Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant.

abstract：:We have constructed a complete, five-enzyme restriction map of the genome of the archaeon Halobacterium sp. GRB, based on a set of 84 overlapping cosmid clones. Fewer than 30 kbp, in three gaps, remain uncloned. The genome consists of five replicons: a chromosome (2038 kbp) and four plasmids (305, 90, 37, and 1.8 kbp)...

journal_title：Nucleic acids research

authors： St Jean A,Trieselmann BA,Charlebois RL

更新日期：1994-04-25 00:00:00

abstract：:The antigene strategy based on site-specific recognition of duplex DNA by triplex DNA formation has been exploited in a wide range of biological activities. However, specific triplex formation is mostly restricted to homo-purine strands within the target duplex DNA, due to the destabilizing effect of CG and TA inversi...

journal_title：Nucleic acids research

authors： Wang L,Taniguchi Y,Okamura H,Sasaki S

更新日期：2018-09-28 00:00:00

abstract：:Gel migration and uranyl photoprobing have been used to study the effects of inosine and 2,6-diaminopurine (2,6-DAP) substitution on adenine-tract (A-tract) induced DNA curvature. Using a 10mer repeated sequence including five inosines we show by uranyl photoprobing that a narrow minor groove varying in phase with the...

journal_title：Nucleic acids research

authors： Mollegaard NE,Bailly C,Waring MJ,Nielsen PE

更新日期：1997-09-01 00:00:00

abstract：:The comprehensive multiplatform genomics data generated by The Cancer Genome Atlas (TCGA) Research Network is an enabling resource for cancer research. It includes an unprecedented amount of microRNA sequence data: ~11 000 libraries across 33 cancer types. Combined with initiatives like the National Cancer Institute G...

journal_title：Nucleic acids research

authors： Chu A,Robertson G,Brooks D,Mungall AJ,Birol I,Coope R,Ma Y,Jones S,Marra MA

更新日期：2016-01-08 00:00:00

abstract：:Here, we report a webserver for the improved SDM, used for predicting the effects of mutations on protein stability. As a pioneering knowledge-based approach, SDM has been highlighted as the most appropriate method to use in combination with many other approaches. We have updated the environment-specific amino-acid su...

journal_title：Nucleic acids research

authors： Pandurangan AP,Ochoa-Montaño B,Ascher DB,Blundell TL

更新日期：2017-07-03 00:00:00

abstract：:The compilation of 5S rRNA and 5S rRNA gene nucleotide sequences as of 30 September 1996, contains a total of 1661 primary structures of 5S rRNAs or their genes, which is an increase of 928 new sequence entries over the last compilation. It covers sequences from 54 archaea, 449 eubacteria, 34 plastids, nine mitochondr...

journal_title：Nucleic acids research

authors： Specht T,Szymanski M,Barciszewska MZ,Barciszewski J,Erdmann VA

更新日期：1997-01-01 00:00:00

abstract：:The mutations of 76 haemophilia B patients representing the whole population registered with the Malmö haemophilia centre (42) and referrals from the UK, were characterised. RFLP haplotype analysis of the defective genes indicated that 51 single base pair substitutions were definitely of independent origin and 27 of t...

journal_title：Nucleic acids research

authors： Green PM,Montandon AJ,Bentley DR,Ljung R,Nilsson IM,Giannelli F

更新日期：1990-06-11 00:00:00

abstract：:Short interfering RNAs (siRNAs) are valuable reagents for sequence-specific inhibition of gene expression via the RNA interference (RNAi) pathway. Although it has been proposed that the relative thermodynamic stability at the 5'-ends of siRNAs plays a crucial role in siRNA strand selection, we demonstrate here that a ...

journal_title：Nucleic acids research

authors： Sano M,Sierant M,Miyagishi M,Nakanishi M,Takagi Y,Sutou S

更新日期：2008-10-01 00:00:00

abstract：:A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...

journal_title：Nucleic acids research

authors： Lemp NA,Hiraoka K,Kasahara N,Logg CR

更新日期：2012-08-01 00:00:00

abstract：:As shown in the accompanying paper (5), the oligonucleotide composition of the E. coli genome is highly asymmetric for sequences up to 6 bp in length when ranked from highest to lowest abundance. We show here that this largely reflects codon usage because heavily used codons were found in the highly abundant oligomers...

journal_title：Nucleic acids research

authors： Phillips GJ,Arnold J,Ivarie R

更新日期：1987-03-25 00:00:00

abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...

journal_title：Nucleic acids research

authors： Flybase Consortium.

