Three-step PCR mutagenesis for 'linker scanning'.

abstract：:Fluorogenic RNAs that are based on the complex formed by 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) derivatives and the RNA aptamer named Spinach were used to engineer a new generation of in vitro and in vivo sensors for bioanalytics. With the resolved crystal structure of the RNA/small molecule complex, ...

journal_title：Nucleic acids research

authors： Ketterer S,Fuchs D,Weber W,Meier M

更新日期：2015-10-30 00:00:00

abstract：:The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment whi...

journal_title：Nucleic acids research

authors： Pannekoek H,Maat J,van den Berg E,Noordermeer I

更新日期：1980-04-11 00:00:00

abstract：:Cloned coding sequences for rat and human ornithine transcarbamylase (OTC) were obtained by screening a rat and a human cDNA library respectively with a synthetic oligonucleotide corresponding to 27 bases of the rat sequence. These clones, 1100 bp long for the rat clone and 1300 bp for the human, contain approximately...

journal_title：Nucleic acids research

authors： Davies KE,Briand P,Ionasescu V,Ionasescu G,Williamson R,Brown C,Cavard C,Cathelineau L

更新日期：1985-01-11 00:00:00

abstract：:Complex carbohydrates of plants are the main food sources of animals and microbes, and serve as promising renewable feedstock for biofuel and biomaterial production. Carbohydrate active enzymes (CAZymes) are the most important enzymes for complex carbohydrate metabolism. With an increasing number of plant and plant-as...

journal_title：Nucleic acids research

authors： Zhang H,Yohe T,Huang L,Entwistle S,Wu P,Yang Z,Busk PK,Xu Y,Yin Y

更新日期：2018-07-02 00:00:00

abstract：:Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog s...

journal_title：Nucleic acids research

authors： Miskey C,Izsvák Z,Plasterk RH,Ivics Z

更新日期：2003-12-01 00:00:00

abstract：:Over millions of years the structure and complexity of the transcriptional regulatory network (TRN) in bacteria has changed, reorganized and enabled them to adapt to almost every environmental niche on earth. In order to understand the plasticity of TRNs in bacteria, we studied the conservation of currently known TRNs...

journal_title：Nucleic acids research

authors： Lozada-Chávez I,Janga SC,Collado-Vides J

更新日期：2006-07-13 00:00:00

abstract：:RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated RNAs. The protein's non-catalytic C-...

journal_title：Nucleic acids research

authors： Ali N,Gowrishankar J

更新日期：2020-01-24 00:00:00

abstract：:We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...

journal_title：Nucleic acids research

authors： Potrykus K,Murphy H,Chen X,Epstein JA,Cashel M

更新日期：2010-03-01 00:00:00

abstract：:PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes s...

journal_title：Nucleic acids research

authors： Dutreix M,Bäckman A,Célérier J,Bagdasarian MM,Sommer S,Bailone A,Devoret R,Bagdasarian M

更新日期：1988-11-25 00:00:00

abstract：:Linear motifs are short, evolutionarily plastic components of regulatory proteins and provide low-affinity interaction interfaces. These compact modules play central roles in mediating every aspect of the regulatory functionality of the cell. They are particularly prominent in mediating cell signaling, controlling pro...

journal_title：Nucleic acids research

authors： Dinkel H,Michael S,Weatheritt RJ,Davey NE,Van Roey K,Altenberg B,Toedt G,Uyar B,Seiler M,Budd A,Jödicke L,Dammert MA,Schroeter C,Hammer M,Schmidt T,Jehl P,McGuigan C,Dymecka M,Chica C,Luck K,Via A,Chatr-Aryamont

更新日期：2012-01-01 00:00:00

abstract：:We have examined the formation of alternate strand triple-helices at the target sequence A11(TC)6.(GA)6T11 using the oligonucleotides T11(AG)6 and T11(TG)6, by DNase I footprinting. These third strands were designed so as to form parallel T.AT triplets together with antiparallel G.GC and A.AT or T.AT triplets. We find...

