Changes in transfer ribonucleic acids of Bacillus subtilis during different growth phases.

abstract：:The effects of the treatment of cultured human and simian cells with Cadmium (Cd), a toxic and carcinogenic metal, were first assayed on macromolecular synthesis. It was observed that DNA synthesis was inhibited by Cd concentrations considerably lower than those inhibiting protein and RNA synthesis. Because of the nec...

journal_title：Nucleic acids research

authors： Nocentini S

更新日期：1987-05-26 00:00:00

abstract：:The advent of RNA-sequencing and microarray technologies has led to rapid growth of transcriptome data generated for a wide range of organisms, under various cellular, organ and individual conditions. Since the number of possible combinations of intercellular and extracellular conditions is almost unlimited, catalogin...

journal_title：Nucleic acids research

authors： Obayashi T,Kagaya Y,Aoki Y,Tadaka S,Kinoshita K

更新日期：2019-01-08 00:00:00

abstract：:The Biodegradative Strain Database (BSD) is a freely-accessible, web-based database providing detailed information on degradative bacteria and the hazardous substances that they degrade, including corresponding literature citations, relevant patents and links to additional web-based biological and chemical data. The B...

journal_title：Nucleic acids research

authors： Urbance JW,Cole J,Saxman P,Tiedje JM

更新日期：2003-01-01 00:00:00

abstract：:KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately clo...

journal_title：Nucleic acids research

authors： Shafit-Zagardo B,Maio JJ,Brown FL

更新日期：1982-05-25 00:00:00

abstract：:The 279-bp major breakpoint region (mbr) within the 3'-untranslated region (3'-UTR) of the BCL2 gene is a binding site of special AT-rich sequence binding protein 1 (SATB1) that is well known to participate in the long-range regulation of gene transcription. Our previous studies have revealed that the mbr could regula...

journal_title：Nucleic acids research

authors： Gong F,Sun L,Wang Z,Shi J,Li W,Wang S,Han X,Sun Y

更新日期：2011-06-01 00:00:00

abstract：:In order to construct fish specific expression vectors for studies on gene regulation in vitro and in vivo a variety of heterologous enhancers and promoters from mammals and from viruses of higher vertebrate cells were tested for expression of the bacterial chloramphenicol acetyl transferase reporter gene in three tel...

journal_title：Nucleic acids research

authors： Friedenreich H,Schartl M

更新日期：1990-06-11 00:00:00

abstract：:We have used in vitro site-directed mutagenesis with synthetic DNA oligonucleotides to introduce single nucleotide mutations in yeast mtDNA. In addition to the expected DNA alterations we also recovered with high frequency mutants with large deletions and insertions which arose through interaction with the synthetic D...

journal_title：Nucleic acids research

authors： Osinga KA,Van der Bliek AM,Van der Horst G,Groot Koerkamp MJ,Tabak HF,Veeneman GH,Van Boom JH

更新日期：1983-12-20 00:00:00

abstract：:Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chro...

journal_title：Nucleic acids research

authors： Kubli E,Schmidt T

更新日期：1978-05-01 00:00:00

abstract：:Genome-wide mapping in the identification of novel candidate genes has always been the standard method in genetics and genomics to correlate a clinically interesting phenotypic trait with a genotype. However, the performance of a mapping experiment using classical microsatellite approaches can be very time consuming. ...

journal_title：Nucleic acids research

authors： Klaften M,Hrabé de Angelis M

更新日期：2005-07-01 00:00:00

abstract：:Despite systematic approaches to mapping networks of genetic interactions in Saccharomyces cerevisiae, exploration of genetic interactions on a genome-wide scale has been limited. The S. cerevisiae haploid genome has 110 regions that are longer than 10 kb but harbor only non-essential genes. Here, we attempted to dele...

journal_title：Nucleic acids research

authors： Kaboli S,Yamakawa T,Sunada K,Takagaki T,Sasano Y,Sugiyama M,Kaneko Y,Harashima S

更新日期：2014-09-01 00:00:00

abstract：:The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...

