Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports.

abstract：:HbVar (http://globin.bx.psu.edu/hbvar) is one of the oldest and most appreciated locus-specific databases launched in 2001 by a multi-center academic effort to provide timely information on the genomic alterations leading to hemoglobin variants and all types of thalassemia and hemoglobinopathies. Database records incl...

journal_title：Nucleic acids research

authors： Giardine B,Borg J,Viennas E,Pavlidis C,Moradkhani K,Joly P,Bartsakoulia M,Riemer C,Miller W,Tzimas G,Wajcman H,Hardison RC,Patrinos GP

更新日期：2014-01-01 00:00:00

abstract：:Resonances of the water exchangeable iminoprotons of the acceptor and D stem of yeast tRNAPhe have been assigned by means of Nuclear Overhauser Effects (NOE's). Assignments were made for spectra recorded from tRNA dialysed against a buffer with 110 mM sodium and 5 mM magnesium ions and against a buffer with 430 mM sod...

journal_title：Nucleic acids research

authors： Heerschap A,Haasnoot CA,Hilbers CW

更新日期：1982-11-11 00:00:00

abstract：:DNA binding of heat shock factor 2 (HSF2) is induced during hemin-induced differentiation of human erythroleukemia cell line K562. To identify the transcriptional activation and the regulatory domains of HSF2, we constructed a series of deletion derivatives fused to the yeast GAL4 DNA binding domain and analyzed their...

journal_title：Nucleic acids research

authors： Yoshima T,Yura T,Yanagi H

更新日期：1998-06-01 00:00:00

abstract：:The major surface antigen of procyclic and epimastigote forms of Trypanosoma congolense in the tsetse fly is GARP (glutamic acid/alanine-rich protein), which is thought to be the analogue of procyclin/PARP in Trypanosoma brucei. We have studied two T.congolense GARP loci (the 4.3 and 4.4 loci) whose transcription is a...

journal_title：Nucleic acids research

authors： Graham SV,Jefferies D,Barry JD

更新日期：1996-01-15 00:00:00

abstract：:We report the latest release (version 3.0) of the CATH protein domain database (http://www.cathdb.info). There has been a 20% increase in the number of structural domains classified in CATH, up to 86 151 domains. Release 3.0 comprises 1110 fold groups and 2147 homologous superfamilies. To cope with the increases in di...

journal_title：Nucleic acids research

authors： Greene LH,Lewis TE,Addou S,Cuff A,Dallman T,Dibley M,Redfern O,Pearl F,Nambudiry R,Reid A,Sillitoe I,Yeats C,Thornton JM,Orengo CA

更新日期：2007-01-01 00:00:00

abstract：:Augmented biological activity in vitro has been demonstrated in oligonucleotides (oligos) modified to provide nuclease resistance, to enhance cellular uptake or to increase target affinity. How chemical modification affects the duration of effect of an oligo with potent activity has not been investigated directly. We ...

journal_title：Nucleic acids research

authors： Tam RC,Li Y,Noonberg S,Hwang DG,Lui G,Hunt CA,Garovoy MR

更新日期：1994-03-25 00:00:00

abstract：:Various in vivo mutagenesis methods have been developed to facilitate fast and efficient continuous evolution of proteins in cells. However, they either modify the DNA region that does not match the target gene, or suffer from low mutation rates. Here, we report a mutator, eMutaT7 (enhanced MutaT7), with very fast in ...

journal_title：Nucleic acids research

authors： Park H,Kim S

更新日期：2021-01-06 00:00:00

abstract：:We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide...

journal_title：Nucleic acids research

authors： Chu BC,Orgel LE

更新日期：1988-05-11 00:00:00

abstract：:Differential display of mRNA is a simple, sensitive and powerful method to identify differentially expressed gene fragments. The main drawback of differential display is the lack of reproducibility and the inability to read and compare complex gels. This issue results from employing unoptimized primer combinations and...

