Abstract:
:Recent progress in predicting RNA structure is moving towards filling the 'gap' in 2D RNA structure prediction where, for example, predicted internal loops often form non-canonical base pairs. This is increasingly recognized with the steady increase of known RNA 3D modules. There is a general interest in matching structural modules known from one molecule to other molecules for which the 3D structure is not known yet. We have created a pipeline, metaRNAmodules, which completely automates extracting putative modules from the FR3D database and mapping of such modules to Rfam alignments to obtain comparative evidence. Subsequently, the modules, initially represented by a graph, are turned into models for the RMDetect program, which allows to test their discriminative power using real and randomized Rfam alignments. An initial extraction of 22 495 3D modules in all PDB files results in 977 internal loop and 17 hairpin modules with clear discriminatory power. Many of these modules describe only minor variants of each other. Indeed, mapping of the modules onto Rfam families results in 35 unique locations in 11 different families. The metaRNAmodules pipeline source for the internal loop modules is available at http://rth.dk/resources/mrm.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Theis C,Höner Zu Siederdissen C,Hofacker IL,Gorodkin Jdoi
10.1093/nar/gkt795subject
Has Abstractpub_date
2013-12-01 00:00:00pages
9999-10009issue
22eissn
0305-1048issn
1362-4962pii
gkt795journal_volume
41pub_type
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