journal_title：Nucleic acids research

authors： Lessard F,Stefanovsky V,Tremblay MG,Moss T

更新日期：2012-07-01 00:00:00

abstract：:Triethylammonium uridine-3',5'-cyclic phosphorothioate crystallizes in space group P2(1)2(1)2(1), a = 7.177(1), b = 13.155(6), c = 21.114(7) A, C15H26N3O7PS, MW 423.4, Z = 4, dx = 1.41g/cm3. The crystal structure was solved by direct methods on the basis of 1493 counter X-ray diffraction data (CuK alpha) and refined t...

journal_title：Nucleic acids research

authors： Hinrichs W,Steifa M,Saenger W,Eckstein F

更新日期：1987-06-25 00:00:00

abstract：:MethBank (http://bigd.big.ac.cn/methbank) is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multi...

journal_title：Nucleic acids research

authors： Li R,Liang F,Li M,Zou D,Sun S,Zhao Y,Zhao W,Bao Y,Xiao J,Zhang Z

更新日期：2018-01-04 00:00:00

abstract：:Human Ape2 protein has 3' phosphodiesterase activity for processing 3'-damaged DNA termini, 3'-5' exonuclease activity that supports removal of mismatched nucleotides from the 3'-end of DNA, and a somewhat weak AP-endonuclease activity. However, very little is known about the role of Ape2 in DNA repair processes. Here...

journal_title：Nucleic acids research

authors： Burkovics P,Hajdú I,Szukacsov V,Unk I,Haracska L

更新日期：2009-07-01 00:00:00

abstract：:Sequencing by hybridization (SBH) approaches to DNA sequencing face two conflicting constraints. First, in order to ensure that the target DNA binds reliably, the oligonucleotide probes that are attached to the chip array must be >15 bp in length. Secondly, the total number of possible 15 bp oligonucleotides is too la...

journal_title：Nucleic acids research

authors： Fedrigo O,Naylor G

更新日期：2004-02-18 00:00:00

abstract：:The Chemical Effects in Biological Systems database (CEBS) is a comprehensive and unique toxicology resource that compiles individual and summary animal data from the National Toxicology Program (NTP) testing program and other depositors into a single electronic repository. CEBS has undergone significant updates in re...

journal_title：Nucleic acids research

authors： Lea IA,Gong H,Paleja A,Rashid A,Fostel J

更新日期：2017-01-04 00:00:00

abstract：:Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B respectively. Analysis of in vitro replication products by two-dimensional agar...

journal_title：Nucleic acids research

authors： Kunnimalaiyaan M,Nielsen BL

更新日期：1997-09-15 00:00:00

abstract：:NF-IL6beta regulates gene expression and plays function roles in many tissues. The EGF-regulated cyclooxygenase-2 (cox-2) expression is mediated through p38(MAPK) signaling pathway and positively correlates with NF-IL6beta expression in A431 cells. NF-IL6beta coordinated with c-Jun on cox-2 transcriptional activation ...

journal_title：Nucleic acids research

authors： Wang JM,Ko CY,Chen LC,Wang WL,Chang WC

更新日期：2006-01-05 00:00:00

abstract：:Thousands of eukaryotic protein-coding genes generate circular RNAs that have covalently linked ends and are resistant to degradation by exonucleases. To prove their circularity as well as biochemically enrich these transcripts, it has become standard in the field to use the 3'-5' exonuclease RNase R. Here, we demonst...

journal_title：Nucleic acids research

authors： Xiao MS,Wilusz JE

更新日期：2019-09-19 00:00:00

abstract：:The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing e...

journal_title：Nucleic acids research

authors： Bengtsson M,Karlsson HJ,Westman G,Kubista M

更新日期：2003-04-15 00:00:00

abstract：:The cells of the bronchial epithelium of man are targets for benzo(a)pyrene carcinogenesis. When cultures of these cells, and of non-target fibroblasts, are exposed to [3H]-benzo(a)pyrene, we find that the epithelial cells metabolise and bind to DNA far greater amounts of benzpyrene than do fibroblasts. By analysis of...

journal_title：Nucleic acids research

authors： Arrand JE,Murray AM

更新日期：1982-03-11 00:00:00

abstract：:Interactions between proteins bound to distant sites along a DNA molecule require bending and twisting deformations in the intervening DNA. In certain systems, the sterically allowed protein-DNA and protein-protein interactions are hypothesized to produce loops with distinct geometries that may also be thermodynamical...

