Carboxy terminal modifications of the P0 protein reveal alternative mechanisms of nuclear ribosomal stalk assembly.

abstract：:We have recently described three group I introns inserted into a single gene, orf142, of the staphylococcal bacteriophage Twort and suggested the presence of at least two additional self-splicing introns in this phage genome. Here we report that two previously uncharacterized introns, 429 and 1087 nt in length, interr...

journal_title：Nucleic acids research

authors： Landthaler M,Begley U,Lau NC,Shub DA

更新日期：2002-05-01 00:00:00

abstract：:Circular DNA aptamers are powerful candidates for therapeutic applications given their dramatically enhanced biostability. Herein we report the first effort to evolve circular DNA aptamers that bind a human protein directly in serum, a complex biofluid. Targeting human thrombin, this strategy has led to the discovery ...

journal_title：Nucleic acids research

authors： Mao Y,Gu J,Chang D,Wang L,Yao L,Ma Q,Luo Z,Qu H,Li Y,Zheng L

更新日期：2020-11-04 00:00:00

abstract：:Cell-free extracts employed as chromatin assembly systems contain a myriad of proteins, polyanions and nucleic acids. The roles of ATP, MgCl2 and other cofactors in the catalysis of nucleosome formation by the Xenopus laevis oocyte S-150 have yet to be established unequivocally. In this study we examine the influence ...

journal_title：Nucleic acids research

authors： Sekiguchi JM,Kmiec EB

更新日期：1992-02-25 00:00:00

abstract：:Natural oligopeptides may regulate nearly all vital processes. To date, the chemical structures of nearly 6000 oligopeptides have been identified from >1000 organisms representing all the biological kingdoms. We have compiled the known physical, chemical and biological properties of these oligopeptides--whether synthe...

journal_title：Nucleic acids research

authors： Zamyatnin AA,Borchikov AS,Vladimirov MG,Voronina OL

更新日期：2006-01-01 00:00:00

abstract：:One challenge in engineering organisms is taking responsibility for their behavior over many generations. Spontaneous mutations arising before or during use can impact heterologous genetic functions, disrupt system integration, or change organism phenotype. Here, we propose restructuring the genetic code itself such t...

journal_title：Nucleic acids research

authors： Calles J,Justice I,Brinkley D,Garcia A,Endy D

更新日期：2019-11-04 00:00:00

abstract：:U4 RNA from chicken, rat and man was examined for nucleotide sequence and secondary structure. Three molecular species, U4A, U4B and U4C were detected in the three animal species. U4A is 146 nucleotide long and U4B RNA only lacks the 3' terminal G. four nucleotides are missing at the 3'-end of U4C RNA which, in additi...

journal_title：Nucleic acids research

authors： Krol A,Branlant C,Lazar E,Gallinaro H,Jacob M

更新日期：1981-06-25 00:00:00

abstract：:Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination fa...

journal_title：Nucleic acids research

authors： Yue L,Li J,Zhang B,Qi L,Li Z,Zhao F,Li L,Zheng X,Dong X

更新日期：2020-09-25 00:00:00

abstract：:A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in the following order: (5') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3') (the subscripts giv...

journal_title：Nucleic acids research

authors： Bavykin SG,Usachenko SI,Lishanskaya AI,Shick VV,Belyavsky AV,Undritsov IM,Strokov AA,Zalenskaya IA,Mirzabekov AD

更新日期：1985-05-24 00:00:00

abstract：:A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removin...

journal_title：Nucleic acids research

authors： Kalmár T,Bachrati CZ,Marcsik A,Raskó I

更新日期：2000-06-15 00:00:00

abstract：:The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric DNA. Herein, we developed an EM-PCR and TA cloning-based approach to s...

journal_title：Nucleic acids research

authors： Zhang X,Zhang Y,Zhang W

更新日期：2020-07-09 00:00:00

abstract：:Topoisomerase II is an essential enzyme that is required for virtually every process that requires movement of DNA within the nucleus or the opening of the double helix. This enzyme helps to regulate DNA under- and overwinding and removes knots and tangles from the genetic material. In order to carry out its critical ...

journal_title：Nucleic acids research

authors： Deweese JE,Osheroff N

更新日期：2009-02-01 00:00:00

abstract：:The higher order structure of the functionally important 530 loop in Escherichia coli 16S rRNA was studied in mutants with single base changes at position 517, which significantly impair translational fidelity. The 530 loop has been proposed to interact with the EF-Tu-GTP-aatRNA ternary complex during decoding. The re...

journal_title：Nucleic acids research

authors： Van Ryk DI,Dahlberg AE

更新日期：1995-09-11 00:00:00

abstract：:The discovery of hydroxymethyl-, formyl- and carboxylcytosine, generated through oxidation of methylcytosine by TET dioxygenases, raised the question how these modifications contribute to epigenetic regulation. As they are subjected to complex regulation in vivo, we dissected links to gene expression with in vitro mod...

journal_title：Nucleic acids research

authors： Müller U,Bauer C,Siegl M,Rottach A,Leonhardt H

更新日期：2014-07-01 00:00:00

abstract：:Mutations in mammalian and Drosophila Hel308 and PolQ paralogues cause genome instability but their helicase functions are mysterious. By in vivo and in vitro analysis, we show that Hel308 from archaea (Hel308a) may act at stalled replication forks. Introducing hel308a into Escherichia coli dnaE strains that condition...

journal_title：Nucleic acids research

authors： Guy CP,Bolt EL

更新日期：2005-06-30 00:00:00

abstract：:In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme,...

