Abstract:
:The Agrobacterium tumefaciens Ti plasmid contains at least six transcriptional units (designated vir loci) which are essential for efficient crown gall tumorigenesis. Mutations in one of these loci, virE, result in a sharply attenuated virulence phenotype. In the present communication, we have analyzed the virE operon at the molecular level. This locus contains open reading frames coding for two hydrophilic proteins having molecular weights of approximately 7,000 daltons and 60,500 daltons. Using a maxicell strain of E. coli, we have visualized two proteins encoded by virE which correspond in size to these open reading frames. Analysis of codon usage of virE and seven other vir loci indicates that, in contrast to E. coli, all possible codons for a given amino acid are utilized at approximately the same frequency.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Winans SC,Allenza P,Stachel SE,McBride KE,Nester EWdoi
10.1093/nar/15.2.825subject
Has Abstractpub_date
1987-01-26 00:00:00pages
825-37issue
2eissn
0305-1048issn
1362-4962journal_volume
15pub_type
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