Abstract:
:The repetitive zinc finger domain of transcription factor IIIA binds 5S DNA and 5S RNA with similar affinity. Site directed mutagenesis of the Xenopus borealis somatic 5S RNA gene has been used to produce a series of derivatives of 5S RNA containing local sequence substitutions or sequence deletions. Gel mobility shift analyses of the binding of TFIIIA to these altered 5S RNAs revealed that all three of the helical stems of the 5S RNA secondary structure are required for binding. TFIIIA was observed to bind with normal affinity to RNAs lacking 12 nucleotides at either the loop c or loop e/helix V regions of 5S RNA, as well as to a double mutant containing both deletions. The secondary structure of the resulting 96-nucleotide RNA, studied using structure-specific ribonucleases, was found to resemble the central portion of 5S RNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bogenhagen DF,Sands MSdoi
10.1093/nar/20.11.2639keywords:
subject
Has Abstractpub_date
1992-06-11 00:00:00pages
2639-45issue
11eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract::We define phosphovariants as genetic variations that change phosphorylation sites or their interacting kinases. Considering the essential role of phosphorylation in protein functions, it is highly likely that phosphovariants change protein functions. Therefore, a comparison of phosphovariants between individuals or be...
journal_title:Nucleic acids research
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更新日期:2004-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2008-04-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2018-09-06 00:00:00
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更新日期:2006-01-01 00:00:00
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更新日期:1992-04-25 00:00:00
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更新日期:2011-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1991-10-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2004-04-28 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-04-01 00:00:00
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更新日期:2017-12-15 00:00:00
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