更新日期：1996-01-01 00:00:00

abstract：:Intestinal apolipoprotein B mRNA is edited at nucleotide 6666 by a C to U transition resulting in a translational stop codon. The enzymatic properties of the editing activity were characterised in vitro using rat enterocyte cytosolic extract. The editing activity has no nucleotide or ion cofactor requirement. It shows...

journal_title：Nucleic acids research

authors： Greeve J,Navaratnam N,Scott J

更新日期：1991-07-11 00:00:00

abstract：:Electrospray mass spectrometry was evaluated regarding the reliability of the determination of the stoichiometries and equilibrium association constants from single spectra. Complexes between minor groove binders (Hoechst 33258, Hoechst 33342, DAPI, netropsin and berenil) and 12mer oligonucleotide duplexes with a cent...

journal_title：Nucleic acids research

authors： Rosu F,Gabelica V,Houssier C,De Pauw E

更新日期：2002-08-15 00:00:00

abstract：:Predictably regulating protein expression levels to improve recombinant protein production has become an important tool, but is still rarely applied to engineer mammalian cells. We therefore sought to set-up an easy-to-implement toolbox to facilitate fast and reliable regulation of protein expression in mammalian cell...

journal_title：Nucleic acids research

authors： Eisenhut P,Mebrahtu A,Moradi Barzadd M,Thalén N,Klanert G,Weinguny M,Sandegren A,Su C,Hatton D,Borth N,Rockberg J

更新日期：2020-11-18 00:00:00

abstract：:It is well established that gene regulation can be achieved through activator and repressor proteins that bind to DNA and switch particular genes on or off, and that complex metabolic networks determine the levels of transcription of a given gene at a given time. Using three complementary computational techniques to s...

journal_title：Nucleic acids research

authors： Sutthibutpong T,Matek C,Benham C,Slade GG,Noy A,Laughton C,K Doye JP,Louis AA,Harris SA

更新日期：2016-11-02 00:00:00

abstract：:We show that nucleic acid structures may be conveniently and inexpensively characterized by their UV thermal difference spectra. A thermal difference spectrum (TDS) is obtained for a nucleic acid by simply recording the ultraviolet absorbance spectra of the unfolded and folded states at temperatures above and below it...

journal_title：Nucleic acids research

authors： Mergny JL,Li J,Lacroix L,Amrane S,Chaires JB

更新日期：2005-09-12 00:00:00

abstract：:We have investigated the substrate specificity of the Ogg1 protein of Saccharomyces cerevisiae (yOgg1 protein) for excision of modified DNA bases from oxidatively damaged DNA substrates using gas chromatography/isotope dilution mass spectrometry. Four DNA substrates prepared by treatment with H2O2/Fe(III)-EDTA/ascorbi...

journal_title：Nucleic acids research

authors： Karahalil B,Girard PM,Boiteux S,Dizdaroglu M

更新日期：1998-03-01 00:00:00

abstract：:The nucleotide sequence of the chick beta-actin gene was determined. The gene contains 5 introns; 4 interrupt the translated region at codons 41/42, 120/122, 267, 327/328 and a large intron occurs in the 5' untranslated region. The gene has a 97 nucleotide 5'-untranslated region and a 594 nucleotide 3'-untranslated re...

journal_title：Nucleic acids research

authors： Kost TA,Theodorakis N,Hughes SH

更新日期：1983-12-10 00:00:00

abstract：:One of the master regulators of placental cell fusion in mammals leading to multi-nucleated syncytiotrophoblasts is the transcription factor GCMa. Recently, we proved that the cAMP-driven protein kinase A signaling pathway is fundamental for up-regulation of GCMa transcript levels and protein stability. Here, we show ...

journal_title：Nucleic acids research

authors： Schubert SW,Abendroth A,Kilian K,Vogler T,Mayr B,Knerr I,Hashemolhosseini S

更新日期：2008-06-01 00:00:00

abstract：:During DNA double-strand break and replication fork repair by homologous recombination, the RAD51 recombinase catalyzes the DNA strand exchange reaction via a helical polymer assembled on single-stranded DNA, termed the presynaptic filament. Our published work has demonstrated a dual function of the SWI5-SFR1 complex ...

