Abstract:
:Purified UvrA, UvrB, UvrC, UvrD, PolA and Lig proteins from Escherichia coli have been used to assess the effect of nucleotide excision repair on the conformation of native negatively supercoiled plasmid DNA in an in vitro test system. The analysis of labeled reaction products on specific gel systems suggests that the Uvr excinuclease has the ability to restrain the superhelical stress in the template DNA during the repair process. This feature, observed in the case of the Uvr system is not found if the repair reaction is initiated by T4 endonuclease V or Micrococcus luteus UV endonuclease.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Backendorf C,Olsthoorn R,van de Putte Pdoi
10.1093/nar/17.24.10337subject
Has Abstractpub_date
1989-12-25 00:00:00pages
10337-51issue
24eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract::A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki762
更新日期:2005-08-09 00:00:00
abstract::The ProtoNet site provides an automatic hierarchical clustering of the SWISS-PROT protein database. The clustering is based on an all-against-all BLAST similarity search. The similarities' E-score is used to perform a continuous bottom-up clustering process by applying alternative rules for merging clusters. The outco...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg096
更新日期:2003-01-01 00:00:00
abstract::Stability of aromatic phosphoramidates was studied using 2',3'-O-dibenzoyluridine 5'-phosphoramidates and N,2',3'-O-tribenzoylcytidine 5'-phosphate. The effect of dicyclohexylcarbodiimide in this mixture was investigated. Decomposition of the anilidate was slower in the presence of DCC. Substituted anilidates of uridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.10.2331
更新日期:1993-05-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1085
更新日期:2019-01-08 00:00:00
abstract::Loss-of-function (LOF) methods such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing provide unparalleled power for studying the biological function of genes of interest. However, a major concern is non-specific targeting, which involves depletion of transcripts other than those in...
journal_title:Nucleic acids research
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq565
更新日期:2010-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:2004-07-01 00:00:00
abstract::Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-09-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki913
更新日期:2005-10-27 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1984-01-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz944
更新日期:2020-01-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.17.5133
更新日期:1990-09-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:2020-01-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1984-02-10 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.8.3385
更新日期:1987-04-24 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2015-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...
journal_title:Nucleic acids research
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