Abstract：
:A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern. The aspartates may be important in polymerase function by acting directly in catalysis and/or by binding magnesium.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Argos Pdoi
10.1093/nar/16.21.9909subject
Has Abstractpub_date
1988-11-11 00:00:00pages
9909-16issue
21eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract：:The chromatin structure of a germ-line specific gene, the TPG-3 gene, one of the rainbow trout protamine genes was analyzed in various tissues. The protamine genes are expressed in early stage testis but not in late stage testis, liver or erythrocyte. Five potential CpG methylation sites in the coding and flanking reg...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.8.3385
更新日期：1987-04-24 00:00:00
abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.14.2815
更新日期：2000-07-15 00:00:00
abstract：:We have carried out an extended set of standard and enhanced-sampling MD simulations (for a cumulative simulation time of 620 μs) with the aim to study folding landscapes of the rGGGUUAGGG and rGGGAGGG parallel G-hairpins (PH) with propeller loop. We identify folding and unfolding pathways of the PH, which is bridged ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky712
更新日期：2018-09-28 00:00:00
abstract：:TGGCA-binding proteins are nuclear proteins with high affinity for double-stranded DNA homologous to the prototype recognition sequence 5'YTGGCANNNTGCCAR 3'. Their ubiquitous tissue distribution in higher vertebrates characterizes them as a class of highly conserved proteins which may exert a basic function. To obtain...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.6.2045
更新日期：1985-03-25 00:00:00
abstract：:Isopentenyl adenosine derivatives are always located adjacent to the 3' end of the anticodon in transfer RNA and have been implicated in certain biological functions. In the enteric bacterium, E. coli, the derivative is ms2i6A whereas in some plant associated bacteria the derivative is the hydroxylated form, ms2io6A. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.18.5663
更新日期：1982-09-25 00:00:00
abstract：:We describe mGene.web, a web service for the genome-wide prediction of protein coding genes from eukaryotic DNA sequences. It offers pre-trained models for the recognition of gene structures including untranslated regions in an increasing number of organisms. With mGene.web, users have the additional possibility to tr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp479
更新日期：2009-07-01 00:00:00
abstract：:A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks451
更新日期：2012-08-01 00:00:00
abstract：:The rat alpha- and bovine alpha s1-casein genes have been isolated and their 5' sequences determined. The rat alpha-, beta-, gamma- and bovine alpha s1-casein genes contain similar 5' exon arrangements in which the 5' noncoding, signal peptide and casein kinase phosphorylation sequences are each encoded by separate ex...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.4.1883
更新日期：1986-02-25 00:00:00
abstract：:To address how eukaryotic replication forks respond to fork stalling caused by strong non-covalent protein-DNA barriers, we engineered the controllable Fob-block system in Saccharomyces cerevisiae. This system allows us to strongly induce and control replication fork barriers (RFB) at their natural location within the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt051
更新日期：2013-03-01 00:00:00
abstract：:Chemically modified antisense oligonucleotides (ASOs) with phosphorothioate (PS) linkages have been extensively studied as research and therapeutic agents. PS-ASOs can enter the cell and trigger cleavage of complementary RNA by RNase H1 even in the absence of transfection reagent. A number of cell surface proteins hav...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky145
更新日期：2018-04-20 00:00:00
abstract：:The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.15.2982
更新日期：1994-08-11 00:00:00
abstract：:The Bovine Genome Database (BGD) (http://bovinegenome.org) has been the key community bovine genomics database for more than a decade. To accommodate the increasing amount and complexity of bovine genomics data, BGD continues to advance its practices in data acquisition, curation, integration and efficient data retrie...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz944
更新日期：2020-01-08 00:00:00
abstract：:The synthesis of stable RNA in Mycoplasma capricolum was studied by [32P] labeling of cellular RNA of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. The results indicate that M. capricolum employs the same stringent control mechanism used by E. coli cells, as ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.15.3641
更新日期：1981-08-11 00:00:00
abstract：:G-quadruplexes (G4s) are stable secondary structures that can lead to the stalling of replication forks and cause genomic instability. Pif1 is a 5' to 3' helicase, localized to both the mitochondria and nucleus that can unwind G4s in vitro and prevent fork stalling at G4 forming sequences in vivo. Using in vitro prime...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz608
更新日期：2019-09-19 00:00:00
abstract：:Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa163
更新日期：2020-05-07 00:00:00
