Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells.

abstract：:Aging research is a multi-disciplinary field encompassing knowledge from many areas of basic, applied and clinical research. Age-related processes occur on molecular, cellular, tissue, organ, system, organismal and even psychological levels, trigger the onset of multiple debilitating diseases and lead to a loss of fun...

journal_title：Nucleic acids research

authors： Moskalev A,Zhikrivetskaya S,Shaposhnikov M,Dobrovolskaya E,Gurinovich R,Kuryan O,Pashuk A,Jellen LC,Aliper A,Peregudov A,Zhavoronkov A

更新日期：2016-01-04 00:00:00

abstract：:The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130,000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77,000 Thalassiosira pseudonana ESTs from seven libraries...

journal_title：Nucleic acids research

authors： Maheswari U,Mock T,Armbrust EV,Bowler C

更新日期：2009-01-01 00:00:00

abstract：:DNA guanine quadruplexes or G4s are non-canonical DNA secondary structures which affect genomic processes like replication, transcription and recombination. G4s are computationally identified by specific nucleotide motifs which are also called putative G4 (PG4) motifs. Despite the general relevance of these structures...

journal_title：Nucleic acids research

authors： Dhapola P,Chowdhury S

更新日期：2016-07-08 00:00:00

abstract：:Initiation of Adenovirus (Ad) DNA replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (pTP) and DNA polymerase (pol) as well as two nuclear DNA-binding proteins from uninfected HeLa cells are required. Biochemical studies on the pTP and DNA polymerase proteins separately hav...

journal_title：Nucleic acids research

authors： Stunnenberg HG,Lange H,Philipson L,van Miltenburg RT,van der Vliet PC

更新日期：1988-03-25 00:00:00

abstract：:HOX11 is a homeobox-containing oncogene of specific T-cell leukemias. We determined the DNA binding specificity of the Hox11 protein by using a novel technique of random oligonucleotide selection developed in this study. The optimal Hox11 binding sequence, GGCGGTAAGTGG, contained a core TAAGTG motif that is consistent...

journal_title：Nucleic acids research

authors： Tang S,Breitman ML

更新日期：1995-06-11 00:00:00

abstract：:In the battle against viruses, antiviral peptides (AVPs) had demonstrated the immense potential. Presently, more than 15 peptide-based drugs are in various stages of clinical trials. Emerging and re-emerging viruses further emphasize the efforts to accelerate antiviral drug discovery efforts. Despite, huge importance ...

journal_title：Nucleic acids research

authors： Thakur N,Qureshi A,Kumar M

更新日期：2012-07-01 00:00:00

abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...

journal_title：Nucleic acids research

authors： Nagashima T,Matsuda H,Silva DG,Petrovsky N,Konagaya A,Schönbach C,Kasukawa T,Arakawa T,Carninci P,Kawai J,Hayashizaki Y,RIKEN GER Group.,Genome Science Laoratory Members.

更新日期：2004-01-01 00:00:00

abstract：:We present a tool for the prediction of mRNA 3'-processing (cleavage and polyadenylation) sites in the yeast Saccharomyces cerevisiae, based on a discrete state-space model or hidden Markov model. Comparison of predicted sites with experimentally verified 3'-processing sites indicates good agreement. All predicted or ...

journal_title：Nucleic acids research

authors： Graber JH,McAllister GD,Smith TF

更新日期：2002-04-15 00:00:00

abstract：:The basal transcription factor TFE enhances transcription initiation by catalysing DNA strand-separation, a process that varies with temperature and ionic strength. Canonical TFE forms a heterodimeric complex whose integrity and function critically relies on a cubane iron-sulphur cluster residing in the TFEβ subunit. ...

journal_title：Nucleic acids research

authors： Blombach F,Ausiannikava D,Figueiredo AM,Soloviev Z,Prentice T,Zhang M,Zhou N,Thalassinos K,Allers T,Werner F

更新日期：2018-03-16 00:00:00

abstract：:The interaction of N--Acetyl--Phe--tRNA Phe with 70 S ribosomes is a reversible process in the absence as well as in the presence of messenger. The equilibrium binding constants of these interactions were measured at different magnesium concentrations and temperatures and thermodynamical quantities computed. The entha...

