CYCLONET--an integrated database on cell cycle regulation and carcinogenesis.

abstract：:2'-O-(2-methoxyethyl) (2'-MOE) RNA possesses favorable pharmocokinetic properties that make it a promising option for the design of oligonucleotide drugs. Telomerase is a ribonucleoprotein that is up-regulated in many types of cancer, but its potential as a target for chemotherapy awaits the development of potent and ...

journal_title：Nucleic acids research

authors： Elayadi AN,Demieville A,Wancewicz EV,Monia BP,Corey DR

更新日期：2001-04-15 00:00:00

abstract：:A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern....

journal_title：Nucleic acids research

authors： Argos P

更新日期：1988-11-11 00:00:00

abstract：:Generation of double-stranded cDNA during reverse transcription of a variety of mRNA molecules is well known to involve the formation of covalently linked antisense and sense strands in a hairpin configuration. In the present study we have examined the sequence of molecular events which occurs during cDNA synthesis fr...

journal_title：Nucleic acids research

authors： Volloch VZ,Schweitzer B,Rits S

更新日期：1994-12-11 00:00:00

abstract：:The Exon/Intron Database (ExInt) stores information of all GenBank eukaryotic entries containing an annotated intron sequence. Data are available through a retrieval system, as flat-files and as a MySQL dump file. In this report we discuss several implementations added to ExInt, which is accessible at http://intron.bi...

journal_title：Nucleic acids research

authors： Sakharkar M,Passetti F,de Souza JE,Long M,de Souza SJ

更新日期：2002-01-01 00:00:00

abstract：:Bovine mitochondrial (mt) phenylalanine tRNA (tRNA(Phe)), which lacks the 'conserved' GG and T psi YCG sequences, was efficiently purified by the selective hybridization method using a solid phase DNA probe. The entire nucleotide sequence of the tRNA, including modified nucleotides, was determined and its higher-order...

journal_title：Nucleic acids research

authors： Wakita K,Watanabe Y,Yokogawa T,Kumazawa Y,Nakamura S,Ueda T,Watanabe K,Nishikawa K

更新日期：1994-02-11 00:00:00

abstract：:Using peptide arrays and binding to native histone proteins, we show that the ADD domain of Dnmt3a specifically interacts with the H3 histone 1-19 tail. Binding is disrupted by di- and trimethylation of K4, phosphorylation of T3, S10 or T11 and acetylation of K4. We did not observe binding to the H4 1-19 tail. The ADD...

journal_title：Nucleic acids research

authors： Zhang Y,Jurkowska R,Soeroes S,Rajavelu A,Dhayalan A,Bock I,Rathert P,Brandt O,Reinhardt R,Fischle W,Jeltsch A

更新日期：2010-07-01 00:00:00

abstract：:Telomeric DNAs consist of tandem repeats of G-clusters such as TTAGGG and TG1-3, which are the human and yeast repeat sequences, respectively. In the yeast Saccharomyces cerevisiae, the telomeric repeats are non-nucleosomal, whereas in humans, they are organized in tightly packaged nucleosomes. However, previous in vi...

journal_title：Nucleic acids research

authors： Ichikawa Y,Morohashi N,Nishimura Y,Kurumizaka H,Shimizu M

更新日期：2014-02-01 00:00:00

abstract：:Liquid-liquid phase separation plays an important role in a variety of cellular processes, including the formation of membrane-less organelles, the cytoskeleton, signalling complexes, and many other biological supramolecular assemblies. Studies on the molecular basis of phase separation in cells have focused on protei...

journal_title：Nucleic acids research

authors： Zhang Y,Yang M,Duncan S,Yang X,Abdelhamid MAS,Huang L,Zhang H,Benfey PN,Waller ZAE,Ding Y

更新日期：2019-12-16 00:00:00

abstract：:The genomic distribution of the abundant eukaryotic d(GA x TC)(n) DNA microsatellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombination in SV40 minichromosomes, the rate of homologous DNA recombination increasing by as much...

journal_title：Nucleic acids research

authors： Benet A,Mollà G,Azorín F

更新日期：2000-12-01 00:00:00

abstract：:Genome packing in adenovirus has long evaded precise description, since the viral dsDNA molecule condensed by proteins (core) lacks icosahedral order characteristic of the virus protein coating (capsid). We show that useful insights regarding the organization of the core can be inferred from the analysis of spatial di...

journal_title：Nucleic acids research

authors： Pérez-Berná AJ,Marion S,Chichón FJ,Fernández JJ,Winkler DC,Carrascosa JL,Steven AC,Šiber A,San Martín C

更新日期：2015-04-30 00:00:00

abstract：:Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (sha...

journal_title：Nucleic acids research

authors： Chiu TP,Rao S,Mann RS,Honig B,Rohs R

更新日期：2017-12-01 00:00:00

abstract：:The plant gene enod40 is highly conserved among legumes and also present in various non-legume species. It is presumed to play a central regulatory role in the Rhizobium-legume interaction, being expressed well before the initiation of cortical cell divisions resulting in nodule formation. Two small peptides encoded b...

journal_title：Nucleic acids research

authors： Girard G,Roussis A,Gultyaev AP,Pleij CW,Spaink HP

更新日期：2003-09-01 00:00:00

abstract：:Polyketide synthases (PKSs) catalyze biosynthesis of a diverse family of pharmaceutically important secondary metabolites. Bioinformatics analysis of sequence and structural features of PKS proteins plays a crucial role in discovery of new natural products by genome mining, as well as in design of novel secondary meta...

