Eukaryotic rpL10 drives ribosomal rotation.

abstract：:Release of RNA polymerase II (Pol II) from promoter-proximal pausing has emerged as a critical step regulating gene expression in multicellular organisms. The transition of Pol II into productive elongation requires the kinase activity of positive transcription elongation factor b (P-TEFb), which is itself under a str...

journal_title：Nucleic acids research

authors： C Quaresma AJ,Bugai A,Barboric M

更新日期：2016-09-19 00:00:00

abstract：:The hypoxia-inducible factor-1 (HIF-1) was first described as a DNA binding activity that specifically recognizes an 8 bp motif known to be essential for hypoxia-inducible erythropoietin gene transcription. Subsequently HIF-1 activity has also been found in cell lines which do not express erythropoietin, suggesting th...

journal_title：Nucleic acids research

authors： Kvietikova I,Wenger RH,Marti HH,Gassmann M

更新日期：1995-11-25 00:00:00

abstract：:The primary structures of two overlapping novel alpha-tubulin cDNA clones isolated from a Macaca fascicularis testis cDNA library and the corresponding human gene are presented. Although the general structure of the human gene conforms to that of previously described mammalian alpha-tubulin genes, there is a surprisin...

journal_title：Nucleic acids research

authors： Dobner PR,Kislauskis E,Wentworth BM,Villa-Komaroff L

更新日期：1987-01-12 00:00:00

abstract：:The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...

journal_title：Nucleic acids research

authors： Kumar G,Cuypers M,Webby RR,Webb TR,White SW

更新日期：2021-01-19 00:00:00

abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...

journal_title：Nucleic acids research

authors： Turro E,Lewin A,Rose A,Dallman MJ,Richardson S

更新日期：2010-01-01 00:00:00

abstract：:Members of the HMG-I(Y) family of mammalian nonhistone proteins are of importance because they have been demonstrated to bind specifically to the minor groove of A.T-rich sequences both in vitro and in vivo and to function as gene transcriptional regulatory proteins in vivo. Here we report the cloning, sequencing, cha...

journal_title：Nucleic acids research

authors： Friedmann M,Holth LT,Zoghbi HY,Reeves R

更新日期：1993-09-11 00:00:00

abstract：:The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...

journal_title：Nucleic acids research

authors： Gerhart E,Wagner H,Nordström K

更新日期：1986-03-25 00:00:00

abstract：:Hepatocyte Nuclear Factor 4 (HNF-4), a liver-enriched orphan receptor of the nuclear receptor superfamily, is required for the expression of a wide variety of liver-specific genes including apoAI. To explore the possibility that site A of the apoAI gene enhancer might also be the target for HNF-4 without the interfere...

journal_title：Nucleic acids research

authors： Fuernkranz HA,Wang Y,Karathanasis SK,Mak P

更新日期：1994-12-25 00:00:00

abstract：:We have overproduced and purified wild type regA protein, a translational repressor encoded by bacteriophage T4. The repressor activity of the cloned regA protein has been tested on four known regA target genes (T4 genes: 44, 45, rpbA and regA) using in vitro coupled transcription-translation reactions. We have demons...

journal_title：Nucleic acids research

authors： Webster KR,Adari HY,Spicer EK

更新日期：1989-12-11 00:00:00

abstract：:The human transcription factor EF-1A binds to the purine-rich E1A core enhancer sequence in the adenovirus E1A and E4 and polyomavirus enhancer regions. The consensus binding site for EF-1A resembles that of members of the ets domain protein family. EF-1A activation of transcription requires a dimeric binding site. An...

journal_title：Nucleic acids research

authors： Bolwig GM,Bruder JT,Hearing P

更新日期：1992-12-25 00:00:00

abstract：:A cross index is presented for using the improved selectivity offered by the Hung and Wensink (Nucl. Acids Res. 12, 1863-1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be jo...

journal_title：Nucleic acids research

authors： Korch C

更新日期：1987-04-24 00:00:00

abstract：:Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three ger...

journal_title：Nucleic acids research

authors： Park SJ,Komiyama Y,Suemori H,Umezawa A,Nakai K

更新日期：2016-01-04 00:00:00

abstract：:The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, ...

journal_title：Nucleic acids research

authors： Pestov DG,Dayn A,Siyanova EYu,George DL,Mirkin SM

更新日期：1991-12-11 00:00:00

abstract：:A novel resource centre for TP53 mutations and mutants has been developed (http://p53.fr). TP53 gene dysfunction can be found in the majority of human cancer types. The potential use of TP53 mutation as a biomarker for clinical studies or exposome analysis has led to the publication of thousands of reports describing ...

journal_title：Nucleic acids research

authors： Leroy B,Fournier JL,Ishioka C,Monti P,Inga A,Fronza G,Soussi T

更新日期：2013-01-01 00:00:00

abstract：:Cellular RNAs that do not function as messenger RNAs (mRNAs), transfer RNAs (tRNAs) or ribosomal RNAs (rRNAs) comprise a diverse class of molecules that are commonly referred to as non-protein-coding RNAs (ncRNAs). These molecules have been known for quite a while, but their importance was not fully appreciated until ...

journal_title：Nucleic acids research

authors： Hüttenhofer A,Vogel J

更新日期：2006-01-25 00:00:00

abstract：:Thyroglobulin (Tg), the precursor of thyroid hormones, is a 660.000 Da dimeric glycoprotein synthesized exclusively in the thyroid gland. We have cloned the human thyroglobulin gene from cosmid and phage libraries and constructed a complete restriction map. The gene encodes an 8.7 kb mRNA, covers at least 300 kb DNA a...

