Abstract:
:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, hF1gamma intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1gamma exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Fukumura K,Taniguchi I,Sakamoto H,Ohno M,Inoue Kdoi
10.1093/nar/gkp050subject
Has Abstractpub_date
2009-04-01 00:00:00pages
1907-14issue
6eissn
0305-1048issn
1362-4962pii
gkp050journal_volume
37pub_type
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