Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5.


:PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes share extensive homology the predicted amino acid sequences of the two proteins differing by 5 residues, (ii) the expression of R6-5 psiB is 4 times higher than F psiB gene, (iii) in plasmid R6-5, a Tn10 transposon upstream from the psiB gene enhances psiB expression. Hence, the F sex factor may be unable to prevent SOS induction for two non-exclusive reasons: (i) F PsiB protein, being slightly different from R6-5, may be less active, (ii) the level of synthesis of F PsiB protein may be insufficient to prevent SOS induction.


Nucleic Acids Res


Nucleic acids research


Dutreix M,Bäckman A,Célérier J,Bagdasarian MM,Sommer S,Bailone A,Devoret R,Bagdasarian M




Has Abstract


1988-11-25 00:00:00












  • Dual transcriptional-translational cascade permits cellular level tuneable expression control.

    abstract::The ability to induce gene expression in a small molecule dependent manner has led to many applications in target discovery, functional elucidation and bio-production. To date these applications have relied on a limited set of protein-based control mechanisms operating at the level of transcription initiation. The dis...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Morra R,Shankar J,Robinson CJ,Halliwell S,Butler L,Upton M,Hay S,Micklefield J,Dixon N

    更新日期:2016-02-18 00:00:00

  • A short 5' region of the long terminal repeat is required for regulation by hormone and heat shock of Drosophila retrotransposon 1731.

    abstract::1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or de...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ziarczyk P,Best-Belpomme M

    更新日期:1991-10-25 00:00:00

  • Biocuration of functional annotation at the European nucleotide archive.

    abstract::The European Nucleotide Archive (ENA; is a repository for the submission, maintenance and presentation of nucleotide sequence data and related sample and experimental information. In this article we report on ENA in 2015 regarding general activity, notable published data sets and major achiev...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gibson R,Alako B,Amid C,Cerdeño-Tárraga A,Cleland I,Goodgame N,Ten Hoopen P,Jayathilaka S,Kay S,Leinonen R,Liu X,Pallreddy S,Pakseresht N,Rajan J,Rosselló M,Silvester N,Smirnov D,Toribio AL,Vaughan D,Zalunin V,Coc

    更新日期:2016-01-04 00:00:00

  • Molecular Biology Database List.

    abstract::Molecular Biology Database List (MBDL) includes brief descriptions and pointers to Web sites for the various databases described in this issue as well as other Web sites presenting data sets relevant to molecular biology. This information is compiled into a list ( gk...

    journal_title:Nucleic acids research



    authors: Burks C

    更新日期:1999-01-01 00:00:00

  • The inhibition of mitochondrial DNA polymerase gamma from animal cells by intercalating drugs.

    abstract::DNA polymerase gamma from purified nuclei of EMT-6 cells (mice) seems to be identical to the mitochondrial DNA polymerase from the same source following several criteria. These two enzyme activities are strongly inhibited by ethidium bromide and acriflavin, while proflavin, acridine orange, daunomycin and chloroquine ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tarrago-Litvak L,Viratelle O,Darriet D,Dalibart R,Graves PV,Litvak S

    更新日期:1978-06-01 00:00:00

  • The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach.

    abstract::The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Araco A,Belli M,Giorgi C,Onori G

    更新日期:1975-03-01 00:00:00

  • CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance.

    abstract::To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Feng X,Hsu SJ,Kasbek C,Chaiken M,Price CM

    更新日期:2017-05-05 00:00:00

  • An error-prone family Y DNA polymerase (DinB homolog from Sulfolobus solfataricus) uses a 'steric gate' residue for discrimination against ribonucleotides.

    abstract::DNA polymerases of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl group on the incoming nucleotide. In the family Y (lesion bypass) polyme...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: DeLucia AM,Grindley ND,Joyce CM

    更新日期:2003-07-15 00:00:00

  • Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation.

    abstract::CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA-aided self-cleavag...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Shen CC,Hsu MN,Chang CW,Lin MW,Hwu JR,Tu Y,Hu YC