journal_title：Nucleic acids research

authors： Malhotra K,Foltz L,Mahoney WC,Schueler PA

更新日期：1998-02-01 00:00:00

abstract：:The gene co-expression analysis toolbox (GeneCAT) introduces several novel microarray data analyzing tools. First, the multigene co-expression analysis, combined with co-expressed gene networks, provides a more powerful data mining technique than standard, single-gene co-expression analysis. Second, the high-throughpu...

journal_title：Nucleic acids research

authors： Mutwil M,Obro J,Willats WG,Persson S

更新日期：2008-07-01 00:00:00

abstract：:The IMGT/HLA database (http://www.ebi.ac.uk/imgt/hla) has provided a centralized repository for the sequences of the alleles named by the WHO Nomenclature Committee for Factors of the HLA System for the past four years. Since its initial release the database has grown and is the primary source of information for the s...

journal_title：Nucleic acids research

authors： Robinson J,Waller MJ,Parham P,de Groot N,Bontrop R,Kennedy LJ,Stoehr P,Marsh SG

更新日期：2003-01-01 00:00:00

abstract：:Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

journal_title：Nucleic acids research

authors： Lin Z,Hung KN,Grollman AP,de los Santos C

更新日期：1998-05-15 00:00:00

abstract：:We have used computer assisted dot matrix and oligonucleotide frequency analyses to identify highly recurring sequence elements of 7-11 base pairs in eukaryotic genes and viral DNAs. Such elements are found much more frequently than expected, often with an average spacing of a few hundred base pairs. Furthermore, the ...

journal_title：Nucleic acids research

authors： Bodnar JW,Ward DC

更新日期：1987-02-25 00:00:00

abstract：:The first step in gene expression, transcription, is modulated by the interaction of transcription factors with their corresponding binding sites on the DNA sequence. Pscan is a software tool that scans a set of sequences (e.g. promoters) from co-regulated or co-expressed genes with motifs describing the binding speci...

journal_title：Nucleic acids research

authors： Zambelli F,Pesole G,Pavesi G

更新日期：2009-07-01 00:00:00

abstract：:We have compared the properties of the poly(rA).oligo(dT) complex with those of the poly(rU).oligo(dA)n complex. Three main differences were found. First, poly(rA) and oligo(dT)n do not form a complex in concentrations of CsCl exceeding 2 M because the poly(rA) is insoluble in high salt. If the complex is made in low ...

journal_title：Nucleic acids research

authors： Mol JN,Borst P

更新日期：1976-04-01 00:00:00

abstract：:The prime mechanism by which p53 acts as a tumor suppressor is as a transcription factor regulating the expression of diverse downstream genes. The DNA-binding domain of p53 (p53DBD) interacts with defined DNA sites and is the main target for mutations in human primary tumors. Here, we show that the CWWG motif, found ...

journal_title：Nucleic acids research

authors： Beno I,Rosenthal K,Levitine M,Shaulov L,Haran TE

更新日期：2011-03-01 00:00:00

abstract：:The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiat...

journal_title：Nucleic acids research

authors： Okamoto T,Sugimoto K,Sugisaki H,Takanami M

更新日期：1977-07-01 00:00:00

abstract：:The structure of eukaryotic chromatin has been investigated by isolating and analyzing the "accessible" DNA fraction of rat liver chromatin. This DNA fraction has been isolated by titrating the chromatin with the protese-resistant D isomer of polylysine to bind the "accessible" DNA sites. After removal of chromosomal ...

journal_title：Nucleic acids research

authors： Arnold EA,Wahn U,Young KE

更新日期：1975-05-01 00:00:00

abstract：:The International Mouse Phenotyping Consortium (IMPC) web portal (http://www.mousephenotype.org) provides the biomedical community with a unified point of access to mutant mice and rich collection of related emerging and existing mouse phenotype data. IMPC mouse clinics worldwide follow rigorous highly structured and ...

