Complete nucleotide sequence of the Escherichia coli recB gene.

abstract：:The availability of a draft human genome sequence and ability to monitor the transcription of thousands of genes with DNA microarrays has necessitated the need for new computational tools that can analyze cis-regulatory elements controlling genes that display similar expression patterns. We have developed a tool desig...

journal_title：Nucleic acids research

authors： Zhang H,Ramanathan Y,Soteropoulos P,Recce ML,Tolias PP

更新日期：2002-11-01 00:00:00

abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...

journal_title：Nucleic acids research

authors： Saintomé C,Amrane S,Mergny JL,Alberti P

更新日期：2016-04-07 00:00:00

abstract：:The precursor to the Euglena light harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) is a polyprotein containing multiple copies of LHCPII covalently joined by a decapeptide linker. cDNA and genomic clones encoding the 5' and 3' end of a 6.6 kb LHCPII mRNA were sequenced. A 3.1 kb genomic region en...

journal_title：Nucleic acids research

authors： Muchhal US,Schwartzbach SD

更新日期：1994-12-25 00:00:00

abstract：:The macronuclear genome of Euplotes crassus contains two different genes, EFA1 and EFA2, encoding EF-1alpha proteins. They are derived from micronuclear precursors in the course of a sexual process termed conjugation. We have found that two apparent micronuclear precursors exist for EFA1. They differ in their potentia...

journal_title：Nucleic acids research

authors： Florian V,Klein A

更新日期：1996-08-15 00:00:00

abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Ahmed Z,Lali SM,Sohail A

更新日期：1987-08-25 00:00:00

abstract：:The 5'- and 3'-untranslated regions (5'- and 3'-UTRs) of eukaryotic mRNAs are known to play a crucial role in post-transcriptional regulation of gene expression modulating nucleo-cytoplasmic mRNA transport, translation efficiency, subcellular localization and stability. UTRdb is a specialized database of 5' and 3' unt...

journal_title：Nucleic acids research

authors： Pesole G,Liuni S,Grillo G,Licciulli F,Mignone F,Gissi C,Saccone C

更新日期：2002-01-01 00:00:00

abstract：:The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory l...

journal_title：Nucleic acids research

authors： Bogdanova E,Djordjevic M,Papapanagiotou I,Heyduk T,Kneale G,Severinov K

更新日期：2008-03-01 00:00:00

abstract：:We present a simple and highly accurate computational method for operon prediction, based on intergenic distances and functional relationships between the protein products of contiguous genes, as defined by STRING database (Jensen,L.J., Kuhn,M., Stark,M., Chaffron,S., Creevey,C., Muller,J., Doerks,T., Julien,P., Roth,...

journal_title：Nucleic acids research

authors： Taboada B,Verde C,Merino E

更新日期：2010-07-01 00:00:00

abstract：:The mouse c-Ki-ras protooncogene promoter contains an unusual DNA element consisting of a 27 bp-long homopurine-homopyrimidine mirror repeat (H-motif) adjacent to a d(C-G)5 repeat. We have previously shown that in vitro these repeats may adopt H and Z conformations, respectively, causing nuclease and chemical hypersen...

journal_title：Nucleic acids research

authors： Raghu G,Tevosian S,Anant S,Subramanian KN,George DL,Mirkin SM

更新日期：1994-08-25 00:00:00

abstract：:We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micell...

journal_title：Nucleic acids research

authors： Thurmond KB 2nd,Remsen EE,Kowalewski T,Wooley KL

更新日期：1999-07-15 00:00:00

abstract：:TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro chromatin reconstitution system in combination with atomic force and fluorescence microscopic analyses to determine how ...

journal_title：Nucleic acids research

authors： Hizume K,Araki S,Yoshikawa K,Takeyasu K

更新日期：2007-01-01 00:00:00

abstract：:For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...

journal_title：Nucleic acids research

authors： Behn M,Schuermann M

更新日期：1998-03-01 00:00:00

abstract：:Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present. To understand translational regulation in this system, we systematically mutated AUGs 1-4 and monitored polypeptid...

journal_title：Nucleic acids research

authors： Wise HM,Barbezange C,Jagger BW,Dalton RM,Gog JR,Curran MD,Taubenberger JK,Anderson EC,Digard P

更新日期：2011-09-01 00:00:00

abstract：:Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein...

journal_title：Nucleic acids research

authors： Bryant KF,Cox JC,Wang H,Hogle JM,Ellington AD,Coen DM

更新日期：2005-10-24 00:00:00

abstract：:A problem in EST clustering is the presence of repeat sequences. To avoid false matches, repeats have to be masked. This can be a time-consuming process, and it depends on available repeat libraries. We present a fast and effective method that aims to eliminate the problems repeats cause in the process of clustering. ...

journal_title：Nucleic acids research

authors： Schneeberger K,Malde K,Coward E,Jonassen I

更新日期：2005-04-14 00:00:00

abstract：:The rat beta-casein gene is a member of a small gene family, encoding the principal milk proteins. In order to understand the mechanisms by which its stage- and tissue-specific expression are regulated, initially, a 14 kb genomic clone containing the entire 7.5 kb rat beta-casein gene with 3.5 kb of 5' and 3.0 kb of 3...

journal_title：Nucleic acids research

authors： Lee KF,DeMayo FJ,Atiee SH,Rosen JM

更新日期：1988-02-11 00:00:00

abstract：:The Eco57I restriction endonuclease and methylase were purified to homogeneity from the E.coli RR1 strain carrying the eco57IRM genes on a recombinant plasmid. The molecular weight of the denaturated methylase is 63 kDa. The restriction endonuclease exists in a monomeric form with an apparent molecular weight of 104-1...

