Abstract:
:The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to the inducer IPTG. One strong binding site in the I gene DNA was found to be one of two expected on the basis of their homology with the lac operator. The binding of I12-X86 repressor to this site was visualized using the footprinting technique, and found to be consistent with an operator-like binding configuration. The protection pattern extends into an adjacent sequence suggesting that two repressor tetramers are bound in tandem.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schmitz A,Galas DJdoi
10.1093/nar/8.3.487subject
Has Abstractpub_date
1980-02-11 00:00:00pages
487-506issue
3eissn
0305-1048issn
1362-4962journal_volume
8pub_type
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