCANNNTGCCAR 3'. Their ubiquitous tissue distribution in higher vertebrates characterizes them as a class of highly conserved proteins which may exert a basic function. To obtain...

journal_title：Nucleic acids research

authors： Nowock J,Borgmeyer U,Püschel AW,Rupp RA,Sippel AE

更新日期：1985-03-25 00:00:00

abstract：:Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...

journal_title：Nucleic acids research

authors： Ueyama M,Tori K,Ikehara M,Kaneko M

更新日期：1974-06-01 00:00:00

abstract：:Current DNA assembly methods for preparing highly purified linear subassemblies require complex and time-consuming in vitro manipulations that hinder their ability to construct megabase-sized DNAs (e.g. synthetic genomes). We have developed a new method designated 'CasHRA (Cas9-facilitated Homologous Recombination Ass...

journal_title：Nucleic acids research

authors： Zhou J,Wu R,Xue X,Qin Z

更新日期：2016-08-19 00:00:00

abstract：:Variable regions within ribosomal RNAs frequently vary in length as a result of incorporating products of slippage. This makes constructing secondary structure models problematic because base homology is difficult or impossible to establish between species. Here, we model such a region by comparing the results of the ...

journal_title：Nucleic acids research

authors： Hancock JM,Vogler AP

更新日期：1998-04-01 00:00:00

abstract：:The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...

journal_title：Nucleic acids research

authors： Li Z,Zhou M,Cai Z,Liu H,Zhong W,Hao Q,Cheng D,Hu X,Hou J,Xu P,Xue Y,Zhou Y,Xu T

更新日期：2018-12-14 00:00:00

abstract：:ArrayOme is a new program that calculates the size of genomes represented by microarray-based probes and facilitates recognition of key bacterial strains carrying large numbers of novel genes. Protein-coding sequences (CDS) that are contiguous on annotated reference templates and classified as 'Present' in the test st...

journal_title：Nucleic acids research

authors： Ou HY,Smith R,Lucchini S,Hinton J,Chaudhuri RR,Pallen M,Barer MR,Rajakumar K

更新日期：2005-01-07 00:00:00

abstract：:A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned...

journal_title：Nucleic acids research

authors： Schollmeier K,Gärtner D,Hillen W

更新日期：1985-06-25 00:00:00

abstract：:Nucleotide substitution, insertion and deletion (indel) events are the major driving forces that have shaped genomes. Using the recently identified human ribosomal protein (RP) pseudogene sequences, we have thoroughly studied DNA mutation patterns in the human genome. We analyzed a total of 1726 processed RP pseudogen...

journal_title：Nucleic acids research

authors： Zhang Z,Gerstein M

更新日期：2003-09-15 00:00:00

abstract：:Poly(ADP-ribosyl)ation is involved in numerous bio-logical processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading enzyme PAR glycohydrolase (PARG), controlling the cell fate decision between life and death in re...

journal_title：Nucleic acids research

authors： Illuzzi G,Fouquerel E,Amé JC,Noll A,Rehmet K,Nasheuer HP,Dantzer F,Schreiber V

更新日期：2014-07-01 00:00:00

abstract：:The transfer ribonucleic acids (tRNAs) of B. subtilis at different growth phases are examined for changes in the composition and the methylation of minor constituents. The composition of the tRNAs indicates about equal amounts of adenosine and uridine, and of guanosine and cytidine. About 3-4 residues are present as m...

journal_title：Nucleic acids research

authors： Singhal RP,Vold B

更新日期：1976-05-01 00:00:00