Mouse genome database 2016.

abstract：:The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...

journal_title：Nucleic acids research

authors： Kumar G,Cuypers M,Webby RR,Webb TR,White SW

更新日期：2021-01-19 00:00:00

abstract：:An effective tool for the global analysis of both DNA methylation status and protein-chromatin interactions is a microarray constructed with sequences containing regulatory elements. One type of array suited for this purpose takes advantage of the strong association between CpG Islands (CGIs) and gene regulatory regio...

journal_title：Nucleic acids research

authors： Heisler LE,Torti D,Boutros PC,Watson J,Chan C,Winegarden N,Takahashi M,Yau P,Huang TH,Farnham PJ,Jurisica I,Woodgett JR,Bremner R,Penn LZ,Der SD

更新日期：2005-05-23 00:00:00

abstract：:The available reagents for the attachment of functional moieties to plasmid DNA are limiting. Most reagents bind plasmid DNA in a non-sequence- specific manner, with undefined stoichiometry, and affect DNA charge and delivery properties or involve chemical modifications that abolish gene expression. The design and abi...

journal_title：Nucleic acids research

authors： Hertoghs KM,Ellis JH,Catchpole IR

更新日期：2003-10-15 00:00:00

abstract：:A complete nucleotide sequence of human aldolase B mRNA was determined with a recombinant cDNA (pHABL120-3). The cDNA insert was composed of 1,652 bases excluding poly(A) tail and the sequence was consistent with the previous results reported by others. However, S1 nuclease mapping and subsequent genomic analysis allo...

journal_title：Nucleic acids research

authors： Sakakibara M,Mukai T,Yatsuki H,Hori K

更新日期：1985-07-25 00:00:00

abstract：:In metazoan cells, only a limited number of mini chromosome maintenance (MCM) complexes are fired during S phase, while the majority remain dormant. Several methods have been used to map replication origins, but such methods cannot identify dormant origins. Herein, we determined MCM7-binding sites in human cells using...

journal_title：Nucleic acids research

authors： Sugimoto N,Maehara K,Yoshida K,Ohkawa Y,Fujita M

更新日期：2018-07-27 00:00:00

abstract：:Computational analysis of biosynthetic gene clusters (BGCs) has revolutionized natural product discovery by enabling the rapid investigation of secondary metabolic potential within microbial genome sequences. Grouping homologous BGCs into Gene Cluster Families (GCFs) facilitates mapping their architectural and taxonom...

journal_title：Nucleic acids research

authors： Kautsar SA,Blin K,Shaw S,Weber T,Medema MH

更新日期：2021-01-08 00:00:00

abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...

journal_title：Nucleic acids research

authors： Li XM,Shapiro LJ

更新日期：1993-08-11 00:00:00

abstract：:A highly polymorphic locus associated with the variable tandem repetition of a 35 bp consensus sequence was mapped to chromosome 10, band q26. Examination of leukocyte DNA from a cancer patient revealed the twenty-fold amplification of one allelic fragment of this locus, while the other allelic fragment demonstrated a...

journal_title：Nucleic acids research

authors： Colb M,Yang-Feng T,Francke U,Mermer B,Parkinson DR,Krontiris TG

更新日期：1986-10-24 00:00:00

abstract：:The crystal structure of the RNA/DNA hybrid r(GAAGAGAAGC). d(GCTTCTCTTC) has been solved and refined at 2.5 A resolution. The refinement procedure converged at R = 0.181 for all reflections in the range 20.0-2.5 A. In the crystal, the RNA/DNA hybrid duplex has an A' conformation with all but one of the nucleotide suga...

journal_title：Nucleic acids research

authors： Conn GL,Brown T,Leonard GA

更新日期：1999-01-15 00:00:00

abstract：:Recently, prokaryotic riboswitches have been identified that regulate transcription in response to change of the concentration of secondary messengers. The ZMP (5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR))-sensing riboswitch from Thermosinus carboxydivorans is a transcriptional ON-switch that is involved in ...