journal_title：Nucleic acids research

authors： Su GC,Yeh HY,Lin SW,Chung CI,Huang YS,Liu YC,Lyu PC,Chi P

更新日期：2016-07-27 00:00:00

abstract：:A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents...

journal_title：Nucleic acids research

authors： Patel TP,Millican TA,Bose CC,Titmas RC,Mock GA,Eaton MA

更新日期：1982-09-25 00:00:00

abstract：:Here we compare several methods for predicting oligonucleotide frequencies in 691 kb of Drosophila melanogaster DNA. As in previous work on Escherichia coli and Saccharomyces cerevisiae, a relatively simple equation based on tetranucleotide frequencies can be used in predicting frequencies of higher order oligonucleot...

journal_title：Nucleic acids research

authors： Cuticchia AJ,Ivarie R,Arnold J

更新日期：1992-07-25 00:00:00

abstract：:Numerous potential ligand-binding sites are available today, along with hundreds of thousands of known binding sites observed in the PDB. Exhaustive similarity search for such vastly numerous binding site pairs is useful to predict protein functions and to enable rapid screening of target proteins for drug design. Exi...

journal_title：Nucleic acids research

authors： Ito J,Tabei Y,Shimizu K,Tsuda K,Tomii K

更新日期：2012-01-01 00:00:00

abstract：:Although the human alpha-globin and beta-globin genes are co-regulated in adult life, they achieve the same end by very different mechanisms. For example, a transfected beta-globin gene is expressed in an inducible manner in mouse erythroleukemia (MEL) cells while a transfected alpha-globin gene is constitutively expr...

journal_title：Nucleic acids research

authors： Ren S,Li J,Atweh GF

更新日期：1996-01-15 00:00:00

abstract：:The transcriptional activity of the p53 tumor suppressor protein is crucial for the regulation of cell growth, apoptosis and tumor progression. The first identified p53 relative, p73, was reported to be monoallelically expressed in normal tissues. In some tumors, loss of heterozygosity was associated with overexpressi...

journal_title：Nucleic acids research

authors： Vikhanskaya F,D'Incalci M,Broggini M

更新日期：2000-01-15 00:00:00

abstract：:The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort among model plant genome databases and plant researchers that aims to create, maintain and facilitate the use of a controlled vocabulary (ontology) for plants. The ontology allows users to ascribe attributes of plant structure ...

journal_title：Nucleic acids research

authors： Avraham S,Tung CW,Ilic K,Jaiswal P,Kellogg EA,McCouch S,Pujar A,Reiser L,Rhee SY,Sachs MM,Schaeffer M,Stein L,Stevens P,Vincent L,Zapata F,Ware D

更新日期：2008-01-01 00:00:00

abstract：:The 5'- and 3'-untranslated regions (5'- and 3'-UTRs) of eukaryotic mRNAs are known to play a crucial role in post-transcriptional regulation of gene expression modulating nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and stability. UTRdb is a specialized database of 5' and 3' unt...

journal_title：Nucleic acids research

authors： Pesole G,Liuni S,Grillo G,Licciulli F,Mignone F,Gissi C,Saccone C

更新日期：2002-01-01 00:00:00

abstract：:Three Z-DNA-binding proteins of Mr 31, 33 and 58 kD were isolated from mature bull testis. They were obtained in a native state suitable for binding studies. These are the first examples of Z-DNA-binding proteins from a mammalian tissue. Purification involved tissue extraction with 0.35 M NaCl, cation exchange chromat...

journal_title：Nucleic acids research

authors： Gut SH,Bischoff M,Hobi R,Kuenzle CC

更新日期：1987-12-10 00:00:00

abstract：:A small family of DNA sequences is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which consti...

journal_title：Nucleic acids research

authors： Allitto BA,Karrer KM

更新日期：1986-10-24 00:00:00

abstract：:Treatment of the ciliated protozoan Tetrahymena thermophila with 8-methoxypsoralen combined with long wavelength ultraviolet irradiation (UVA, lambda approximately 360 nm) resulted in a dose dependent equal inhibition of the synthesis of rRNA, 5.8S, 5S and tRNA. Similar results were obtained with 3-carbethoxy-8-methox...

journal_title：Nucleic acids research

authors： Nielsen PE

更新日期：1987-02-11 00:00:00