Abstract：
:The DSSP program automatically assigns the secondary structure for each residue from the three-dimensional co-ordinates of a protein structure to one of eight states. However, discrete assignments are incomplete in that they cannot capture the continuum of thermal fluctuations. Therefore, DSSPcont (http://cubic.bioc.columbia.edu/services/DSSPcont) introduces a continuous assignment of secondary structure that replaces 'static' by 'dynamic' states. Technically, the continuum results from calculating weighted averages over 10 discrete DSSP assignments with different hydrogen bond thresholds. A DSSPcont assignment for a particular residue is a percentage likelihood of eight secondary structure states, derived from a weighted average of the ten DSSP assignments. The continuous assignments have two important features: (i) they reflect the structural variations due to thermal fluctuations as detected by NMR spectroscopy; and (ii) they reproduce the structural variation between many NMR models from one single model. Therefore, functionally important variation can be extracted from a single X-ray structure using the continuous assignment procedure.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Carter P,Andersen CA,Rost Bdoi
10.1093/nar/gkg626keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
3293-5issue
13eissn
0305-1048issn
1362-4962journal_volume
31pub_type
杂志文章abstract：:Tumor formation is partially driven by DNA copy number changes, which are typically measured using array comparative genomic hybridization, SNP arrays and DNA sequencing platforms. Many techniques are available for detecting recurring aberrations across multiple tumor samples, including CMAR, STAC, GISTIC and KC-SMART...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt155
更新日期：2013-05-01 00:00:00
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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doi：10.1093/nar/gkg714
更新日期：2003-09-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2008-07-01 00:00:00
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更新日期：1992-08-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1999-07-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1985-01-11 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/28.18.3433
更新日期：2000-09-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：1996-04-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh585
更新日期：2004-05-20 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
abstract：:Synthetic RNA formulations and viral vectors are the two main approaches for delivering small therapeutic RNA to human cells. Here we report findings supporting an alternative strategy in which an endogenous human RNA polymerase (RNAP) is harnessed to make RNA hairpin-containing small RNA from synthetic single-strande...
journal_title：Nucleic acids research
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更新日期：2013-02-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：1985-02-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1996-03-15 00:00:00
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更新日期：1990-01-11 00:00:00
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更新日期：1991-06-11 00:00:00
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journal_title：Nucleic acids research
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更新日期：1982-02-25 00:00:00
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更新日期：1975-05-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2006-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2015-10-15 00:00:00
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更新日期：2010-04-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：1998-06-01 00:00:00
abstract：:In fission yeast and other eukaryotes, Rec12 (Spo11) is thought to catalyze the formation of dsDNA breaks (DSBs) that initiate homologous recombination in meiosis. Rec12 is orthologous to the catalytic subunit of topoisomerase VI (Top6A). Guided by the crystal structure of Top6A, we engineered the rec12 locus to encod...
journal_title：Nucleic acids research
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更新日期：2011-03-01 00:00:00
