Abstract:
:RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, facilitating fine tuning and homeostasis of cellular functions, a similar regulation has not been described for RNA editing. Here, we show that the AMPA receptor R/G editing site is dynamically regulated in the hippocampus in response to activity. These changes are bi-directional, reversible and correlate with levels of the editase Adar2. This regulation is observed in the CA1 hippocampal subfield but not in CA3 and is thus subfield/celltype-specific. Moreover, alternative splicing of the flip/flop cassette downstream of the R/G site is closely linked to the editing state, which is regulated by Ca(2+). Our data show that A-to-I RNA editing has the capacity to tune protein function in response to external stimuli.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Balik A,Penn AC,Nemoda Z,Greger IHdoi
10.1093/nar/gks1045subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1124-34issue
2eissn
0305-1048issn
1362-4962pii
gks1045journal_volume
41pub_type
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