Abstract：
:We have prepared several novel phosphoramidites and have synthesised oligonucleotides incorporating them internally. The presence of these residues in an oligonucleotide template presents an impossible barrier to primed synthesis by Taq DNA polymerase. When extended as polymerase chain reaction products, these oligonucleotides no longer serve as templates for the polymerase beyond the insertion sites of the modified intermediates, thereby producing single-stranded tails on amplification products. These tails can then be used for solid phase capture and colorimetric detection of PCR products.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Newton CR,Holland D,Heptinstall LE,Hodgson I,Edge MD,Markham AF,McLean MJdoi
10.1093/nar/21.5.1155subject
Has Abstractpub_date
1993-03-11 00:00:00pages
1155-62issue
5eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract：:Pds5 is required for sister chromatid cohesion, and somewhat paradoxically, to remove cohesin from chromosomes. We found that Pds5 plays a critical role during DNA replication that is distinct from its previously known functions. Loss of Pds5 hinders replication fork progression in unperturbed human and mouse cells. I...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky519
更新日期：2019-02-20 00:00:00
abstract：:The interactions between beef tRNATrp with avian myeloblastosis reverse transcriptase have been studied by statistical chemical modifications of phosphate (ethylnitrosourea) and cytidine (dimethyl sulfate) residues, as well as by digestion of complexed tRNA by Cobra venom nuclease and Neurospora crassa endonuclease. R...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.5.2259
更新日期：1984-03-12 00:00:00
abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that attenuate expression of their mRNA targets. Here, we developed a new method and an R package, to easily infer candidate miRNA-mRNA target interactions that could be functional during a given biological process. Using this method, we described, for the first time, a comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx327
更新日期：2017-06-02 00:00:00
abstract：:The ribosomal proteins L4 and L22 form part of the peptide exit tunnel in the large ribosomal subunit. In Escherichia coli, alterations in either of these proteins can confer resistance to the macrolide antibiotic, erythromycin. The structures of the 30S as well as the 50S subunits from each antibiotic resistant mutan...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh913
更新日期：2004-10-27 00:00:00
abstract：:New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports. No modifications to phosphor...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh222
更新日期：2004-01-29 00:00:00
abstract：:Membraneless organelles (MOs) are dynamic liquid condensates that host a variety of specific cellular processes, such as ribosome biogenesis or RNA degradation. MOs form through liquid-liquid phase separation (LLPS), a process that relies on multivalent weak interactions of the constituent proteins and other macromole...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz848
更新日期：2020-01-08 00:00:00
abstract：:Transcription start and termination sites were mapped in the E. coli replication origin, oriC. Outward transcription from within oriC (promoters Pori-r and Pori-l) was found to start in vivo at position 178 for Pori-l and at positions 294 and 304 for Pori-r, respectively. These transcripts were terminated after 100-15...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.6.2479
更新日期：1987-03-25 00:00:00
abstract：:Pluripotency, the ability of embryonic stem cells to differentiate into specialized cell types, is determined by ESC-specific gene regulators such as transcription factors and chromatin modification factors. It is not well understood how ESCs are poised for differentiation, however, and methods are needed for prognosi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx752
更新日期：2017-10-13 00:00:00
abstract：:With recent advancements in techniques for cellular data acquisition, information on cellular processes has been increasing at a dramatic rate. Visualization is critical to analyzing and interpreting complex information; representing cellular processes or pathways is no exception. VISIBIOweb is a free, open-source, we...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq352
更新日期：2010-07-01 00:00:00
abstract：:Multicellular organismal development is controlled by a complex network of transcription factors, promoters and enhancers. Although reliable computational and experimental methods exist for enhancer detection, prediction of their target genes remains a major challenge. On the basis of available literature and ChIP-seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1081
更新日期：2011-04-01 00:00:00
abstract：:Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh736
更新日期：2004-08-03 00:00:00
abstract：:REDUCE is a motif-based regression method for microarray analysis. The only required inputs are (i) a single genome-wide set of absolute or relative mRNA abundances and (ii) the DNA sequence of the regulatory region associated with each gene that is probed. Currently supported organisms are yeast, worm and fly; it is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg630
更新日期：2003-07-01 00:00:00
abstract：:RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.23.7763
更新日期：1982-12-11 00:00:00
abstract：:Aminoglycosides antibiotics negate dissociation and recycling of the bacterial ribosome's subunits by binding to Helix 69 (H69) of 23S rRNA. The differential binding of various aminoglycosides to the chemically synthesized terminal domains of the Escherichia coli and human H69 has been characterized using spectroscopy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1253
更新日期：2010-05-01 00:00:00
abstract：:Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw528
更新日期：2016-10-14 00:00:00
abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.16.3121
更新日期：1996-08-15 00:00:00
abstract：:Successful development of biological databases requires accommodation of the burgeoning amounts of data from high-throughput genomics pipelines. As the volume of curated data in Animal QTLdb (https://www.animalgenome.org/QTLdb) increases exponentially, the resulting challenges must be met with rapid infrastructure dev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1084
更新日期：2019-01-08 00:00:00
abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw146
更新日期：2016-07-08 00:00:00
abstract：:The c-myc proto-oncogene encodes a highly unstable mRNA. Stabilized, truncated myc transcripts have been found in several human and murine tumors of hematopoietic origin. Recently, two tumors expressing 3' truncated c-myc mRNAs that were five times more stable than normal myc transcripts, were described. We have tried...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.9.2387
更新日期：1991-05-11 00:00:00
abstract：:chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression profiling. The server implements a probe selection algorithm that takes as an input, in addition to the target sequences...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq517
更新日期：2010-07-01 00:00:00
abstract：:The molecular mechanisms that underlie the neurological manifestations of patients with inherited diseases of vitamin B12 (cobalamin) metabolism remain to date obscure. We observed transcriptomic changes of genes involved in RNA metabolism and endoplasmic reticulum stress in a neuronal cell model with impaired cobalam...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky634
更新日期：2018-09-06 00:00:00
abstract：:ETS-domain transcription factors play important roles in controlling gene expression in a variety of different contexts; however, these proteins bind to very similar sites and it is unclear how in vivo specificity is achieved. In silico analysis is unlikely to reveal specific targets for individual family members and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp804
更新日期：2009-12-01 00:00:00
abstract：:We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Repl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.9.3083
更新日期：1977-09-01 00:00:00
abstract：:Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are lar...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.15.3851
更新日期：1981-08-11 00:00:00
abstract：:We present evidence that the splice sites in mammalian pre-mRNAs are brought together via a three dimensional diffusion mechanism. We tested two mechanisms for splice site pairing: a lateral diffusion ('scanning') model and the currently favored three dimensional diffusion ('jumping') model. Two lines of evidence that...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.9.1638
更新日期：1996-05-01 00:00:00
abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1613
更新日期：1998-04-01 00:00:00
abstract：:Protein-RNA interactions play essential roles in a number of regulatory mechanisms for gene expression such as RNA splicing, transport, translation and post-transcriptional control. As the number of available protein-RNA complex 3D structures has increased, it is now possible to statistically examine protein-RNA inter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl819
更新日期：2006-01-01 00:00:00
abstract：:The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of approximately 150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split at bot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl293
更新日期：2006-05-31 00:00:00
abstract：:We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.17.4055
更新日期：1993-08-25 00:00:00
abstract：:YdiV is a negative regulator of cell motility. It interacts with FlhD(4)C(2) complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV(2)-FlhD(2) complex at 1.9 Å and 2.9 Å resolutions, respectively....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks869
更新日期：2012-11-01 00:00:00
