Benzpyrene groups bind preferentially to the DNA of active chromatin in human lung cells.

Abstract:

:The cells of the bronchial epithelium of man are targets for benzo(a)pyrene carcinogenesis. When cultures of these cells, and of non-target fibroblasts, are exposed to [3H]-benzo(a)pyrene, we find that the epithelial cells metabolise and bind to DNA far greater amounts of benzpyrene than do fibroblasts. By analysis of nuclei of benzpyrene-treated cells for sensitivity to limited digestion with pancreatic DNase I, we have shown that benzpyrene groups bind initially to the DNA of expressed (DNase I sensitive) regions of chromatin in both cell types. Covalent binding of benzpyrene groups to non-expressed (DNase I resistant) regions follows rapidly in the target epithelial cells. These maintain high levels of carcinogen adducts in their DNA. In fibroblasts, benzpyrene group binding to non-expressed DNA occurs more slowly and active removal of adducts from the DNA is evident.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Arrand JE,Murray AM

doi

10.1093/nar/10.5.1547

subject

Has Abstract

pub_date

1982-03-11 00:00:00

pages

1547-55

issue

5

eissn

0305-1048

issn

1362-4962

journal_volume

10

pub_type

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