    更新日期:2019-02-20 00:00:00

  • Role of RNA structure in non-homologous recombination between genomic molecules of brome mosaic virus.

    abstract::Brome mosaic virus (BMV) is a tripartite genome, positive-sense RNA virus of plants. Previously it was demonstrated that local hybridization between BMV RNAs (RNA-RNA heteroduplex formation) efficiently promotes non-homologous RNA recombination. In addition, studies on the role of the BMV polymerase in RNA recombinati...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Figlerowicz M

    更新日期:2000-04-15 00:00:00

  • A novel mechanism of selectivity against AZT by the human mitochondrial DNA polymerase.

    abstract::Native nucleotides show a hyperbolic concentration dependence of the pre-steady-state rate of incorporation while maintaining concentration-independent amplitude due to fast, largely irreversible pyrophosphate release. The kinetics of 3'-azido-2',3'-dideoxythymidine (AZT) incorporation exhibit an increase in amplitude...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hanes JW,Johnson KA

    更新日期:2007-01-01 00:00:00

  • Transcription frequency modulates the efficiency of an attenuator preceding the rpoBC RNA polymerase genes of Escherichia coli: possible autogenous control.

    abstract::Expression of the rpoBC genes encoding the beta and beta' RNA polymerase subunits of Escherichia coli is autogenously regulated. Although previous studies have demonstrated a post-transcriptional feedback mechanism, complex transcriptional controls of rpoBC expression may also contribute. We show that an attenuator (r...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Steward KL,Linn T

    更新日期:1992-09-25 00:00:00

  • A one-step method for quantitative determination of uracil in DNA by real-time PCR.

    abstract::Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The archaeal DNA polyme...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Horváth A,Vértessy BG

    更新日期:2010-11-01 00:00:00

  • RORα switches transcriptional mode of ERRγ that results in transcriptional repression of CYP2E1 under ethanol-exposure.

    abstract::Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Han YH,Kim DK,Na TY,Ka NL,Choi HS,Lee MO

    更新日期:2016-02-18 00:00:00

  • Asn-tRNA in Lactobacillus bulgaricus is formed by asparaginylation of tRNA and not by transamidation of Asp-tRNA.

    abstract::In many organisms (e.g., gram-positive eubacteria) Gin-tRNA is not formed by direct glutaminylation of tRNAGln but by a specific transamidation of Glu-tRNAGln. We wondered whether a similar transamidation pathway also operates in the formation of Asn-tRNA in these organisms. Therefore we tested in S-100 preparations o...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kim SI,Nalaskowska M,Germond JE,Pridmore D,Söll D

    更新日期:1996-07-15 00:00:00

  • SimpleSynteny: a web-based tool for visualization of microsynteny across multiple species.

    abstract::Defining syntenic relationships among orthologous gene clusters is a frequent undertaking of biologists studying organismal evolution through comparative genomic approaches. With the increasing availability of genome data made possible through next-generation sequencing technology, there is a growing need for user-fri...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Veltri D,Wight MM,Crouch JA

    更新日期:2016-07-08 00:00:00

  • PSSweb: protein structural statistics web server.

    abstract::With the increasing number of protein structures available, there is a need for tools capable of automating the comparison of ensembles of structures, a common requirement in structural biology and bioinformatics. PSSweb is a web server for protein structural statistics. It takes as input an ensemble of PDB files of p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gaillard T,Stote RH,Dejaegere A

    更新日期:2016-07-08 00:00:00

  • DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system.

    abstract::Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fedorova I,Arseniev A,Selkova P,Pobegalov G,Goryanin I,Vasileva A,Musharova O,Abramova M,Kazalov M,Zyubko T,Artamonova T,Artamonova D,Shmakov S,Khodorkovskii M,Severinov K

    更新日期:2020-02-28 00:00:00

  • Detection and identification of minor nucleotides in intact deoxyribonucleic acids by mass spectrometry.