journal_title：Nucleic acids research

authors： Odinzov VB,Kirillov SV

更新日期：1978-10-01 00:00:00

abstract：:A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants were characterized. All displayed wild-...

journal_title：Nucleic acids research

authors： Swimmer C,Shenk T

更新日期：1985-11-25 00:00:00

abstract：:A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removin...

journal_title：Nucleic acids research

authors： Kalmár T,Bachrati CZ,Marcsik A,Raskó I

更新日期：2000-06-15 00:00:00

abstract：:A linear DNA plasmid, designated pLLE1, has been isolated from a mitochondrial fraction of a strain of Lentinus edodes. pLLE1(11.0 kbp) was sensitive to the 3'----5'-acting exonuclease III and resistant to the 5'----3'-acting lambda exonuclease. It showed no homology with mitochondrial and nuclear genomic DNAs of plas...

journal_title：Nucleic acids research

authors： Katayose Y,Kajiwara S,Shishido K

更新日期：1990-03-25 00:00:00

abstract：:Generally, the presence of a consensus sequence in the promoter of a gene is taken as indication for regulation by the transcription factor that binds to this sequence. In light of the recent developments in genome research, we were interested to what extent this supposition is valid. We examined the relationship betw...

journal_title：Nucleic acids research

authors： Hamoen LW,Smits WK,de Jong A,Holsappel S,Kuipers OP

更新日期：2002-12-15 00:00:00

abstract：:Inhibition of an RNA processing reaction after treatment with the Ca2(+)-dependent micrococcal nuclease (MN) is often used as a criterion for the presence of a required RNA or ribonucleoprotein component in the system. Following MN digestion, the nuclease is inactivated with EGTA and radiolabeled substrate is added to...

journal_title：Nucleic acids research

authors： Wang MJ,Gegenheimer P

更新日期：1990-11-25 00:00:00

abstract：:Bacterial RNase III plays important roles in the processing and degradation of RNA transcripts. A major goal is to identify the cleavage targets of this endoribonuclease at a transcriptome-wide scale and delineate its in vivo cleavage rules. Here we applied to Escherichia coli grown to either exponential or stationary...

journal_title：Nucleic acids research

authors： Altuvia Y,Bar A,Reiss N,Karavani E,Argaman L,Margalit H

更新日期：2018-11-02 00:00:00

abstract：:A dinucleoside monophosphate, 6,2'-anhydro-6-oxy-1-beta-D-arabinofuranosyluracil-phosphoryl- (3'-5')-6,2'-anhydro-6-oxy-1-beta-D-arabinofuranosyluracil (I) was synthesized by the condensation reaction using DCC from 5'-monomethoxytrityl derivative(VII) and 3'-acetyl-5'-phosphate(X) of the monomer units. Yield was ca. ...

journal_title：Nucleic acids research

authors： Ikehara M,Tezuka T

更新日期：1974-03-01 00:00:00

abstract：:Mutator-like transposable elements (MULEs) are widespread across fungal, plant and animal species. Despite their abundance and importance as genetic tools in plants, the transposition mechanism of the MULE superfamily was previously unknown. Discovery of the Muta1 element from Aedes aegypti and its successful transpos...

journal_title：Nucleic acids research

authors： Liu K,Wessler SR

更新日期：2017-06-20 00:00:00

abstract：:The mouse c-Ki-ras protooncogene promoter contains an unusual DNA element consisting of a 27 bp-long homopurine-homopyrimidine mirror repeat (H-motif) adjacent to a d(C-G)5 repeat. We have previously shown that in vitro these repeats may adopt H and Z conformations, respectively, causing nuclease and chemical hypersen...

journal_title：Nucleic acids research

authors： Raghu G,Tevosian S,Anant S,Subramanian KN,George DL,Mirkin SM

更新日期：1994-08-25 00:00:00

abstract：:DNA repair is required to maintain genome stability in stem cells and early embryos. At critical junctures, oxidative damage to DNA requires the base excision repair (BER) pathway. Since early zebrafish embryos lack the major polymerase in BER, DNA polymerase ß, repair proceeds via replicative polymerases, even though...

