The interaction of ribonuclease T 1 with DNA.

abstract：:Genomic DNA of calf thymus contains 1.5 times as much 5-methylcytosine as similar sperm DNA, but the major EcoRI repeat fragment from satellite I of thymus contains ten times as much 5-methylcytosine as the corresponding fragment from sperm DNA. Restriction enzyme analyses of the total DNA and the satellite I fragment...

journal_title：Nucleic acids research

authors： Sturm KS,Taylor JH

更新日期：1981-09-25 00:00:00

abstract：:The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...

journal_title：Nucleic acids research

authors： Szymanski M,Erdmann VA,Barciszewski J

更新日期：2007-01-01 00:00:00

abstract：:TFIIH is a multisubunit factor essential for transcription initiation and promoter escape of RNA polymerase II and for the opening of damaged DNA double strands in nucleotide excision repair (NER). In this study, we have analyzed at which step of the transcription cycle TFIIH is essential for transcription by RNA poly...

journal_title：Nucleic acids research

authors： Assfalg R,Lebedev A,Gonzalez OG,Schelling A,Koch S,Iben S

更新日期：2012-01-01 00:00:00

abstract：:A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Strongylocentrotus purpuratus has been characterized, and shown to contain the sequence features which indicate that ...

journal_title：Nucleic acids research

authors： Nisson PE,Hickey RJ,Boshar MF,Crain WR Jr

更新日期：1988-02-25 00:00:00

abstract：:Smad proteins transduce signals from transforming growth factor-beta (TGF-beta) receptors and regulate transcription of target genes. TGF-beta is implicated in the regulation of the smooth muscle cell specific gene SM22alpha, but little is known about how Smads are involved in SM22alpha gene transcription. In this rep...

journal_title：Nucleic acids research

authors： Chen S,Kulik M,Lechleider RJ

更新日期：2003-02-15 00:00:00

abstract：:A major goal of system biology is the characterization of transcription factors and microRNAs (miRNAs) and the transcriptional programs they regulate. We present Allegro, a method for de-novo discovery of cis-regulatory transcriptional programs through joint analysis of genome-wide expression data and promoter or 3' U...

journal_title：Nucleic acids research

authors： Halperin Y,Linhart C,Ulitsky I,Shamir R

更新日期：2009-04-01 00:00:00

abstract：:Structures of r(CGCGCG)2 and 2'-O-Me(CGCGCG)2 have been determined by NMR spectroscopy under low salt conditions. All protons and phosphorus nuclei resonances have been assigned. Signals of H5'/5" have been assigned stereospecifically. All 3JH,H and 3JP,H coupling constants have been measured. The structures were dete...

journal_title：Nucleic acids research

authors： Popenda M,Biala E,Milecki J,Adamiak RW

更新日期：1997-11-15 00:00:00

abstract：:Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a sel...

journal_title：Nucleic acids research

authors： Nagaoka M,Shiraishi Y,Sugiura Y

更新日期：2001-12-15 00:00:00

abstract：:In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA...

journal_title：Nucleic acids research

authors： Feriotto G,Mischiati C,Bianchi N,Rutigliano C,Giacomini P,Gambari R

更新日期：1995-05-25 00:00:00

abstract：:RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, fa...

journal_title：Nucleic acids research

authors： Balik A,Penn AC,Nemoda Z,Greger IH

更新日期：2013-01-01 00:00:00

abstract：:We have used computer assisted dot matrix and oligonucleotide frequency analyses to identify highly recurring sequence elements of 7-11 base pairs in eukaryotic genes and viral DNAs. Such elements are found much more frequently than expected, often with an average spacing of a few hundred base pairs. Furthermore, the ...

journal_title：Nucleic acids research

authors： Bodnar JW,Ward DC

更新日期：1987-02-25 00:00:00

abstract：:We have developed an effective post-PCR sterilization process and have applied the procedure to a diagnostic assay for HIV-1. The method, which is based on isopsoralen photochemistry, satisfies both the inactivation and hybridization requirements of a practical sterilization process. The key feature of the technique i...

journal_title：Nucleic acids research

authors： Isaacs ST,Tessman JW,Metchette KC,Hearst JE,Cimino GD

更新日期：1991-01-11 00:00:00

abstract：:The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce pADH06C. The yeast strains SX46A, 7799-4B and VV-6 are deficient in endogenous O6-alkylguanine-DNA-alkyltransferase and...

journal_title：Nucleic acids research

authors： Brozmanova J,Kleibl K,Vlckova V,Skorvaga M,Cernakova L,Margison GP

更新日期：1990-01-25 00:00:00

abstract：:PIWI-interacting RNAs (piRNAs) engage PIWI proteins to silence transposons and promote germ cell development in animals. In diverse species, piRNA biogenesis occurs near the mitochondrial surface, and involves mitochondrial membrane-anchored factors. In mice, two cytoplasmic PIWI proteins, MIWI and MILI, receive proce...

journal_title：Nucleic acids research

authors： Ding D,Liu J,Dong K,Melnick AF,Latham KE,Chen C

更新日期：2019-03-18 00:00:00

abstract：:The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These exp...

journal_title：Nucleic acids research

authors： Zhu QS,Heisterkamp N,Groffen J

更新日期：1990-12-11 00:00:00

abstract：:Single-cell RNA-sequencing (scRNA-seq) enables the characterization of transcriptomic profiles at the single-cell resolution with increasingly high throughput. However, it suffers from many sources of technical noises, including insufficient mRNA molecules that lead to excess false zero values, termed dropouts. Comput...

