Pathbase: a database of mutant mouse pathology.

abstract：:DNA-binding and RNA-binding proteins are usually considered 'undruggable' partly due to the lack of an efficient method to identify inhibitors from existing small molecule repositories. Here we report a rapid and sensitive high-throughput screening approach to identify compounds targeting protein-nucleic acids interac...

journal_title：Nucleic acids research

authors： Alonso N,Guillen R,Chambers JW,Leng F

更新日期：2015-04-30 00:00:00

abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...

journal_title：Nucleic acids research

authors： Cupane A,Vitrano E,San Biago PL,Madonia F,Palma MU

更新日期：1980-09-25 00:00:00

abstract：:The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for ...

journal_title：Nucleic acids research

authors： Guilley H,Jonard G,Kukla B,Richards KE

更新日期：1979-04-01 00:00:00

abstract：:Linkage analysis is a successful procedure to associate diseases with specific genomic regions. These regions are often large, containing hundreds of genes, which make experimental methods employed to identify the disease gene arduous and expensive. We present two methods to prioritize candidates for further experimen...

journal_title：Nucleic acids research

authors： George RA,Liu JY,Feng LL,Bryson-Richardson RJ,Fatkin D,Wouters MA

更新日期：2006-01-01 00:00:00

abstract：:Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5'-TTTN-3' protospacer adjacent motif (PAM) at the 5' end of the protospacer for genome editing. For widespread application in...

journal_title：Nucleic acids research

authors： Tu M,Lin L,Cheng Y,He X,Sun H,Xie H,Fu J,Liu C,Li J,Chen D,Xi H,Xue D,Liu Q,Zhao J,Gao C,Song Z,Qu J,Gu F

更新日期：2017-11-02 00:00:00

abstract：:Laser-Raman spectra of poly(rI) show the formation of an ordered complex in aqueous solutions of high ionic strength. This structure exhibits the A-helix geometry, contains stacked bases and is apparently stabilized by specific hydrogen bonding involving hypoxanthine C6=0 groups. Thermal dissociation of the poly(rI) c...

journal_title：Nucleic acids research

authors： Chou CH,Thomas GJ Jr,Arnott S,Smith PJ

更新日期：1977-07-01 00:00:00

abstract：:The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital importance for transcription, DNA replication and genome maintenance. Each of these activities is tightly regulated in both time and space. While we have a good understanding of chromatin organization in space, for example in fixe...

journal_title：Nucleic acids research

authors： Shaban HA,Seeber A

更新日期：2020-04-17 00:00:00

abstract：:The task of gene identification frequently confronting researchers working with both novel and well studied genomes can be conveniently and reliably solved with the help of the GeneMark web software (http://opal.biology.gatech.edu/GeneMark/). The website provides interfaces to the GeneMark family of programs designed ...

journal_title：Nucleic acids research

authors： Besemer J,Borodovsky M

更新日期：2005-07-01 00:00:00

abstract：:The pol gene of all retroviruses is expressed as a gag-pol fusion protein which is proteolytically processed to produce all viral enzymes. In the human immunodeficiency virus (HIV), the gag and pol genes overlap by 241 nucleotides with pol in the -1 phase with respect to gag. The gag-pol fusion is produced via a -1 ri...

journal_title：Nucleic acids research

authors： Vickers TA,Ecker DJ

更新日期：1992-08-11 00:00:00

abstract：:Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show t...

journal_title：Nucleic acids research

authors： Bosio MC,Fermi B,Spagnoli G,Levati E,Rubbi L,Ferrari R,Pellegrini M,Dieci G

更新日期：2017-05-05 00:00:00

abstract：:Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system. The analysis of labeled reaction products on specific gel systems suggests that the...

journal_title：Nucleic acids research

authors： Backendorf C,Olsthoorn R,van de Putte P

更新日期：1989-12-25 00:00:00

abstract：:Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...

journal_title：Nucleic acids research

authors： Pech A,Achenbach J,Jahnz M,Schülzchen S,Jarosch F,Bordusa F,Klussmann S

更新日期：2017-04-20 00:00:00

abstract：:The Human Genome Project has facilitated the sequencing of many species, yet the current Sanger method is too expensive, labor intensive and time consuming to accomplish medical resequencing of human genomes en masse. Of the 'next-generation' technologies, cyclic reversible termination (CRT) is a promising method with...

journal_title：Nucleic acids research

authors： Wu W,Stupi BP,Litosh VA,Mansouri D,Farley D,Morris S,Metzker S,Metzker ML

更新日期：2007-01-01 00:00:00

abstract：:Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected mola...

journal_title：Nucleic acids research

authors： Gegenheimer P

更新日期：1988-03-11 00:00:00

abstract：:Programmed chromosome breakage occurs at 50-200 specific sites in the genome of Tetrahymena thermo-phila during somatic nuclear (macronuclear) differentiation. Previous studies have identified a 15 bp sequence, the Cbs (for chromosome breakage sequence), that is necessary and sufficient to specify these sites. In this...

journal_title：Nucleic acids research

authors： Fan Q,Yao MC

更新日期：2000-02-15 00:00:00

abstract：:The SPARC gene 5'flanking sequence has been shown to contain enhancer elements, but also negative control elements immediately upstream of the enhancer elements. Although these 5'enhancer elements are active in F9 and PYS-2 cells, their activities are nullified by the 5'repressor activity. In the present study we have...