journal_title：Nucleic acids research

authors： Pei DS,Yang XJ,Liu W,Guikema JE,Schrader CE,Strauss PR

更新日期：2011-04-01 00:00:00

abstract：:To address how eukaryotic replication forks respond to fork stalling caused by strong non-covalent protein-DNA barriers, we engineered the controllable Fob-block system in Saccharomyces cerevisiae. This system allows us to strongly induce and control replication fork barriers (RFB) at their natural location within the...

journal_title：Nucleic acids research

authors： Bentsen IB,Nielsen I,Lisby M,Nielsen HB,Gupta SS,Mundbjerg K,Andersen AH,Bjergbaek L

更新日期：2013-03-01 00:00:00

abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...

journal_title：Nucleic acids research

authors： Ohta S,Maruyama S,Nitta K,Sugai S

更新日期：1983-05-25 00:00:00

abstract：:Mitochondrial DNA (mtDNA) resides in a high ROS environment and suffers more mutations than its nuclear counterpart. Increasing evidence suggests that mtDNA mutations are not the results of direct oxidative damage, rather are caused, at least in part, by DNA replication errors. To understand how the mtDNA replicase, P...

journal_title：Nucleic acids research

authors： Anderson AP,Luo X,Russell W,Yin YW

更新日期：2020-01-24 00:00:00

abstract：:A novel method combining elements of suppression subtractive hybridization with high throughput differential screening permits the efficient and rapid cloning of rarely transcribed differentially expressed genes. The experimental strategy virtually excludes the possibility of isolating false positive clones. The poten...

journal_title：Nucleic acids research

authors： von Stein OD,Thies WG,Hofmann M

更新日期：1997-07-01 00:00:00

abstract：:Human telomerase is a ribonucleoprotein which uses its internal RNA moiety as a template for telomeric DNA synthesis. This enzyme is up-regulated in most malignant tumors and is therefore considered as a possible cancer target. Here we examined the effects of differently modified oligomers on telomeraseactivity from H...

journal_title：Nucleic acids research

authors： Matthes E,Lehmann C

更新日期：1999-02-15 00:00:00

abstract：:The first step in DNA cleavage at V(D)J recombination signals by RAG1 and RAG2 is creation of a nick at the heptamer/coding flank border. Under proper conditions in vitro the second step, hairpin formation, requires two signals with spacers of 12 and 23 bp, a restriction referred to as the 12/23 rule. Under these cond...

journal_title：Nucleic acids research

authors： Eastman QM,Schatz DG

更新日期：1997-11-01 00:00:00

abstract：:The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...

journal_title：Nucleic acids research

authors： Oda H,Taketomi A,Maruyama R,Itoh R,Nishioka K,Yakushiji H,Suzuki T,Sekiguchi M,Nakabeppu Y

更新日期：1999-11-15 00:00:00

abstract：:We present a general method for isolating molecular markers specific to any region of a chromosome using existing mapping populations. Two pools of DNA from individuals homozygous for opposing alleles for a targeted chromosomal interval, defined by two or more linked RFLP markers, are constructed from members of an ex...

journal_title：Nucleic acids research

authors： Giovannoni JJ,Wing RA,Ganal MW,Tanksley SD

更新日期：1991-12-11 00:00:00

abstract：:We have analyzed the molecular mechanism that makes translation of the MS2 replicase cistron dependent on the translation of the upstream coat cistron. Deletion mapping on cloned cDNA of the phage shows that the ribosomal binding site of the replicase cistron is masked by a long distance basepairing to an internal coa...

journal_title：Nucleic acids research

authors： Berkhout B,van Duin J

更新日期：1985-10-11 00:00:00

abstract：:In some preparations of DNA dependent RNA polymerase a new enzymatic activity has been found which catalyzes the condensation of two pyrophosphate molecules, liberated in the process of RNA synthesis, to one molecule of orthophosphate and one molecule of Mg (or Mn) - chelate complex with trimetaphosphate. This activit...

journal_title：Nucleic acids research

authors： Volloch VZ,Rits S,Tumerman L

更新日期：1979-04-01 00:00:00