journal_title：Nucleic acids research

authors： Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos

更新日期：2014-01-01 00:00:00

abstract：:Molecular beacons (MBs) have the potential to provide a powerful tool for rapid RNA detection in living cells, as well as monitoring the dynamics of RNA expression in response to external stimuli. To exploit this potential, it is necessary to distinguish true signal from background signal due to non-specific interacti...

journal_title：Nucleic acids research

authors： Rhee WJ,Bao G

更新日期：2010-05-01 00:00:00

abstract：:The Sleeping Beauty (SB) transposon is a promising gene transfer vector that integrates nonspecifically into host cell genomes. Herein, we attempt to direct transposon integration into predetermined DNA sites by coupling a site-specific DNA-binding domain (DBD) to the SB transposase. We engineered fusion proteins comp...

journal_title：Nucleic acids research

authors： Yant SR,Huang Y,Akache B,Kay MA

更新日期：2007-01-01 00:00:00

abstract：:DNA topoisomerases and DNA site-specific recombinases are biologically important enzymes involved in a diverse set of cellular processes. We show that replacement of a phosphodiester linkage by a 5'-bridging phosphorothioate linkage creates an efficient suicide substrate for calf thymus topoisomerase I and lambda inte...

journal_title：Nucleic acids research

authors： Burgin AB Jr,Huizenga BN,Nash HA

更新日期：1995-08-11 00:00:00

abstract：:A novel approach for the amplification of cDNA ends is described. It requires only minimal amounts of material, a simple cDNA synthesis reaction and a single PCR reaction to amplify the desired 5'- or 3'-ends of a certain cDNA of interest. It combines the so called CapFinder approach with solid phase cDNA synthesis, t...

journal_title：Nucleic acids research

authors： Schramm G,Bruchhaus I,Roeder T

更新日期：2000-11-15 00:00:00

abstract：:The double helix is known to form as a result of hybridization of complementary nucleic acid strands in aqueous solution. In the helix the negatively charged phosphate groups of each nucleic acid strand are distributed helically on the outside of the duplex and are available for interaction with cationic groups. Catio...

journal_title：Nucleic acids research

authors： Lemeshko SV,Powdrill T,Belosludtsev YY,Hogan M

更新日期：2001-07-15 00:00:00

abstract：:We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laborato...

journal_title：Nucleic acids research

authors： Goh CS,Lan N,Echols N,Douglas SM,Milburn D,Bertone P,Xiao R,Ma LC,Zheng D,Wunderlich Z,Acton T,Montelione GT,Gerstein M

更新日期：2003-06-01 00:00:00

abstract：:The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...

journal_title：Nucleic acids research

authors： D'Andrea D,Grassi L,Mazzapioda M,Tramontano A

更新日期：2013-07-01 00:00:00

abstract：:The cytosine base in DNA undergoes hydrolytic deamination at a considerable rate when UV radiation induces formation of a cyclobutane pyrimidine dimer (CPD) with an adjacent pyrimidine base. We have synthesized a phosphoramidite building block of a cis-syn cyclobutane thymine-uracil dimer (T[]U), which is the deaminat...

journal_title：Nucleic acids research

authors： Takasawa K,Masutani C,Hanaoka F,Iwai S

更新日期：2004-03-12 00:00:00

abstract：:E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 and pBR329. The proce...

journal_title：Nucleic acids research

authors： Dower WJ,Miller JF,Ragsdale CW

更新日期：1988-07-11 00:00:00

abstract：:Soil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spli...

journal_title：Nucleic acids research

authors： Gu J,Xia Z,Luo Y,Jiang X,Qian B,Xie H,Zhu JK,Xiong L,Zhu J,Wang ZY

更新日期：2018-02-28 00:00:00

abstract：:Mollusca represents the second largest animal phylum but remains poorly explored from a genomic perspective. While the recent increase in genomic resources holds great promise for a deep understanding of molluscan biology and evolution, access and utilization of these resources still pose a challenge. Here, we present...

journal_title：Nucleic acids research

authors： Liu F,Li Y,Yu H,Zhang L,Hu J,Bao Z,Wang S

更新日期：2021-01-08 00:00:00