Abstract:
:In Eukaryotes, DNA is wound around the histone octamer forming the basic chromatin unit, the nucleosome. Atomic structures have been obtained from crystallography and single particle cryo-electron microscopy (cryoEM) of identical engineered particles. But native nucleosomes are dynamical entities with diverse DNA sequence and histone content, and little is known about their conformational variability, especially in the cellular context. Using cryoEM and tomography of vitreous sections we analyse native nucleosomes, both in vitro, using purified particles solubilized at physiologically relevant concentrations (25-50%), and in situ, within interphase nuclei. We visualize individual nucleosomes at a level of detail that allows us to measure the distance between the DNA gyres wrapped around. In concentrated solutions, we demonstrate a salt-dependent transition, with a high salt compact conformation resembling the canonical nucleosome and an open low salt one, closer to nuclear nucleosomes. Although further particle characterization and cartography are needed to understand the relationship between this conformational variability and chromatin functional states, this work opens a route to chromatin exploration in situ.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Eltsov M,Grewe D,Lemercier N,Frangakis A,Livolant F,Leforestier Adoi
10.1093/nar/gky670subject
Has Abstractpub_date
2018-09-28 00:00:00pages
9189-9200issue
17eissn
0305-1048issn
1362-4962pii
5058967journal_volume
46pub_type
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