journal_title：Nucleic acids research

authors： Binas O,Schamber T,Schwalbe H

更新日期：2020-07-09 00:00:00

abstract：:The essential and universal N(6)-threonylcarbamoyladenosine (t(6)A) modification at position 37 of ANN-decoding tRNAs plays a pivotal role in translational fidelity through enhancement of the cognate codon recognition and stabilization of the codon-anticodon interaction. In Escherichia coli, the YgjD (TsaD), YeaZ (Tsa...

journal_title：Nucleic acids research

authors： Zhang W,Collinet B,Perrochia L,Durand D,van Tilbeurgh H

更新日期：2015-02-18 00:00:00

abstract：:Next-generation DNA sequencing platforms provide exciting new possibilities for in vitro genetic analysis of functional nucleic acids. However, the size of the resulting data sets presents computational and analytical challenges. We present an open-source software package that employs a locality-sensitive hashing algo...

journal_title：Nucleic acids research

authors： Pitt JN,Rajapakse I,Ferré-D'Amaré AR

更新日期：2010-12-01 00:00:00

abstract：:Histone H2A ubiquitination plays critical roles in transcriptional repression and deoxyribonucleic acid (DNA) damage response. More attention has been focused on ubiquitin E3 ligases of H2A, however, less is known about the negative regulators of H2A ubiquitination. Here we identified HSCARG as a new negative regulato...

journal_title：Nucleic acids research

authors： Hu B,Li S,Zhang X,Zheng X

更新日期：2014-05-01 00:00:00

abstract：:We have developed a web-based resource (available at www.ciliate.org) for researchers studying the model ciliate organism Tetrahymena thermophila. Employing the underlying database structure and programming of the Saccharomyces Genome Database, the Tetrahymena Genome Database (TGD) integrates the wealth of knowledge g...

journal_title：Nucleic acids research

authors： Stover NA,Krieger CJ,Binkley G,Dong Q,Fisk DG,Nash R,Sethuraman A,Weng S,Cherry JM

更新日期：2006-01-01 00:00:00

abstract：:The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in...

journal_title：Nucleic acids research

authors： Hansen-Hagge TE,Trefzer U,zu Reventlow AS,Kaltoft K,Sterry W

更新日期：2001-02-15 00:00:00

abstract：:The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ε (Polε). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Polε. During efficient PCNA loading, CTF18-RFC ...

journal_title：Nucleic acids research

authors： Fujisawa R,Ohashi E,Hirota K,Tsurimoto T

更新日期：2017-05-05 00:00:00

abstract：:Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existence of a stable cross...

journal_title：Nucleic acids research

authors： Woppmann A,Rinke J,Lührmann R

更新日期：1988-12-09 00:00:00

abstract：:Defining syntenic relationships among orthologous gene clusters is a frequent undertaking of biologists studying organismal evolution through comparative genomic approaches. With the increasing availability of genome data made possible through next-generation sequencing technology, there is a growing need for user-fri...

journal_title：Nucleic acids research

authors： Veltri D,Wight MM,Crouch JA

更新日期：2016-07-08 00:00:00

abstract：:Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...

journal_title：Nucleic acids research

authors： Ferrando A,Koncz-Kálmán Z,Farràs R,Tiburcio A,Schell J,Koncz C

更新日期：2001-09-01 00:00:00

abstract：:Structured RNA molecules form complex 3D architectures stabilized by multiple interactions involving the nucleotide base, sugar and phosphate moieties. A significant percentage of the bases in structured RNA molecules in the Protein Data Bank (PDB) hydrogen-bond with phosphates of other nucleotides. By extracting and ...

journal_title：Nucleic acids research

authors： Zirbel CL,Sponer JE,Sponer J,Stombaugh J,Leontis NB

更新日期：2009-08-01 00:00:00

abstract：:A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields. ...

