Abstract:
:As shown in the accompanying paper (5), the oligonucleotide composition of the E. coli genome is highly asymmetric for sequences up to 6 bp in length when ranked from highest to lowest abundance. We show here that this largely reflects codon usage because heavily used codons were found in the highly abundant oligomers whereas rarely used codons, with some exceptions, occurred in sequences in low abundance. Furthermore, linear regression analysis revealed a strong correlation between the frequencies of each trinucleotide and its usage as a codon. Dinucleotides are also not randomly distributed across each codon position and the dinucleotide composition of genes that are transcribed but not translated (rRNA and tRNA genes) was highly related to that seen in genes encoding polypeptides. However, 45 tetra-, 8 penta-, and 6 hexanucleotides were significantly over- or underabundant by Markov chain analysis and could not be accounted for by codon usage. Of these underrepresented sequences, many were palindromes, including the Dam methylation site.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Phillips GJ,Arnold J,Ivarie Rdoi
10.1093/nar/15.6.2627subject
Has Abstractpub_date
1987-03-25 00:00:00pages
2627-38issue
6eissn
0305-1048issn
1362-4962journal_volume
15pub_type
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