journal_title：Nucleic acids research

authors： Xu Y,Zhang Z,You L,Liu J,Fan Z,Zhou X

更新日期：2020-09-04 00:00:00

abstract：:Overlap-directed DNA assembly methods allow multiple DNA parts to be assembled together in one reaction. These methods, which rely on sequence homology between the ends of DNA parts, have become widely adopted in synthetic biology, despite being incompatible with a key principle of engineering: modularity. To answer t...

journal_title：Nucleic acids research

authors： Casini A,MacDonald JT,De Jonghe J,Christodoulou G,Freemont PS,Baldwin GS,Ellis T

更新日期：2014-01-01 00:00:00

abstract：:The promoter region of the mouse CCAAT-Enhancer Binding Protein (C/EBP alpha) gene is capable of directing high levels of expression of reporter constructs in various cell lines, albeit even in cells that do not express their endogenous C/EBP alpha gene. To understand the molecular mechanisms underlying this ubiquitou...

journal_title：Nucleic acids research

authors： Legraverend C,Antonson P,Flodby P,Xanthopoulos KG

更新日期：1993-04-25 00:00:00

abstract：:The detailed principles of the hierarchical folding of eukaryotic chromosomes have been revealed during the last two decades. Along with structures composing three-dimensional (3D) genome organization (chromatin compartments, topologically associating domains, chromatin loops, etc.), the molecular mechanisms that are ...

journal_title：Nucleic acids research

authors： Kantidze OL,Razin SV

更新日期：2020-05-21 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:Transcriptional regulation of the delta-aminolevulinic acid synthetase gene of Rhizobium meliloti was investigated under conditions of normal vegetative growth and during symbiosis with the legume host alfalfa. S1 nuclease mapping and DNA sequence analysis indicated that transcription originates from two sites separat...

journal_title：Nucleic acids research

authors： Leong SA,Williams PH,Ditta GS

更新日期：1985-08-26 00:00:00

abstract：:Arabidopsis microRNA (miRNA) genes (MIR) give rise to 20- to 22-nt miRNAs that are generated predominantly by the type III endoribonuclease Dicer-like 1 (DCL1) but do not require any RNA-dependent RNA Polymerases (RDRs) or RNA Polymerase IV (Pol IV). Here, we identify a novel class of non-conserved MIR genes that give...

journal_title：Nucleic acids research

authors： Chellappan P,Xia J,Zhou X,Gao S,Zhang X,Coutino G,Vazquez F,Zhang W,Jin H

更新日期：2010-11-01 00:00:00

abstract：:Mammalian mitochondrial ribosomes evolved from bacterial ribosomes by reduction of ribosomal RNAs, increase of ribosomal protein content, and loss of guanine nucleotides. Guanine is the base most sensitive to oxidative damage. By systematically comparing high-quality, small ribosomal subunit RNA sequence alignments an...

journal_title：Nucleic acids research

authors： Hosseini M,Roy P,Sissler M,Zirbel CL,Westhof E,Leontis N

更新日期：2018-11-16 00:00:00

abstract：:Bovine mitochondrial (mt) phenylalanine tRNA (tRNA(Phe)), which lacks the 'conserved' GG and T psi YCG sequences, was efficiently purified by the selective hybridization method using a solid phase DNA probe. The entire nucleotide sequence of the tRNA, including modified nucleotides, was determined and its higher-order...

journal_title：Nucleic acids research

authors： Wakita K,Watanabe Y,Yokogawa T,Kumazawa Y,Nakamura S,Ueda T,Watanabe K,Nishikawa K

更新日期：1994-02-11 00:00:00

abstract：:Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

journal_title：Nucleic acids research

authors： Hackney JA,Ehrenkaufer GM,Singh U

更新日期：2007-01-01 00:00:00

abstract：:We attempted to use the polymerase chain reaction (PCR) to monitor in vitro recombination in a plasmid containing directly repeated sequences. Some of the plasmid preparations which had not been exposed to recombination conditions were however found to behave in the PCR test as if they had undergone homologous recombi...

journal_title：Nucleic acids research

authors： Marton A,Delbecchi L,Bourgaux P

更新日期：1991-05-11 00:00:00

abstract：:Conserved in a variety of evolutionarily divergent plant species, LOB DOMAIN (LBD) genes define a large, plant-specific family of largely unknown function. LBD genes have been implicated in a variety of developmental processes in plants, although to date, relatively few members have been assigned functions. LBD protei...

journal_title：Nucleic acids research

authors： Husbands A,Bell EM,Shuai B,Smith HM,Springer PS

更新日期：2007-01-01 00:00:00

abstract：:Human Adenovirus type 5 encodes two short RNA polymerase III transcripts, the virus-associated (VA) RNAI and VA RNAII, which can adopt stable hairpin structures that resemble micro-RNA precursors. The terminal stems of the VA RNAs are processed into small RNAs (mivaRNAs) that are incorporated into RISC. It has been re...

journal_title：Nucleic acids research

authors： Xu N,Gkountela S,Saeed K,Akusjärvi G

更新日期：2009-11-01 00:00:00

abstract：:Properties of a mutant bacteriophage T2 DNA [N:(6)-adenine] methyltransferase (T2 Dam MTase) have been investigated for its potential utilization in RecA-assisted restriction endonuclease (RARE) cleavage. Steady-state kinetic analyses with oligonucleotide duplexes revealed that, compared to wild-type T4 Dam, both wild...

journal_title：Nucleic acids research

authors： Minko I,Hattman S,Lloyd RS,Kossykh V

更新日期：2001-04-01 00:00:00

abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...

journal_title：Nucleic acids research

authors： Burel SA,Hart CE,Cauntay P,Hsiao J,Machemer T,Katz M,Watt A,Bui HH,Younis H,Sabripour M,Freier SM,Hung G,Dan A,Prakash TP,Seth PP,Swayze EE,Bennett CF,Crooke ST,Henry SP

更新日期：2016-03-18 00:00:00