journal_title：Nucleic acids research

authors： Gait MJ,Singh M,Sheppard RC,Edge MD,Greene AR,Heathcliffe GR,Atkinson TC,Newton CR,Markham AF

更新日期：1980-03-11 00:00:00

abstract：:The Protein Folding Database (PFD) is a publicly accessible repository of thermodynamic and kinetic protein folding data. Here we describe the first major revision of this work, featuring extensive restructuring that conforms to standards set out by the recently formed International Foldeomics Consortium. The database...

journal_title：Nucleic acids research

authors： Fulton KF,Bate MA,Faux NG,Mahmood K,Betts C,Buckle AM

更新日期：2007-01-01 00:00:00

abstract：:Two factors are mainly responsible for the stability of the DNA double helix: base pairing between complementary strands and stacking between adjacent bases. By studying DNA molecules with solitary nicks and gaps we measure temperature and salt dependence of the stacking free energy of the DNA double helix. For the fi...

journal_title：Nucleic acids research

authors： Yakovchuk P,Protozanova E,Frank-Kamenetskii MD

更新日期：2006-01-31 00:00:00

abstract：:Two similar, but not identical, models have been proposed for the amino acid-base pair contacts in the CAP-DNA complex ('Model I,' Weber, I. and Steitz, T., Proc. Natl. Acad. Sci. USA, 81, 3973-3977, 1984; 'Model II,' Ebright, et al., Proc. Natl. Acad. Sci. USA, 81, 7274-7278, 1984). One difference between the two mod...

journal_title：Nucleic acids research

authors： Gunasekera A,Ebright YW,Ebright RH

更新日期：1990-12-11 00:00:00

abstract：:Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and...

journal_title：Nucleic acids research

authors： Finn PJ,Gibson NJ,Fallon R,Hamilton A,Brown T

更新日期：1996-09-01 00:00:00

abstract：:A 170bp long BamHI-Sau3A DNA fragment from the actIII-actI intergenic region of the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2) contains two promoters directing transcription in a divergent manner. One of them, the actIII promoter, is responsible for the transcription of the actIII ge...

journal_title：Nucleic acids research

authors： Parro V,Hopwood DA,Malpartida F,Mellado RP

更新日期：1991-05-25 00:00:00

abstract：:Single-stranded guanosine-rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G-quartets. In addition to intense speculation about the role of G-quartet formation in vivo, there is considerable interest in the therapeutic potential of quadruplex oligonucleotides...

journal_title：Nucleic acids research

authors： Dapić V,Abdomerović V,Marrington R,Peberdy J,Rodger A,Trent JO,Bates PJ

更新日期：2003-04-15 00:00:00

abstract：:The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1976-12-01 00:00:00

abstract：:Reactome (http://www.reactome.org) is a manually curated open-source open-data resource of human pathways and reactions. The current version 46 describes 7088 human proteins (34% of the predicted human proteome), participating in 6744 reactions based on data extracted from 15 107 research publications with PubMed link...

journal_title：Nucleic acids research

authors： Croft D,Mundo AF,Haw R,Milacic M,Weiser J,Wu G,Caudy M,Garapati P,Gillespie M,Kamdar MR,Jassal B,Jupe S,Matthews L,May B,Palatnik S,Rothfels K,Shamovsky V,Song H,Williams M,Birney E,Hermjakob H,Stein L,D'Eusta

更新日期：2014-01-01 00:00:00

abstract：:The crystal structure of the DNA dodecamer duplex CATGGGCCCATG lies on a structural continuum along the transition between A- and B-DNA. The dodecamer possesses the normal vector plot and inclination values typical of B-DNA, but has the crystal packing, helical twist, groove width, sugar pucker, slide and x-displaceme...

journal_title：Nucleic acids research

authors： Ng HL,Dickerson RE

更新日期：2002-09-15 00:00:00