Abstract：
:The chromatin structure of a germ-line specific gene, the TPG-3 gene, one of the rainbow trout protamine genes was analyzed in various tissues. The protamine genes are expressed in early stage testis but not in late stage testis, liver or erythrocyte. Five potential CpG methylation sites in the coding and flanking regions of the TPG-3 protamine gene were monitored in early and late stage testis, nucleoprotamine, liver and erythrocyte. In all cases the patterns of methylation were identical with only one CpG site at position -740 being methylated. Thus, the methylation pattern of this protamine gene remained the same independently of the expression of the gene. Two Msp I sites at positions -293 and/or -275 and +155 were accessible to the enzyme in the TPG-3 chromatin of early stage testis. Since the Msp I site at position -293 and/or -275 was also present in the TPG-3 chromatin of liver, only the site at position +155 within the transcribed region correlated with the expression of the protamine gene.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Delcuve GP,Davie JRdoi
10.1093/nar/15.8.3385subject
Has Abstractpub_date
1987-04-24 00:00:00pages
3385-96issue
8eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract：:The Agrobacterium tumefaciens Ti plasmid contains at least six transcriptional units (designated vir loci) which are essential for efficient crown gall tumorigenesis. Mutations in one of these loci, virE, result in a sharply attenuated virulence phenotype. In the present communication, we have analyzed the virE operon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.2.825
更新日期：1987-01-26 00:00:00
abstract：:The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1337
更新日期：2016-01-04 00:00:00
abstract：:The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.19.3790
更新日期：1996-10-01 00:00:00
abstract：:An oligonucleotide hybrid is described which possesses two triple helix forming oligonucleotides which have been connected by a flexible polymeric linker chain. As a prototype, binding of this class of oligonucleotide to duplex DNA has been studied using a segment of the HSV-1 D-glycoprotein promoter, which possesses ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.20.4810
更新日期：1993-10-11 00:00:00
abstract：:Eukaryotic genomes contain many endogenous retroviral sequences (ERVs). ERVs are often severely mutated, therefore difficult to detect. A platform independent (Java) program package, RetroTector (ReTe), was constructed. It has three basic modules: (i) detection of candidate long terminal repeats (LTRs), (ii) detection...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm515
更新日期：2007-01-01 00:00:00
abstract：:We describe PReMod, a new database of genome-wide cis-regulatory module (CRM) predictions for both the human and the mouse genomes. The prediction algorithm, described previously in Blanchette et al. (2006) Genome Res., 16, 656-668, exploits the fact that many known CRMs are made of clusters of phylogenetically conser...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl879
更新日期：2007-01-01 00:00:00
abstract：:Numerous examples exist of how disrupting the actions of physiological regulators of blood cell development yields hematologic malignancies. The master regulator of hematopoietic stem/progenitor cells GATA-2 was cloned almost 20 years ago, and elegant genetic analyses demonstrated its essential function to promote hem...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
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更新日期：2012-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.21.6353
更新日期：1990-11-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt795
更新日期：2013-12-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm587
更新日期：2007-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.3.590
更新日期：1997-02-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq661
更新日期：2010-12-01 00:00:00
abstract：:The Comparative Toxicogenomics Database (CTD; http://ctdbase.org/) provides information about interactions between environmental chemicals and gene products and their relationships to diseases. Chemical-gene, chemical-disease and gene-disease interactions manually curated from the literature are integrated to generate...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks994
更新日期：2013-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp210
更新日期：2009-06-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh825
更新日期：2004-09-23 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1761
更新日期：1998-04-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/27.5.1243
更新日期：1999-03-01 00:00:00
abstract：:Human transcription factor IIIC contains a 55 kDa polypeptide which specifically interacts with the Adenovirus 2 VAI gene promoter and which mimics most of the DNA binding properties of the entire factor. The specificity and affinity of this protein:DNA interaction was demonstrated by: (i) Separation of purified fract...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.16.4743
更新日期：1990-08-25 00:00:00
abstract：:Human Ape2 protein has 3' phosphodiesterase activity for processing 3'-damaged DNA termini, 3'-5' exonuclease activity that supports removal of mismatched nucleotides from the 3'-end of DNA, and a somewhat weak AP-endonuclease activity. However, very little is known about the role of Ape2 in DNA repair processes. Here...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp357
更新日期：2009-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1088
更新日期：2012-01-01 00:00:00
abstract：:DNA polymerases of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl group on the incoming nucleotide. In the family Y (lesion bypass) polyme...
journal_title：Nucleic acids research
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doi：10.1093/nar/gkg417
更新日期：2003-07-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz725
更新日期：2019-10-10 00:00:00
abstract：:The role of Ribonuclease H1 (RNase H1) during primer removal and ligation at the mitochondrial origin of light-strand DNA synthesis (OriL) is a key, yet poorly understood, step in mitochondrial DNA maintenance. Here, we reconstitute the replication cycle of L-strand synthesis in vitro using recombinant mitochondrial p...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky708
更新日期：2018-10-12 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw945
更新日期：2017-01-04 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv276
更新日期：2015-04-30 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.12.2223
更新日期：1975-12-01 00:00:00
abstract：:The widely accepted model for the processing of tRNAs in Escherichia coli involves essential initial cleavages by RNase E within polycistronic transcripts to generate pre-tRNAs that subsequently become substrates for RNase P. However, recently we identified two polycistronic tRNA transcripts whose endonucleolytic proc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm991
更新日期：2008-02-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.17.3935
更新日期：1993-08-25 00:00:00
abstract：:We have developed an information repository named SpliceInfo to collect the occurrences of the four major alternative-splicing (AS) modes in human genome; these include exon skipping, 5'-alternative splicing, 3'-alternative splicing and intron retention. The dataset is derived by comparing the nucleotide and protein s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki129
更新日期：2005-01-01 00:00:00
abstract：:We developed an algorithm named ViReMa (Viral-Recombination-Mapper) to provide a versatile platform for rapid, sensitive and nucleotide-resolution detection of recombination junctions in viral genomes using next-generation sequencing data. Rather than mapping read segments of pre-defined lengths and positions, ViReMa ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt916
更新日期：2014-01-01 00:00:00