journal_title：Nucleic acids research

authors： Tay Y,Ho C,Droge P,Ghadessy FJ

更新日期：2010-03-01 00:00:00

abstract：:The Genomes Online Database (GOLD) (https://gold.jgi.doe.gov) is an open online resource, which maintains an up-to-date catalog of genome and metagenome projects in the context of a comprehensive list of associated metadata. Information in GOLD is organized into four levels: Study, Biosample/Organism, Sequencing Proje...

journal_title：Nucleic acids research

authors： Mukherjee S,Stamatis D,Bertsch J,Ovchinnikova G,Katta HY,Mojica A,Chen IA,Kyrpides NC,Reddy T

更新日期：2019-01-08 00:00:00

abstract：:A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences ...

journal_title：Nucleic acids research

authors： Xia YN,Burbank DE,Uher L,Rabussay D,Van Etten JL

更新日期：1987-08-11 00:00:00

abstract：:RNA polymerase III recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo(rU) synthesis within this sequence, and releases the RNA. The pol III subunit Rpc11p (C11) mediates RNA 3'-5' cleavage in the catalytic center of pol III during pausing. The amino and carboxyl region...

journal_title：Nucleic acids research

authors： Iben JR,Mazeika JK,Hasson S,Rijal K,Arimbasseri AG,Russo AN,Maraia RJ

更新日期：2011-08-01 00:00:00

abstract：:Nuclear extracts prepared from growth hormone-secreting (GC) and prolactin-secreting (235-1) rat anterior pituitary cell lines were compared for their ability to bind to the DNA sequences conferring tissue-specificity to the expression of the rat growth hormone (rGH) gene promoter. Cell-specific differences in the int...

journal_title：Nucleic acids research

authors： Schaufele F,West BL,Reudelhuber T

更新日期：1990-09-11 00:00:00

abstract：:Single-stranded mRNA molecules form secondary structures through complementary self-interactions. Several hypotheses have been proposed on the relationship between the nucleotide sequence, encoded amino acid sequence and mRNA secondary structure. We performed the first transcriptome-wide in silico analysis of the huma...

journal_title：Nucleic acids research

authors： Shabalina SA,Ogurtsov AY,Spiridonov NA

更新日期：2006-05-08 00:00:00

abstract：:Slime mold, plant and insect dihydropyrimidine amidohydrolases (DHPases, EC 3.5.2.2), which catalyze the second step of pyrimidine and several anti-cancer drug degradations, were cloned and shown to functionally replace a defective DHPase enzyme in the yeast Saccharomyces kluyveri. The yeast and slime mold DHPases wer...

journal_title：Nucleic acids research

authors： Gojkovic Z,Rislund L,Andersen B,Sandrini MP,Cook PF,Schnackerz KD,Piskur J

更新日期：2003-03-15 00:00:00

abstract：:The mRNAs for different variant surface antigens of Trypanosoma brucei start with the same 35 nucleotides. This sequence is encoded by a separate mini-exon, located in a 1.35-kb repetitive element. We have reported that trypanosomes contain many transcripts that hybridize to mini-exon probes, even if they do not make ...

journal_title：Nucleic acids research

authors： De Lange T,Michels PA,Veerman HJ,Cornelissen AW,Borst P

更新日期：1984-05-11 00:00:00

abstract：:Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host and make available such data to the community, but these databases are frequently limited in scope and/or content. BioMuta, a database of cancer-associated si...

journal_title：Nucleic acids research

authors： Dingerdissen HM,Torcivia-Rodriguez J,Hu Y,Chang TC,Mazumder R,Kahsay R

更新日期：2018-01-04 00:00:00

abstract：:Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely rela...

journal_title：Nucleic acids research

authors： Khusainov I,Vicens Q,Bochler A,Grosse F,Myasnikov A,Ménétret JF,Chicher J,Marzi S,Romby P,Yusupova G,Yusupov M,Hashem Y

更新日期：2016-12-01 00:00:00

abstract：:Lambda-red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) gene, which enc...

journal_title：Nucleic acids research

authors： Wong QN,Ng VC,Lin MC,Kung HF,Chan D,Huang JD

更新日期：2005-03-30 00:00:00

abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...

journal_title：Nucleic acids research

authors： Seurinck J,Van de Voorde A,Van Montagu M

更新日期：1983-07-11 00:00:00

abstract：:Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression tha...

journal_title：Nucleic acids research

authors： Kubo M,Nishiyama T,Tamada Y,Sano R,Ishikawa M,Murata T,Imai A,Lang D,Demura T,Reski R,Hasebe M

更新日期：2019-05-21 00:00:00

abstract：:Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...

journal_title：Nucleic acids research

authors： Grin I,Ishchenko AA

更新日期：2016-05-05 00:00:00

abstract：:Two new beta-lactoglobulin (BLG)/human serum albumin (HSA) hybrid gene vectors were constructed and tested for expression in COS-7 cells and in transgenic mice. The HSA sequences were inserted between the second and sixth BLG exons. Transient transfection experiments with these vectors as well as a series of additiona...

journal_title：Nucleic acids research

authors： Barash I,Nathan M,Kari R,Ilan N,Shani M,Hurwitz DR

更新日期：1996-02-15 00:00:00

abstract：:A 5',7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and a textbook aspect of co-transcriptional RNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Interest in the CBC has recently renewed...

journal_title：Nucleic acids research

authors： Dou Y,Kalmykova S,Pashkova M,Oghbaie M,Jiang H,Molloy KR,Chait BT,Rout MP,Fenyö D,Jensen TH,Altukhov I,LaCava J

更新日期：2020-10-09 00:00:00