Abstract:
:Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tucker KL,Wang Y,Dausman J,Jaenisch Rdoi
10.1093/nar/25.18.3745subject
Has Abstractpub_date
1997-09-15 00:00:00pages
3745-6issue
18eissn
0305-1048issn
1362-4962pii
gka602journal_volume
25pub_type
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