Abstract：
:Here, with the aim of obtaining insight into the intriguing selectivity of G-quadruplex (G4) ligands toward cancer compared to normal cells, a genetically controlled system of progressive transformation in human BJ fibroblasts was analyzed. Among the different comparative evaluations, we found a progressive increase of DNA damage response (DDR) markers throughout the genome from normal toward immortalized and transformed cells. More interestingly, sensitivity to G4 ligands strongly correlated with the presence of a basal level of DNA damage, including at the telomeres, where the chromosome ends were exposed to the DDR without concurrent induction of DNA repair activity, as revealed by the lack of 53BP1 recruitment and telomere aberrations. The link between telomere uncapping and the response to G4 stabilization was directly assessed by showing that a partial TRF2 depletion, causing a basal level of telomere localized DDR, rendered telomerized fibroblasts prone to G4-induced telomere damage and anti-proliferative defects. Taken together these data strongly indicate that the presence of a basal level of telomere-associated DDR is a determinant of susceptibility to G4 stabilization.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Salvati E,Rizzo A,Iachettini S,Zizza P,Cingolani C,D'Angelo C,Porru M,Mondello C,Aiello A,Farsetti A,Gilson E,Leonetti C,Biroccio Adoi
10.1093/nar/gkv006subject
Has Abstractpub_date
2015-02-18 00:00:00pages
1759-69issue
3eissn
0305-1048issn
1362-4962pii
gkv006journal_volume
43pub_type
杂志文章abstract：:The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2-10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa086
更新日期：2020-04-06 00:00:00
abstract：:The ribosomal proteins L4 and L22 form part of the peptide exit tunnel in the large ribosomal subunit. In Escherichia coli, alterations in either of these proteins can confer resistance to the macrolide antibiotic, erythromycin. The structures of the 30S as well as the 50S subunits from each antibiotic resistant mutan...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh913
更新日期：2004-10-27 00:00:00
abstract：:The accumulation of somatic mitochondrial DNA (mtDNA) mutations contributes to the pathogenesis of human disease. Currently, mitochondrial mutations are largely considered results of inaccurate processing of its heavily damaged genome. However, mainly from a lack of methods to monitor mtDNA mutations with sufficient s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw716
更新日期：2016-10-14 00:00:00
abstract：:Extensive research has characterized distinct exogenous RNAi pathways interfering in gene expression during vegetative growth of the unicellular model ciliate Paramecium. However, role of RNAi in endogenous transcriptome regulation, and environmental adaptation is unknown. Here, we describe the first genome-wide profi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz553
更新日期：2019-09-05 00:00:00
abstract：:Ribosomal RNA (rRNA) in intact ribosomes is cleaved into discrete products on incubation of reticulocyte lysates or L-cell extracts with ppp(A2'p)3A. Cleavage of rRNA may, therefore, provide a useful assay for 2-5A (ppp)A2'p)nA; n = 2 to 4) or for the presence of a 2-5A-dependent nuclease. The results with reticulocyt...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.7.1571
更新日期：1981-04-10 00:00:00
abstract：:The macronuclear genome of Euplotes crassus contains two different genes, EFA1 and EFA2, encoding EF-1alpha proteins. They are derived from micronuclear precursors in the course of a sexual process termed conjugation. We have found that two apparent micronuclear precursors exist for EFA1. They differ in their potentia...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.16.3195
更新日期：1996-08-15 00:00:00
abstract：:A prototype of a novel class of DNA alkylating agents, which combines the DNA crosslinking moiety chlorambucil (Chl) with a sequence-selective hairpin pyrrole-imidazole polyamide ImPy-beta-ImPy-gamma-ImPy-beta-Dp (polyamide 1), was evaluated for its ability to damage DNA and induce biological responses. Polyamide 1-Ch...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg215
更新日期：2003-02-15 00:00:00
abstract：:Pathbase is a database that stores images of the abnormal histology associated with spontaneous and induced mutations of both embryonic and adult mice including those produced by transgenesis, targeted mutagenesis and chemical mutagenesis. Images of normal mouse histology and strain-dependent background lesions are al...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh124
更新日期：2004-01-01 00:00:00
abstract：:The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.12.3043
更新日期：1992-06-25 00:00:00
abstract：:We have established a sensitive, monoclonal antibody (Mab)-based procedure permitting the selective enrichment of sequences containing the miscoding alkylation product O6-ethylguanine (O6-EtGua) from mammalian DNA. H5 rat hepatoma cells were reacted with the N-nitroso carcinogen N-ethyl-N-nitrosourea in vitro, to give...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.16.4467
更新日期：1991-08-25 00:00:00
abstract：:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.3.470
更新日期：1996-02-01 00:00:00
abstract：:Array based DNA pooling techniques facilitate genome-wide scale genotyping of large samples. We describe a structured analysis method for pooled data using internal replication information in large scale genotyping sets. The method takes advantage of information from single nucleotide polymorphisms (SNPs) typed in par...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl136