journal_title：Nucleic acids research

authors： Satyamoorthy K,Samulewicz SJ,Thornburg LD,Basu A,Howe CC

更新日期：1997-08-01 00:00:00

abstract：:The FALC-Loop web server provides an online interface for protein loop modeling by employing an ab initio loop modeling method called FALC (fragment assembly and analytical loop closure). The server may be used to construct loop regions in homology modeling, to refine unreliable loop regions in experimental structures...

journal_title：Nucleic acids research

authors： Ko J,Lee D,Park H,Coutsias EA,Lee J,Seok C

更新日期：2011-07-01 00:00:00

abstract：:Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B respectively. Analysis of in vitro replication products by two-dimensional agar...

journal_title：Nucleic acids research

authors： Kunnimalaiyaan M,Nielsen BL

更新日期：1997-09-15 00:00:00

abstract：:The genes for mitoribosomal protein S12 (Mrps12) and mitochondrial seryl-tRNA ligase (Sarsm and Sars2) are oppositely transcribed from a conserved promoter region of <200 bp in both human and mouse. Using a dual reporter vector we identified an array of 4 CCAAT box elements required for efficient transcription of the ...

journal_title：Nucleic acids research

authors： Zanotto E,Shah ZH,Jacobs HT

更新日期：2007-01-01 00:00:00

abstract：:Little is known about the function of most non-coding RNAs (ncRNAs). The majority of long ncRNAs (lncRNAs) is expressed at very low levels and it is a matter of intense debate whether these can be of functional relevance. Here, we identified lncRNAs regulating the viability of lung cancer cells in a high-throughput RN...

journal_title：Nucleic acids research

authors： Seiler J,Breinig M,Caudron-Herger M,Polycarpou-Schwarz M,Boutros M,Diederichs S

更新日期：2017-05-19 00:00:00

abstract：:A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data, especially those obtained by the sequencing method using M13 phages. Programs NUCDAT and PROTEN are for analyzing DNA/RNA and...

journal_title：Nucleic acids research

authors： Isono K

更新日期：1984-01-11 00:00:00

abstract：:Heterotrimeric eukaryotic/archaeal translation initiation factor 2 (e/aIF2) binds initiator methionyl-tRNA and plays a key role in the selection of the start codon on messenger RNA. tRNA binding was extensively studied in the archaeal system. The γ subunit is able to bind tRNA, but the α subunit is required to reach h...

journal_title：Nucleic acids research

authors： Naveau M,Lazennec-Schurdevin C,Panvert M,Dubiez E,Mechulam Y,Schmitt E

更新日期：2013-01-01 00:00:00

abstract：:Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma. The plasmid, named L factor, was found at a high copy number (5,000 or more per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA co...

journal_title：Nucleic acids research

authors： Saito H,Uehara H,Kusano T,Oishi M

更新日期：1987-11-11 00:00:00

abstract：:The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which...

journal_title：Nucleic acids research

authors： Fleck O,Michael H,Heim L

更新日期：1992-05-11 00:00:00

abstract：:On the tandemly linked ribosomal genes of Xenopus laevis, the RNA polymerase transcribes past the 3' end of the 40S coding region and terminates at T3 just upstream of the gene promoter. The close proximity of T3 to the gene promoter, and the functional interdependence of these two elements, has led to the suggestion ...

journal_title：Nucleic acids research

authors： Lucchini R,Reeder RH

更新日期：1989-01-11 00:00:00

abstract：:Human foamy or spuma virus (HFV) codes for a distinct set of pol gen products. To determine the minimal requirements for the HFV enzymatic activities, defined residues of the reverse transcriptase (RT) and ribo-nuclease H (RNase H) domain of the HFV pol gene were mutated by site-specific PCR mutagenesis. The mutant ge...

journal_title：Nucleic acids research

authors： Kögel D,Aboud M,Flügel RM

更新日期：1995-07-25 00:00:00

abstract：:VA RNA(I) is a non-coding adenoviral transcript that counteracts the host cell anti-viral defenses such as immune responses mediated via PKR. We investigated potential alternate secondary structure conformations within the PKR-binding domain of VA RNA(I) using site-directed mutagenesis, RNA UV-melting analysis and enz...

journal_title：Nucleic acids research

authors： Wahid AM,Coventry VK,Conn GL

更新日期：2009-09-01 00:00:00

abstract：:The higher order structure of the functionally important 530 loop in Escherichia coli 16S rRNA was studied in mutants with single base changes at position 517, which significantly impair translational fidelity. The 530 loop has been proposed to interact with the EF-Tu-GTP-aatRNA ternary complex during decoding. The re...

journal_title：Nucleic acids research

authors： Van Ryk DI,Dahlberg AE

更新日期：1995-09-11 00:00:00

abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...

journal_title：Nucleic acids research

authors： Burel SA,Hart CE,Cauntay P,Hsiao J,Machemer T,Katz M,Watt A,Bui HH,Younis H,Sabripour M,Freier SM,Hung G,Dan A,Prakash TP,Seth PP,Swayze EE,Bennett CF,Crooke ST,Henry SP

更新日期：2016-03-18 00:00:00

abstract：:Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...

journal_title：Nucleic acids research

authors： Tsai-Morris CH,Cao XM,Sukhatme VP

更新日期：1988-09-26 00:00:00