journal_title：Nucleic acids research

authors： Washbrook E,Fox KR

更新日期：1994-09-25 00:00:00

abstract：:Rad51 protein promotes homologous recombination in eukaryotes. Recombination activities are activated by Rad51 filament assembly on ssDNA. Previous studies of yeast Rad51 showed that His352 occupies an important position at the filament interface, where it could relay signals between subunits and active sites. To inve...

journal_title：Nucleic acids research

authors： Chen J,Villanueva N,Rould MA,Morrical SW

更新日期：2010-08-01 00:00:00

abstract：:Mutations help us to understand the molecular origins of diseases. Researchers, therefore, both publish and seek disease-relevant mutations in public databases and in scientific literature, e.g. Medline. The retrieval tends to be time-consuming and incomplete. Automated screening of the literature is more efficient. W...

journal_title：Nucleic acids research

authors： Rebholz-Schuhmann D,Marcel S,Albert S,Tolle R,Casari G,Kirsch H

更新日期：2004-01-02 00:00:00

abstract：:Many tools are available to analyse genomes but are often challenging to use in a cell type-specific context. We have developed a method similar to the isolation of nuclei tagged in a specific cell type (INTACT) technique [Deal,R.B. and Henikoff,S. (2010) A simple method for gene expression and chromatin profiling of ...

journal_title：Nucleic acids research

authors： Henry GL,Davis FP,Picard S,Eddy SR

更新日期：2012-10-01 00:00:00

abstract：:A circular denaturation and restriction map of mitochondrial DNA from Neurospora crassa is presented. The map shows the position of all twelve fragments produced by restriction endonuclease Eco R I and the position of the largest Hin III fragment along the previously established map of AT-rich sequences. The two wild ...

journal_title：Nucleic acids research

authors： Bernard U,Goldthwaite C,Küntzel H

更新日期：1976-11-01 00:00:00

abstract：:The non-essential MGS1 gene of Saccharomyces cerevisiae is highly conserved in eukaryotes and encodes an enzyme containing both DNA-dependent ATPase and DNA annealing activities. MGS1 appears to function in post-replicational repair processes that contribute to genome stability. In this study, we identified MGS1 as a ...

journal_title：Nucleic acids research

authors： Kim JH,Kang YH,Kang HJ,Kim DH,Ryu GH,Kang MJ,Seo YS

更新日期：2005-10-26 00:00:00

abstract：:The bacteriophage ø29 DNA packaging motor that assembles on the precursor capsid (prohead) contains an essential 174-nt structural RNA (pRNA) that forms multimers. To determine the structural features of the CE- and D-loops believed to be involved in multimerization of pRNA, 35- and 19-nt RNA molecules containing the ...

journal_title：Nucleic acids research

authors： Kitamura A,Jardine PJ,Anderson DL,Grimes S,Matsuo H

更新日期：2008-02-01 00:00:00

abstract：:The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for ...

journal_title：Nucleic acids research

authors： Guilley H,Jonard G,Kukla B,Richards KE

更新日期：1979-04-01 00:00:00

abstract：:Oligoamines (spermidine, dipropylenetriamine and propylenediamine) were covalently attached to acridine via a hexamethylene linker. These oligoamine-acridine conjugates were efficiently bound to gap sites in substrate DNA, and promoted the DNA hydrolysis by a homogeneous Ce(IV)/ethylenediamine-N,N,N',N'-tetraacetate (...

journal_title：Nucleic acids research

authors： Yamamoto Y,Tsuboi W,Komiyama M

更新日期：2003-08-01 00:00:00

abstract：:Genome-wide association studies (GWAS) have become a preferred method to identify new genetic susceptibility loci. This technique aims to understanding the molecular etiology of common diseases, but in many cases, it has led to the identification of loci with no obvious biological relevance. Herein, we show that previ...

