Abstract：
:Splicing of mammalian introns requires that the intron possess at least 80 nucleotides. This length requirement presumably reflects the constraints of accommodating multiple snRNPs simultaneously in the same intron. In the free-living nematode, C. elegans, introns typically are 45 to 55 nucleotides in length. In this report, we determine whether C. elegans introns can obviate the mammalian length requirement by virtue of their structure or sequence. We demonstrate that a 53 nucleotide intron from the unc-54 gene of C. elegans does not undergo splicing in a mammalian (HeLa) nuclear extract. However, insertion of 31 nucleotides of foreign, prokaryotic sequence into the same intron results in efficient splicing. The observed splicing proceeds by the same two-step mechanism observed with mammalian introns, and exploits the same 3' and 5' splice sites as are used in C. elegans. The branch point used lies in the inserted sequence. We conclude that C. elegans splicing components are either fewer in number or smaller than their mammalian counterparts.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ogg SC,Anderson P,Wickens MPdoi
10.1093/nar/18.1.143subject
Has Abstractpub_date
1990-01-11 00:00:00pages
143-9issue
1eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract：:Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr697
更新日期：2012-01-01 00:00:00
abstract：:The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nucl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.1.219
更新日期：1979-01-01 00:00:00
abstract：:Signaling pathways constitute a valuable source of information that allows interpreting the way in which alterations in gene activities affect to particular cell functionalities. There are web tools available that allow viewing and editing pathways, as well as representing experimental data on them. However, few metho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt451
更新日期：2013-07-01 00:00:00
abstract：:Double-strand breaks (DSBs) are dangerous chromosomal lesions that must be efficiently repaired in order to avoid loss of genetic information or cell death. In all organisms studied to date, two different mechanisms are used to repair DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Previous ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm521
更新日期：2007-01-01 00:00:00
abstract：:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, h...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp050
更新日期：2009-04-01 00:00:00
abstract：:Abasic substitutions in the non-template strand and promoter sequence changes were made to assess the roles of various promoter features in sigma70 holoenzyme interactions with fork junction probes. Removal of -10 element non-template single strand bases, leaving the phosphodiester backbone intact, did not interfere w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg400
更新日期：2003-06-01 00:00:00
abstract：:Nucleic acid double helices are stabilized by hydrogen bonding and stacking forces (a combination of hydrophobic, dispersive and electrostatic forces) of the base pairs in the helix. One would predict the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm210
更新日期：2007-01-01 00:00:00
abstract：:The initiation region of the MS2 replicase cistron can be isolated as a fragment 59 bases in length protected from RNAase by the binding of the coat protein which serves as a translational repressor. This fragment MS2 R(-53 leads to 6) starts 53 bases before the initiation codon and retains full activity in binding ri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.5.1761
更新日期：1979-01-01 00:00:00
abstract：:Stacking self-association equilibria in aqueous solutions of m3uridine, m34,2',3',5'uridine, 2'-deoxyuridine, m13,4,4cytosine, m14,4,4,5cytosine, s2cytidine and s4thymidine were studied at various temperatures by vapour-pressure osmometry. Equilibrium constants Kst's were computed on the assumption of the isodesmic mo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1295
更新日期：1976-05-01 00:00:00
abstract：:Repression of cellular reprogramming in germ cells is critical to maintaining cell fate and fertility. When germ cells mis-express somatic genes they can be directly converted into other cell types, resulting in loss of totipotency and reproductive potential. Identifying the molecular mechanisms that coordinate these ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa174
更新日期：2020-05-07 00:00:00
abstract：:Melanotransferrin (MTf) is a tumor associated antigen found in abundance on the surface of melanoma cells. It is a transferrin-like protein found in low amount in most adult tissues and whose gene is reminiscent of house-keeping genes. With the goal of understanding the regulatory mechanisms which might explain the en...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.11.2853
更新日期：1992-06-11 00:00:00
abstract：:It has been inferred from DNA sequence analyses that in echinoderm mitochondria not only the usual asparagine codons AAU and AAC, but also the usual lysine codon AAA, are translated as asparagine by a single mitochondrial (mt) tRNAAsn with the anticodon GUU. Nucleotide sequencing of starfish mt tRNAAsn revealed that t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.7.1683
更新日期：1999-04-01 00:00:00
abstract：:SSCP and heteroduplex analysis (HA) continue to be the most popular methods of mutation detection due to their simplicity, high sensitivity and low cost. The advantages of these methods are most clearly visible when large genes, such as BRCA1 and BRCA2, are scanned for scattered unknown mutations and/or when a large n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.14.e71
更新日期：2001-07-15 00:00:00