abstract：:The development of complex methods in molecular biology is a laborious, costly, iterative and often intuition-bound process where optima are sought in a multidimensional parameter space through step-by-step optimizations. The difficulty of miniaturizing reactions under the microliter volumes usually handled in multiwe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa079
更新日期：2020-04-17 00:00:00
abstract：:The role of Ribonuclease H1 (RNase H1) during primer removal and ligation at the mitochondrial origin of light-strand DNA synthesis (OriL) is a key, yet poorly understood, step in mitochondrial DNA maintenance. Here, we reconstitute the replication cycle of L-strand synthesis in vitro using recombinant mitochondrial p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky708
更新日期：2018-10-12 00:00:00
abstract：:It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.11.3679
更新日期：1983-06-11 00:00:00
abstract：:Predictably regulating protein expression levels to improve recombinant protein production has become an important tool, but is still rarely applied to engineer mammalian cells. We therefore sought to set-up an easy-to-implement toolbox to facilitate fast and reliable regulation of protein expression in mammalian cell...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa847
更新日期：2020-11-18 00:00:00
abstract：:Most small nucleolar RNAs (snoRNAs) guide rRNA nucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions. These activities involve direct base-pairing of the snoRNA with pre-rRNA using different domains. It is not known if the modification and processing functions occur independen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq043
更新日期：2010-06-01 00:00:00
abstract：:A novel method of determining the number of superhelical turns of a covalently-closed plasmid DNA is described. It relies on the determination of the hyperchromicity, and hence the proportion of unstacked basepairs, of a partially heat-denatured sample which co-migrates during electrophoresis with nicked circular dupl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.2.525
更新日期：1982-01-22 00:00:00
abstract：:NMR data from different experiments often contain errors; thus, automated backbone resonance assignment is a very challenging issue. In this paper, we present a method called GANA that uses a genetic algorithm to automatically perform backbone resonance assignment with a high degree of precision and recall. Precision ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki768
更新日期：2005-08-10 00:00:00
abstract：:DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To streamline automation...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx132
更新日期：2017-06-20 00:00:00
abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn859
更新日期：2009-01-01 00:00:00
abstract：:Bcg I and Bcg I-like restriction endonucleases cleave double stranded DNA specifically on both sides of their asymmetric recognition sequences which are interrupted by several ambiguous base pairs. Their heterosubunit structure, bifunctionality and stimulation by AdoMet make them different from other classified restri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.22.4444
更新日期：1997-11-15 00:00:00
abstract：:It has been postulated that a myriad of long noncoding RNAs (lncRNAs) contribute to gene regulation. In fission yeast, glucose starvation triggers lncRNA transcription across promoter regions of stress-responsive genes including fbp1 (fructose-1,6-bisphosphatase1). At the fbp1 promoter, this transcription promotes chr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw142
更新日期：2016-06-20 00:00:00
abstract：:Nucleotide excision repair (NER) mechanism is the major pathway responsible for the removal of a large variety of bulky lesions from the genome. Two different NER subpathways have been identified, i.e. the transcription-coupled and the global genome repair pathways. For DNA-damage induced by ultraviolet light both tra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.23.4653
更新日期：1996-12-01 00:00:00
abstract：:Modified bases, such as O6-methylguanines, are produced in cells exposed to alkylating agents and cause apoptosis. In human cells treated with N-methyl-N-nitrosourea, we detected a protein complex composed of MutSalpha, MutLalpha and PCNA on damaged DNA by immunoprecipitation method using chromatin extracts, in which ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki878
更新日期：2005-10-04 00:00:00
abstract：:Metagenomics has been employed to systematically sequence, classify, analyze and manipulate the entire genetic material isolated from environmental samples. Finding genes within metagenomic sequences remains a formidable challenge, and noncoding RNA genes other than those encoding rRNA and tRNA are not well annotated ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm057
更新日期：2007-01-01 00:00:00
abstract：:The rol-6 gene is trans-spliced to the 22 nt leader, SL1, 173 nt downstream of the transcription start. We have analyzed splicing in transformants carrying extrachromosomal arrays of rol-6 with mutations in the trans-splice acceptor site. This site is a close match to the consensus, UUUCAG, that is highly conserved in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.4.913
更新日期：1993-02-25 00:00:00