    abstract::A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wiebers JL

    更新日期:1976-11-01 00:00:00

  • DNA multiplex hybridization on microarrays and thermodynamic stability in solution: a direct comparison.

    abstract::Hybridization intensities of 30 distinct short duplex DNAs measured on spotted microarrays, were directly compared with thermodynamic stabilities measured in solution. DNA sequences were designed to promote formation of perfect match, or hybrid duplexes containing tandem mismatches. Thermodynamic parameters DeltaH deg...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fish DJ,Horne MT,Brewood GP,Goodarzi JP,Alemayehu S,Bhandiwad A,Searles RP,Benight AS

    更新日期:2007-01-01 00:00:00

  • A cytoplasm-specific activity encoded by the Trithorax-like ATX1 gene.

    abstract::Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ndamukong I,Lapko H,Cerny RL,Avramova Z

    更新日期:2011-06-01 00:00:00

  • Retroposons do jump: a B2 element recently integrated in an 18S rDNA gene.

    abstract::Several cDNA clones were isolated from cDNA libraries constructed with mRNA longer than 28S RNA from the murine cell line PYS-2/12. The plasmids have inserts containing 1-1.2 kb of the ribosomal 5' external transcribed spacer followed by nearly 700 nt of sequence for 18S rRNA and ending with a B2 element (retroposon)....

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Oberbäumer I

    更新日期:1992-02-25 00:00:00

  • Conditional DNA repair mutants enable highly precise genome engineering.

    abstract::Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nyerges Á,Csorgő B,Nagy I,Latinovics D,Szamecz B,Pósfai G,Pál C

    更新日期:2014-04-01 00:00:00

  • Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.

    abstract::As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the fam...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Massad T,Skaar K,Nilsson H,Damberg P,Henriksson-Peltola P,Haggård-Ljungquist E,Högbom M,Stenmark P

    更新日期:2010-11-01 00:00:00

  • Molecular characterization at the RNA and gene levels of U3 snoRNA from a unicellular green alga, Chlamydomonas reinhardtii.

    abstract::A U3 snoRNA gene isolated from a Chlamydomonas reinhardtii (CRE:) genomic library contains putative pol III-specific transcription signals similar to those of RNA polymerase III-specific small nuclear (sn)RNA genes of higher plants. The 222 nt long CRE: U3 snoRNA was immunoprecipitated by anti-gamma-mpppN antisera, bu...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Antal M,Mougin A,Kis M,Boros E,Steger G,Jakab G,Solymosy F,Branlant C

    更新日期:2000-08-01 00:00:00

  • GenBank.

    abstract::GenBank (R) is a comprehensive sequence database that contains publicly available DNA sequences for more than 119 000 different organisms, obtained primarily through the submission of sequence data from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Benson DA,Karsch-Mizrachi I,Lipman DJ,Ostell J,Wheeler DL

    更新日期:2003-01-01 00:00:00

  • Structural distinctions between NAD+ riboswitch domains 1 and 2 determine differential folding and ligand binding.

    abstract::Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises t...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chen H,Egger M,Xu X,Flemmich L,Krasheninina O,Sun A,Micura R,Ren A

    更新日期:2020-12-02 00:00:00

  • Site-specific DNA binding of nuclear factor I: effect of the spacer region.

    abstract::Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus type 2 DNA in vitro and in vivo. To study sequence requirements for the interaction of NFI with DNA, we have measured the binding of the protein to a variety of synthetic sites. Binding sites for NFI (FIB sites) we...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gronostajski RM

    更新日期:1987-07-24 00:00:00

  • Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells.

    abstract::The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Fütterer J,Hohn T

    更新日期:1992-08-11 00:00:00

  • The MRE11-NBS1-RAD50 pathway is perturbed in SV40 large T antigen-immortalized AT-1, AT-2 and HL-1 cardiomyocytes.

    abstract::To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and micro-sequen...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lanson NA Jr,Egeland DB,Royals BA,Claycomb WC

    更新日期:2000-08-01 00:00:00