journal_title：Nucleic acids research

authors： Lia G,Semsey S,Lewis DE,Adhya S,Bensimon D,Dunlap D,Finzi L

更新日期：2008-07-01 00:00:00

abstract：:Aberrant DNA methylation at CpG dinucleotides can result in epigenetic silencing of tumour suppressor genes and represents one of the earliest events in tumourigenesis. To date, however, high-throughput tools that are capable of surveying the methylation status of multiple gene promoters have been restricted to a limi...

journal_title：Nucleic acids research

authors： Mund C,Beier V,Bewerunge P,Dahms M,Lyko F,Hoheisel JD

更新日期：2005-04-28 00:00:00

abstract：:Nematode mitochondria expresses two types of extremely truncated tRNAs that are specifically recognized by two distinct elongation factor Tu (EF-Tu) species named EF-Tu1 and EF-Tu2. This is unlike the canonical EF-Tu molecule that participates in the standard protein biosynthesis systems, which basically recognizes al...

journal_title：Nucleic acids research

authors： Suematsu T,Sato A,Sakurai M,Watanabe K,Ohtsuki T

更新日期：2005-08-19 00:00:00

abstract：:Increased sequencing of microbial genomes has revealed that prevailing prokaryotic species assignments can be inconsistent with whole genome information for a significant number of species. The long-standing need for a systematic and scalable species assignment technique can be met by the genome-wide Average Nucleotid...

journal_title：Nucleic acids research

authors： Varghese NJ,Mukherjee S,Ivanova N,Konstantinidis KT,Mavrommatis K,Kyrpides NC,Pati A

更新日期：2015-08-18 00:00:00

abstract：:Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present. To understand translational regulation in this system, we systematically mutated AUGs 1-4 and monitored polypeptid...

journal_title：Nucleic acids research

authors： Wise HM,Barbezange C,Jagger BW,Dalton RM,Gog JR,Curran MD,Taubenberger JK,Anderson EC,Digard P

更新日期：2011-09-01 00:00:00

abstract：:Histone proteins are synthesized in large amounts during S-phase to package the newly replicated DNA, and are among the most stable proteins in the cell. The replication-dependent (RD)-histone mRNAs expressed during S-phase end in a conserved stem-loop rather than a polyA tail. In addition, there are replication-indep...

journal_title：Nucleic acids research

authors： Lyons SM,Cunningham CH,Welch JD,Groh B,Guo AY,Wei B,Whitfield ML,Xiong Y,Marzluff WF

更新日期：2016-11-02 00:00:00

abstract：:Strategies to regulate gene function frequently use small interfering RNAs (siRNAs) that can be made from their shRNA precursors via Dicer. However, when the duplex components of these siRNA effectors are expressed from their respective coding genes, the RNA interference (RNAi) activity is much reduced. Here, we explo...

journal_title：Nucleic acids research

authors： Jin X,Sun T,Zhao C,Zheng Y,Zhang Y,Cai W,He Q,Taira K,Zhang L,Zhou D

更新日期：2012-02-01 00:00:00

abstract：:To date, an effective therapeutic treatment that confers strong attenuation toward coronaviruses (CoVs) remains elusive. Of all the potential drug targets, the helicase of CoVs is considered to be one of the most important. Here, we first present the structure of the full-length Nsp13 helicase of SARS-CoV (SARS-Nsp13)...

journal_title：Nucleic acids research

authors： Jia Z,Yan L,Ren Z,Wu L,Wang J,Guo J,Zheng L,Ming Z,Zhang L,Lou Z,Rao Z

更新日期：2019-07-09 00:00:00

abstract：:The MalaCards human disease database (http://www.malacards.org/) is an integrated compendium of annotated diseases mined from 68 data sources. MalaCards has a web card for each of ∼20 000 disease entries, in six global categories. It portrays a broad array of annotation topics in 15 sections, including Summaries, Symp...

journal_title：Nucleic acids research

authors： Rappaport N,Twik M,Plaschkes I,Nudel R,Iny Stein T,Levitt J,Gershoni M,Morrey CP,Safran M,Lancet D

更新日期：2017-01-04 00:00:00