journal_title：Nucleic acids research

authors： Anand S,Prasad MV,Yadav G,Kumar N,Shehara J,Ansari MZ,Mohanty D

更新日期：2010-07-01 00:00:00

abstract：:We have examined the transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 in UV irradiated cells, in the mitotic cell cycle, and during meiosis. Levels of RAD18 mRNA increased upon UV irradiation, but remained constant during the mitotic cell cycle. During meiosis, RAD18 mRNA levels rose about 4 fol...

journal_title：Nucleic acids research

authors： Jones JS,Prakash L

更新日期：1991-02-25 00:00:00

abstract：:Synthesis of HIV GagPol involves a proportion of ribosomes translating a U6A shift site at the distal end of the gag gene performing a programmed -1 ribosomal frameshift event to enter the overlapping pol gene. In vitro studies here show that at the same shift motif HIV reverse transcriptase generates -1 and +1 indels...

journal_title：Nucleic acids research

authors： Penno C,Kumari R,Baranov PV,van Sinderen D,Atkins JF

更新日期：2017-09-29 00:00:00

abstract：:We report for the first time an analysis of the ATPase activity of human DNA topoisomerase (topo) IIbeta. We show that topo IIbeta is a DNA-dependent ATPase that appears to fit Michaelis-Menten kinetics. The ATPase activity is stimulated 44-fold by DNA. The k(cat) for ATP hydrolysis by human DNA topo IIbeta in the pre...

journal_title：Nucleic acids research

authors： West KL,Turnbull RM,Willmore E,Lakey JH,Austin CA

更新日期：2002-12-15 00:00:00

abstract：:The Ensembl project has been aggregating, processing, integrating and redistributing genomic datasets since the initial releases of the draft human genome, with the aim of accelerating genomics research through rapid open distribution of public data. Large amounts of raw data are thus transformed into knowledge, which...

journal_title：Nucleic acids research

authors： Zerbino DR,Achuthan P,Akanni W,Amode MR,Barrell D,Bhai J,Billis K,Cummins C,Gall A,Girón CG,Gil L,Gordon L,Haggerty L,Haskell E,Hourlier T,Izuogu OG,Janacek SH,Juettemann T,To JK,Laird MR,Lavidas I,Liu Z,Lovel

更新日期：2018-01-04 00:00:00

abstract：:TFIIB plays a pivotal role during assembly of the RNA polymerase II transcription preinitiation complex. TFIIB is composed of two domains that engage in an intramolecular interaction that can be disrupted by the VP16 activation domain. In this study, we describe a novel human TFIIB derivative harbouring two point muta...

journal_title：Nucleic acids research

authors： Glossop JA,Dafforn TR,Roberts SG

更新日期：2004-03-22 00:00:00

abstract：:Modified bases, such as O6-methylguanines, are produced in cells exposed to alkylating agents and cause apoptosis. In human cells treated with N-methyl-N-nitrosourea, we detected a protein complex composed of MutSalpha, MutLalpha and PCNA on damaged DNA by immunoprecipitation method using chromatin extracts, in which ...

journal_title：Nucleic acids research

authors： Hidaka M,Takagi Y,Takano TY,Sekiguchi M

更新日期：2005-10-04 00:00:00

abstract：:Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein...

journal_title：Nucleic acids research

authors： Bryant KF,Cox JC,Wang H,Hogle JM,Ellington AD,Coen DM

更新日期：2005-10-24 00:00:00

abstract：:The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity...

journal_title：Nucleic acids research

authors： Felber BK,Gerber-Huber S,Meier C,May FE,Westley B,Weber R,Ryffel GU

更新日期：1981-06-11 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves of Digitaria setigera. This study has confirmed that both spliced and unspliced RNAs are synthesised by the same transcription unit. The position of the intron...

journal_title：Nucleic acids research

authors： Mullineaux PM,Guerineau F,Accotto GP

更新日期：1990-12-25 00:00:00

abstract：:Each of the major U snRNP polypeptides from human cells was purified by electroelution from SDS-polyacrylamide gels. Rabbit antisera could be obtained against the individual proteins 70K, A, B', B and D, although rabbits failed to elicit antibodies against E, F and G. A strong structural homology was found between pro...

journal_title：Nucleic acids research

authors： Reuter R,Rothe S,Lührmann R

更新日期：1987-05-26 00:00:00

abstract：:Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant ba...

journal_title：Nucleic acids research

authors： Li N,Chen Y,Guo Q,Zhang Y,Yuan Y,Ma C,Deng H,Lei J,Gao N

更新日期：2013-08-01 00:00:00

abstract：:The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...

journal_title：Nucleic acids research

authors： Böhlke K,Pisani FM,Vorgias CE,Frey B,Sobek H,Rossi M,Antranikian G

更新日期：2000-10-15 00:00:00

abstract：:The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiatio...

journal_title：Nucleic acids research

authors： Pickett GG,Peabody DS

更新日期：1993-09-25 00:00:00

abstract：:The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domai...

journal_title：Nucleic acids research

authors： Weston K

更新日期：1992-06-25 00:00:00

abstract：:DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-ba...

journal_title：Nucleic acids research

authors： Ju J,Glazer AN,Mathies RA

更新日期：1996-03-15 00:00:00

abstract：:The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR...

journal_title：Nucleic acids research

authors： Bruder JT,Heidecker G,Tan TH,Weske JC,Derse D,Rapp UR

更新日期：1993-11-11 00:00:00

abstract：:Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chro...

journal_title：Nucleic acids research

authors： Kubli E,Schmidt T

更新日期：1978-05-01 00:00:00