Abstract：
:Heptadecaoligodeoxyribonucleotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these basemodified oligomers with P/A, P/G, K/C and K/T; P/A, P/G, I/C, I/T and I/A, I/G, I/C, I/T base pairs were compared by measuring their melting transition (Tm) values. Oligomers containing both P and K and P and I were more stable than those with I alone or with mismatches. These oligomers together with one with a P base at the 3'-end were used as primers in polymerase chain reaction (PCR) experiments. They were all effective primers except one with I alone and a triple mismatch. Thus the use of the degenerate bases P and K in primer design is established.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lin PK,Brown DMdoi
10.1093/nar/20.19.5149keywords:
subject
Has Abstractpub_date
1992-10-11 00:00:00pages
5149-52issue
19eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract：:Complementary DNA clones encoding the human kidney epidermal growth factor (EGF) precursor have been isolated and sequenced. They predict the sequence of a 1,207 amino acid protein which contains EGF flanked by polypeptide segments of 970 and 184 residues at its NH2- and COOH-termini, respectively. The structural orga...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.21.8427
更新日期：1986-11-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.19.3937
更新日期：1997-10-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1133
更新日期：2015-12-02 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.13.3029
更新日期：1981-07-10 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2002-06-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1081
更新日期：2011-04-01 00:00:00
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更新日期：2012-12-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr726
更新日期：2012-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw330
更新日期：2016-07-08 00:00:00
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pub_type： 杂志文章
doi：10.1093/nar/12.1part1.101
更新日期：1984-01-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw935
更新日期：2017-01-04 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.24.7013
更新日期：1981-12-21 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.2.689
更新日期：1979-02-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1165
更新日期：2019-02-20 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg626
更新日期：2003-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz815
更新日期：2019-11-18 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr792
更新日期：2012-02-01 00:00:00
abstract：:Base excision repair (BER), which is initiated by DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. The NTHL1 glycosylase, which excises DNA base damage caused by reactive oxygen species, is thought to be a tumor suppressor. However, in addition to NTHL1 loss-of-function...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky162
更新日期：2018-05-18 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.4.1287
更新日期：1979-04-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.9.3439
更新日期：1978-09-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.8.2747
更新日期：1977-08-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm453
更新日期：2007-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1151
更新日期：1976-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.16.3327
更新日期：1995-08-25 00:00:00
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pub_type： 杂志文章
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更新日期：1993-03-25 00:00:00
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pub_type： 杂志文章
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更新日期：2016-07-08 00:00:00
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pub_type： 杂志文章
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更新日期：2017-10-13 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt051
更新日期：2013-03-01 00:00:00