更新日期：2006-04-20 00:00:00
abstract：:G-quadruplexes play various roles in multiple biological processes, which can be positive when a G4 is involved in the regulation of gene expression or detrimental when the folding of a stable G4 impairs DNA replication promoting genome instability. This duality interrogates the significance of their presence within g...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz463
更新日期：2019-07-09 00:00:00
abstract：:Details of polynucleotide flexibility may be probed through a combination of semiempirical potential energy calculations and statistical mechanical analyses. The pseudorotational motions of the furanose and the long-range correlated rotations of the chain backbone are described briefly here. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.3.777
更新日期：1982-02-11 00:00:00
abstract：:SplicePort is a web-based tool for splice-site analysis that allows the user to make splice-site predictions for submitted sequences. In addition, the user can also browse the rich catalog of features that underlies these predictions, and which we have found capable of providing high classification accuracy on human s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm407
更新日期：2007-07-01 00:00:00
abstract：:Three Z-DNA-binding proteins of Mr 31, 33 and 58 kD were isolated from mature bull testis. They were obtained in a native state suitable for binding studies. These are the first examples of Z-DNA-binding proteins from a mammalian tissue. Purification involved tissue extraction with 0.35 M NaCl, cation exchange chromat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.23.9691
更新日期：1987-12-10 00:00:00
abstract：:Reversion of T type cytoplasmic male sterility (cmsT) to fertility is correlated with sequence changes in a 1.5 kb AvaI fragment. This 1.5 kb AvaI fragment is composed of 5' flanking sequences of ATPase subunit 6, one complete open reading frame (ORF 13) and part of the another (ORF 25). The sequence of the 1.5 kb Ava...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.15.6091
更新日期：1987-08-11 00:00:00
abstract：:The transcriptional activity of a human transposon-like family of repeats, called the THE-1 family, has been studied in cell culture and in human tissue. Both strands of THE-1 are present in several discrete length poly A plus RNAs. Primer extension studies and the structures of cDNA clones show that these THE-1 trans...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.13.5199
更新日期：1987-07-10 00:00:00
abstract：:Telomeric regions of the genome are inherently difficult-to-replicate due to their propensity to generate DNA secondary structures and form nucleoprotein complexes that can impede DNA replication fork progression. Precisely how cells respond to DNA replication stalling within a telomere remains poorly characterized, l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz158
更新日期：2019-05-21 00:00:00
abstract：:Conserved in a variety of evolutionarily divergent plant species, LOB DOMAIN (LBD) genes define a large, plant-specific family of largely unknown function. LBD genes have been implicated in a variety of developmental processes in plants, although to date, relatively few members have been assigned functions. LBD protei...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm775
更新日期：2007-01-01 00:00:00
abstract：:The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to syn...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.22.6595
更新日期：1990-11-25 00:00:00
abstract：:The distribution and amount of 5-methylcytosine (5-MeCyt) in DNA was measured for early embryos of mouse strain CF1 (2 to 4 cell stage to blastocyst) and mouse teratocarcinoma cells. In each case, the pattern of methylation was examined by use of the restriction enzymes Hha I and HPA II HPA II, which cut DNA at the si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.8.2369
更新日期：1979-12-20 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1999-11-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：2017-01-04 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1994-08-11 00:00:00
abstract：:Active DNA demethylation is crucial for epigenetic control, but the underlying enzymatic mechanisms are incompletely understood. REPRESSOR OF SILENCING 1 (ROS1) is a 5-methylcytosine (5-meC) DNA glycosylase/lyase that initiates DNA demethylation in plants through a base excision repair process. The enzyme binds DNA no...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt625
更新日期：2013-10-01 00:00:00
abstract：:The Protein Folding Database (PFD) is a publicly accessible repository of thermodynamic and kinetic protein folding data. Here we describe the first major revision of this work, featuring extensive restructuring that conforms to standards set out by the recently formed International Foldeomics Consortium. The database...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1007
更新日期：2007-01-01 00:00:00
abstract：:The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.12.3337
更新日期：1976-12-01 00:00:00
abstract：:We have examined the transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 in UV irradiated cells, in the mitotic cell cycle, and during meiosis. Levels of RAD18 mRNA increased upon UV irradiation, but remained constant during the mitotic cell cycle. During meiosis, RAD18 mRNA levels rose about 4 fol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.4.893
更新日期：1991-02-25 00:00:00
