Abstract:
:Transcription start and termination sites were mapped in the E. coli replication origin, oriC. Outward transcription from within oriC (promoters Pori-r and Pori-l) was found to start in vivo at position 178 for Pori-l and at positions 294 and 304 for Pori-r, respectively. These transcripts were terminated after 100-150 bases, at terminators designated Tori-l and Tori-r. Transcription from the 16 kd promoter, which lies clockwise adjacent to oriC and promotes transcription toward oriC, started at position 757 and gave transcripts with 3' ends at several positions within and to the left of the minimal replication origin. However, the majority of transcripts traversed the whole oriC region, and were not terminated within the DNA segment tested. Transcription of the chromosomal 16 kd gene was negatively regulated by DnaA protein and positively affected by dam methylation. The possible function of these transcripts is discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schauzu MA,Kücherer C,Kölling R,Messer W,Lother Hdoi
10.1093/nar/15.6.2479subject
Has Abstractpub_date
1987-03-25 00:00:00pages
2479-97issue
6eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::Triple helix-forming oligonucleotides covalently linked to topoisomerase I inhibitors, in particular the antitumor agent camptothecin, trigger topoisomerase I-mediated DNA cleavage selectively in the proximity of the binding site of the oligonucleotide vector. In the present study, we have performed a systematic analy...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2000-06-15 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2012-06-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2003-08-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00