Abstract:
:The genomic distribution of the abundant eukaryotic d(GA x TC)(n) DNA microsatellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombination in SV40 minichromosomes, the rate of homologous DNA recombination increasing by as much as two orders of magnitude in the presence of a d(GA x TC)(22) sequence. This effect depends on the region of the SV40 genome at which the d(GA x TC)(22) sequence is cloned. It is high when the sequence is located proximal to the SV40 control region but no effect is observed when located 3.5 kb away from the SV40 ori. These results indicate that the recombination potential of d(GA x TC)(n) sequences is likely linked to DNA replication and/or transcription. The potential contribution of the structural properties of d(GA x TC)(n) sequences to this effect is discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Benet A,Mollà G,Azorín Fdoi
10.1093/nar/28.23.4617keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
4617-22issue
23eissn
0305-1048issn
1362-4962journal_volume
28pub_type
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