Abstract:
:In some preparations of DNA dependent RNA polymerase a new enzymatic activity has been found which catalyzes the condensation of two pyrophosphate molecules, liberated in the process of RNA synthesis, to one molecule of orthophosphate and one molecule of Mg (or Mn) - chelate complex with trimetaphosphate. This activity can also cooperate with DNA-polymerase, on condition that both enzymes originate from the same cells. These results point to two general conclusions. First, energy is conserved in the overall process of nucleic acid synthesis and turnover, so that the process does not require an energy influx from the cell's general resources. Second, the synthesis of nucleic acids is catalyzed by a complex enzyme system which contains at least two separate enzymes, one responsible for nucleic acid polymerization and the other for energy conservation via pyrophosphate condensation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Volloch VZ,Rits S,Tumerman Ldoi
10.1093/nar/6.4.1521subject
Has Abstractpub_date
1979-04-01 00:00:00pages
1521-34issue
4eissn
0305-1048issn
1362-4962journal_volume
6pub_type
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