journal_title：Nucleic acids research

authors： Janulaitis A,Petrusyte M,Maneliene Z,Klimasauskas S,Butkus V

更新日期：1992-11-25 00:00:00

abstract：:We have sequenced the Escherichia coli grpE gene and shown that it encodes a 197-amino acid residue protein of 21,668-Mr. The predicted N-terminal amino acid sequence, as well as the overall amino acid composition agree well with that of the purified protein. From Northern analysis, we have shown that transcription of...

journal_title：Nucleic acids research

authors： Lipinska B,King J,Ang D,Georgopoulos C

更新日期：1988-08-11 00:00:00

abstract：:Posttranslational modifications (PTMs) of histones represent a crucial regulatory mechanism of nucleosome and chromatin dynamics in various of DNA-based cellular processes, such as replication, transcription and DNA damage repair. Lysine succinylation (Ksucc) is a newly identified histone PTM, but its regulation and f...

journal_title：Nucleic acids research

authors： Jing Y,Ding D,Tian G,Kwan KCJ,Liu Z,Ishibashi T,Li XD

更新日期：2020-09-25 00:00:00

abstract：:Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studie...

journal_title：Nucleic acids research

authors： Newman JA,Savitsky P,Allerston CK,Bizard AH,Özer Ö,Sarlós K,Liu Y,Pardon E,Steyaert J,Hickson ID,Gileadi O

更新日期：2015-05-26 00:00:00

abstract：:Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a bio...

journal_title：Nucleic acids research

authors： Vazquez-Anderson J,Mihailovic MK,Baldridge KC,Reyes KG,Haning K,Cho SH,Amador P,Powell WB,Contreras LM

更新日期：2017-05-19 00:00:00

abstract：:TP63, a member of the p53 gene family gene, encodes the ΔNp63 protein and is one of the most frequently amplified genes in squamous cell carcinomas (SCC) of the head and neck (HNSCC) and lungs (LUSC). Using an epiallelic series of siRNAs with intrinsically different knockdown abilities, we show that the complete loss ...

journal_title：Nucleic acids research

authors： Bretz AC,Gittler MP,Charles JP,Gremke N,Eckhardt I,Mernberger M,Mandic R,Thomale J,Nist A,Wanzel M,Stiewe T

更新日期：2016-04-20 00:00:00

abstract：:Phage_Finder, a heuristic computer program, was created to identify prophage regions in completed bacterial genomes. Using a test dataset of 42 bacterial genomes whose prophages have been manually identified, Phage_Finder found 91% of the regions, resulting in 7% false positive and 9% false negative prophages. A searc...

journal_title：Nucleic acids research

authors： Fouts DE

更新日期：2006-01-01 00:00:00

abstract：:Genome-wide mapping in the identification of novel candidate genes has always been the standard method in genetics and genomics to correlate a clinically interesting phenotypic trait with a genotype. However, the performance of a mapping experiment using classical microsatellite approaches can be very time consuming. ...

journal_title：Nucleic acids research

authors： Klaften M,Hrabé de Angelis M

更新日期：2005-07-01 00:00:00

abstract：:Embryonic stem cells (ESCs) and meiosis are featured by relatively higher frequent homologous recombination associated with DNA double strand breaks (DSB) repair. Here, we show that Pold3 plays important roles in DSB repair, telomere maintenance and genomic stability of both ESCs and spermatocytes in mice. By attempti...

journal_title：Nucleic acids research

authors： Zhou Z,Wang L,Ge F,Gong P,Wang H,Wang F,Chen L,Liu L

更新日期：2018-04-20 00:00:00

abstract：:The nucleotide sequence of the celD gene, encoding the previously crystallized endoglucanase D of Clostridium thermocellum, is reported. The enzyme shares a conserved, reiterated domain with the COOH-terminal end of endoglucanases A and B from the same organism. The overexpression in Escherichia coli of celD subcloned...

journal_title：Nucleic acids research

authors： Joliff G,Béguin P,Aubert JP

更新日期：1986-11-11 00:00:00

abstract：:A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in the following order: (5') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3') (the subscripts giv...

journal_title：Nucleic acids research

authors： Bavykin SG,Usachenko SI,Lishanskaya AI,Shick VV,Belyavsky AV,Undritsov IM,Strokov AA,Zalenskaya IA,Mirzabekov AD

更新日期：1985-05-24 00:00:00

abstract：:The active 3D conformation of the spliceosome's catalytic U2/U6 RNA core is stabilised by a network of secondary and tertiary RNA interactions, but also depends on spliceosomal proteins for its formation. To determine the contribution towards splicing of specific RNA secondary and tertiary interactions in the U2/U6 RN...

journal_title：Nucleic acids research

authors： Bao P,Boon KL,Will CL,Hartmuth K,Lührmann R

更新日期：2018-12-14 00:00:00

abstract：:PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes s...

journal_title：Nucleic acids research

authors： Dutreix M,Bäckman A,Célérier J,Bagdasarian MM,Sommer S,Bailone A,Devoret R,Bagdasarian M

更新日期：1988-11-25 00:00:00

abstract：:RegPredict web server is designed to provide comparative genomics tools for reconstruction and analysis of microbial regulons using comparative genomics approach. The server allows the user to rapidly generate reference sets of regulons and regulatory motif profiles in a group of prokaryotic genomes. The new concept o...

journal_title：Nucleic acids research

authors： Novichkov PS,Rodionov DA,Stavrovskaya ED,Novichkova ES,Kazakov AE,Gelfand MS,Arkin AP,Mironov AA,Dubchak I

更新日期：2010-07-01 00:00:00