Abstract:
:Bacillus subtilis RecN appears to be an early detector of breaks in double-stranded DNA. In vivo, RecN forms discrete nucleoid-associated structures and in vitro exhibits Mg2+-dependent single-stranded (ss) DNA binding and ssDNA-dependent ATPase activities. In the presence of ATP or ADP, RecN assembles to form large networks with ssDNA molecules (designated complexes CII and CIII) that involve ATP binding and requires a 3'-OH at the end of ssDNA molecule. Addition of dATP-RecA complexes dissociates RecN from these networks, but this is not observed following addition of an ssDNA binding protein. Apparently, ATP modulates the RecN-ssDNA complex for binding to ssDNA extensions and, in vivo, RecN-ATP bound to 3'-ssDNA might sequester ssDNA ends within complexes that protect the ssDNA while the RecA accessory proteins recruit RecA. With the association of RecA to ssDNA, RecN would dissociate from the DNA end facilitating the subsequent steps in DNA repair.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sanchez H,Alonso JCdoi
10.1093/nar/gki533keywords:
subject
Has Abstractpub_date
2005-04-22 00:00:00pages
2343-50issue
7eissn
0305-1048issn
1362-4962pii
33/7/2343journal_volume
33pub_type
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