abstract：:Single-nucleotide polymorphisms (SNPs) are often linked to critical phenotypes such as diseases or responses to vaccines, medications and environmental factors. However, the specific molecular mechanisms by which a causal SNP acts is usually not obvious. Changes in RNA secondary structure emerge as a possible explanat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1009
更新日期：2013-01-07 00:00:00
abstract：:The 'regions' method for multisequence alignment used in the previously reported program MALIGN has been generalized to include recursive refinement so that unaligned portions between two regions at the current level of resolution can be handled with increased resolution. Additionally, there is incorporated a limiting...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.5.1683
更新日期：1988-03-11 00:00:00
abstract：:A DNA fragment d(GCGAAAGCT), known to adopt a stable mini-hairpin structure in solution, has been crystallized in the space group I4(1)22 with the unit-cell dimensions a = b = 53.4 A and c = 54.0 A, and the crystal structure has been determined at 2.5 A resolution. The four nucleotide residues CGAA of the first half o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf639
更新日期：2002-12-01 00:00:00
abstract：:We report a novel system for efficient and specific targeted delivery of large nucleic acids to and into cells. Plasmid DNA and core histones were assembled to chromatin by salt gradient dialysis and subsequently connected to bispecific antibody derivatives (bsAbs) via a nucleic acid binding peptide bridge. The result...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz137
更新日期：2019-06-04 00:00:00
abstract：:In order to investigate the possible role of RNA secondary structure in determining the efficiency and specificity of mRNA splicing, the structures of sequences at three acceptor splice sites in adenovirus were studied. Transcripts spanning intron-exon junctions were synthesized using SP6 RNA polymerase and analyzed u...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.22.8437
更新日期：1984-11-26 00:00:00
abstract：:The nucleotide sequence of the 5S ribosomal RNA of Streptococcus cremoris has been determined. The sequence is 5' (sequence in text) 3'. Comparison of the S. cremoris 5S RNA sequence to an updated prokaryotic generalized 5S RNA structural model shows that this 5S RNA contains some unusual structural features. These fe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.21.7569
更新日期：1983-11-11 00:00:00
abstract：:Interactions between subunits in the Saccharomyces cerevisiae ribosome are mediated by universal and eukaryote-specific intersubunit bridges. Universal bridges are positioned close to the ribosomal functional centers, while eukaryote-specific bridges are mainly located on the periphery of the ribosome. Two bridges, eB...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1083
更新日期：2019-01-10 00:00:00
abstract：:Minigenes encoding the peptide Met-Arg-Arg have been used to study the mechanism of toxicity of AGA codons proximal to the start codon or prior to the termination codon in bacteria. The codon sequences of the 'mini-ORFs' employed were initiator, combinations of AGA and CGA, and terminator. Both, AGA and CGA are low-us...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh784
更新日期：2004-08-18 00:00:00
abstract：:Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replication, but clinically useful inhibitors have not been developed. Here, we report an assay for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput screening and mechanistic studies. Initia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks325
更新日期：2012-09-01 00:00:00
abstract：:The glycoprotein C (gC) gene of herpes simplex virus type 1 is a true late gene, in that its expression occurs late in infection with a strict requirement for viral DNA replication. Recently, we reported on gC expression during infection with mutant viruses carrying deletions in the gC gene promoter. Analysis of RNA e...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.7.3097
更新日期：1987-04-10 00:00:00
abstract：:We have assessed the ability of nucleosomes to influence the formation of mammalian topoisomerase II-DNA complexes by mapping the sites of cleavage induced by four unrelated topoisomerase II inhibitors in naked versus nucleosome-reconstituted SV40 DNA. DNA fragments were reconstituted with histone octamers from HeLa c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.15.4553
更新日期：1990-08-11 00:00:00
abstract：:To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and micro-sequen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.15.2882
更新日期：2000-08-01 00:00:00
abstract：:A minor tRNA-Glu1 constituent of a preparation of E. coli B tRNA-Glu (Oak Ridge) has a guanosine residue at position 66 rather than an adenosine as in the tRNA-Glu2 described by Ohashi et al. (3). Automated sequential degradation was used to sequence this region. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.4.469
更新日期：1975-04-01 00:00:00
abstract：:The availability of the complete sequence of the Saccharomyces cerevisiae genome has allowed a comprehensive analysis of the genes encoding cytoplasmic ribosomal proteins in this organism. On the basis of this complete inventory a new nomenclature for the yeast ribosomal proteins is presented. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.24.4872
更新日期：1997-12-15 00:00:00
abstract：:Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.18.3745
更新日期：1997-09-15 00:00:00
abstract：:Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.766
更新日期：2002-02-01 00:00:00
abstract：:The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1116
更新日期：2008-03-01 00:00:00