journal_title：Nucleic acids research

authors： Galichon P,Mesnard L,Hertig A,Stengel B,Rondeau E

更新日期：2012-06-01 00:00:00

abstract：T:Activator protein 1 (AP-1) is a transcription factor that recognizes two versions of a 7-base pair response element, either 5΄- GAG CA-3΄ or 5΄- GAG CA-3΄ (where M = 5-methylcytosine). These two elements share the feature that 5-methylcytosine and thymine both have a methyl group in the same position, 5-carbon of the...

journal_title：Nucleic acids research

authors： Hong S,Wang D,Horton JR,Zhang X,Speck SH,Blumenthal RM,Cheng X

更新日期：2017-03-17 00:00:00

abstract：:The restriction endonuclease (R.BsuFI) of Bacillus subtilis recognizes the target DNA sequence 5' CCGG. The R.BsuFI gene was found in close proximity to the cognate M.BsuFI gene, which had previously been characterized (1). Cloning of the R.BsuFI gene in E.coli was only possible with the M.BsuFI Mtase gene present on ...

journal_title：Nucleic acids research

authors： Kapfer W,Walter J,Trautner TA

更新日期：1991-12-11 00:00:00

abstract：:Circular DNA aptamers are powerful candidates for therapeutic applications given their dramatically enhanced biostability. Herein we report the first effort to evolve circular DNA aptamers that bind a human protein directly in serum, a complex biofluid. Targeting human thrombin, this strategy has led to the discovery ...

journal_title：Nucleic acids research

authors： Mao Y,Gu J,Chang D,Wang L,Yao L,Ma Q,Luo Z,Qu H,Li Y,Zheng L

更新日期：2020-11-04 00:00:00

abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

journal_title：Nucleic acids research

authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

更新日期：2014-04-01 00:00:00

abstract：:Improving the accuracy of prediction of gene starts is one of a few remaining open problems in computer prediction of prokaryotic genes. Its difficulty is caused by the absence of relatively strong sequence patterns identifying true translation initiation sites. In the current paper we show that the accuracy of gene s...

journal_title：Nucleic acids research

authors： Besemer J,Lomsadze A,Borodovsky M

更新日期：2001-06-15 00:00:00

abstract：:The Mouse Genome Database (MGD) forms the core of the Mouse Genome Informatics (MGI) system (http://www.informatics.jax.org), a model organism database resource for the laboratory mouse. MGD provides essential integration of experimental knowledge for the mouse system with information annotated from both literature an...

journal_title：Nucleic acids research

authors： Eppig JT,Bult CJ,Kadin JA,Richardson JE,Blake JA,Anagnostopoulos A,Baldarelli RM,Baya M,Beal JS,Bello SM,Boddy WJ,Bradt DW,Burkart DL,Butler NE,Campbell J,Cassell MA,Corbani LE,Cousins SL,Dahmen DJ,Dene H,Diehl AD

更新日期：2005-01-01 00:00:00

abstract：:Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Ini...

journal_title：Nucleic acids research

authors： Zhang ZY,Thompson EA,Stallcup MR

更新日期：1984-11-12 00:00:00

abstract：:Structural domains are considered as the basic units of protein folding, evolution, function and design. Automatic decomposition of protein structures into structural domains, though after many years of investigation, remains a challenging and unsolved problem. Manual inspection still plays a key role in domain decomp...

journal_title：Nucleic acids research

authors： Guo JT,Xu D,Kim D,Xu Y

更新日期：2003-02-01 00:00:00

abstract：:The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs...

journal_title：Nucleic acids research

authors： Araco A,Belli M,Giorgi C,Onori G

更新日期：1975-03-01 00:00:00

abstract：:Recently, we developed a mass DNA-mediated transformation technique for the ciliated protozoan Tetrahymena thermophila that introduces transforming DNA by electroporation into conjugating cells. Other studies demonstrated that a neomycin resistance gene flanked by Tetrahymena H4-I gene regulatory sequences transformed...

journal_title：Nucleic acids research

authors： Gaertig J,Gu L,Hai B,Gorovsky MA

更新日期：1994-12-11 00:00:00