Abstract:
:PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes share extensive homology the predicted amino acid sequences of the two proteins differing by 5 residues, (ii) the expression of R6-5 psiB is 4 times higher than F psiB gene, (iii) in plasmid R6-5, a Tn10 transposon upstream from the psiB gene enhances psiB expression. Hence, the F sex factor may be unable to prevent SOS induction for two non-exclusive reasons: (i) F PsiB protein, being slightly different from R6-5, may be less active, (ii) the level of synthesis of F PsiB protein may be insufficient to prevent SOS induction.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Dutreix M,Bäckman A,Célérier J,Bagdasarian MM,Sommer S,Bailone A,Devoret R,Bagdasarian Mdoi
10.1093/nar/16.22.10669subject
Has Abstractpub_date
1988-11-25 00:00:00pages
10669-79issue
22eissn
0305-1048issn
1362-4962journal_volume
16pub_type
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