journal_title：Nucleic acids research

authors： Baas F,van Ommen GJ,Bikker H,Arnberg AC,de Vijlder JJ

更新日期：1986-07-11 00:00:00

abstract：:Precursor tRNAAsp molecules, containing a 26-base 5' leader, were treated with diethylpyrocarbonate, 50% hydrazine or anhydrous hydrazine/3M NaCl and then subjected to processing by RNase P RNAs from Escherichia coli or Bacillus subtilis. Fully processed tRNAs and material not successfully cleaved by the catalytic RNA...

journal_title：Nucleic acids research

authors： Thurlow DL,Shilowski D,Marsh TL

更新日期：1991-02-25 00:00:00

abstract：:The conserved bacterial anticodon nuclease (ACNase) RloC and its phage-excluding homolog PrrC comprise respective ABC-adenosine triphosphatase (ATPase) and ACNase N- and C-domains but differ in three key attributes. First, prrC is always linked to an ACNase silencing, DNA restriction-modification (R-M) locus while rlo...

journal_title：Nucleic acids research

authors： Klaiman D,Steinfels-Kohn E,Krutkina E,Davidov E,Kaufmann G

更新日期：2012-09-01 00:00:00

abstract：:Robust methods to detect DNA-binding proteins from structures of unknown function are important for structural biology. This paper describes a method for identifying such proteins that (i) have a solvent accessible structural motif necessary for DNA-binding and (ii) a positive electrostatic potential in the region of ...

journal_title：Nucleic acids research

authors： Shanahan HP,Garcia MA,Jones S,Thornton JM

更新日期：2004-09-08 00:00:00

abstract：:A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five 'pQLink' vectors for expression of His-tag and GST-tag fusion proteins as well as untagged proteins and for cloning by restriction en...

journal_title：Nucleic acids research

authors： Scheich C,Kümmel D,Soumailakakis D,Heinemann U,Büssow K

更新日期：2007-01-01 00:00:00

abstract：:Using a genetic selection for suppressors of a UGA nonsense mutation in trpA, we have isolated a G to A transition mutation at position 1491 in the decoding region of 16S rRNA. This suppressor displayed no codon specificity, suppressing UGA, UAG and UAA nonsense mutations and +1 and -1 frameshift mutations in lacZ. Su...

journal_title：Nucleic acids research

authors： Gregory ST,Dahlberg AE

更新日期：1995-11-11 00:00:00

abstract：:MicroRNAs (miRNAs) are small RNAs ∼22 nt in length that are involved in the regulation of a variety of physiological and pathological processes. Advances in high-throughput small RNA sequencing (smRNA-seq), one of the next-generation sequencing applications, have reshaped the miRNA research landscape. In this study, w...

journal_title：Nucleic acids research

authors： Cheng WC,Chung IF,Huang TS,Chang ST,Sun HJ,Tsai CF,Liang ML,Wong TT,Wang HW

更新日期：2013-01-01 00:00:00

abstract：:Understanding interactions between proteins and RNA is key to deciphering the mechanisms of many important biological processes. Here we describe RNABindR, a web-based server that identifies and displays RNA-binding residues in known protein-RNA complexes and predicts RNA-binding residues in proteins of unknown struct...

journal_title：Nucleic acids research

authors： Terribilini M,Sander JD,Lee JH,Zaback P,Jernigan RL,Honavar V,Dobbs D

更新日期：2007-07-01 00:00:00

abstract：:It has been postulated that a myriad of long noncoding RNAs (lncRNAs) contribute to gene regulation. In fission yeast, glucose starvation triggers lncRNA transcription across promoter regions of stress-responsive genes including fbp1 (fructose-1,6-bisphosphatase1). At the fbp1 promoter, this transcription promotes chr...

journal_title：Nucleic acids research

authors： Takemata N,Oda A,Yamada T,Galipon J,Miyoshi T,Suzuki Y,Sugano S,Hoffman CS,Hirota K,Ohta K

更新日期：2016-06-20 00:00:00

abstract：:Several proteins that have substantially diverged during evolution retain similar three-dimensional structures and biological function inspite of poor sequence identity. The database on Genomic Distribution of protein structural domain Superfamilies (GenDiS) provides record for the distribution of 4001 protein domains...

journal_title：Nucleic acids research

authors： Pugalenthi G,Bhaduri A,Sowdhamini R

更新日期：2005-01-01 00:00:00

abstract：:ID elements comprise a rodent SINE (short interspersed DNA repetitive element) family that has amplified by retroposition of a few master genes. In order to understand the important factors of SINE amplification, we investigated the transcription of rat ID elements. Three different size classes of ID transcripts, BC1,...

journal_title：Nucleic acids research

authors： Kim J,Kass DH,Deininger PL

更新日期：1995-06-25 00:00:00

abstract：:Although cis-regulatory binding sites (CRBSs) are at least as important as the coding sequences in a genome, our general understanding of them in most sequenced genomes is very limited due to the lack of efficient and accurate experimental and computational methods for their characterization, which has largely hindere...

journal_title：Nucleic acids research

authors： Zhang S,Xu M,Li S,Su Z

更新日期：2009-06-01 00:00:00

abstract：:The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...

journal_title：Nucleic acids research

authors： Ren Q,Au HH,Wang QS,Lee S,Jan E

更新日期：2014-08-01 00:00:00

abstract：:The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt ...

journal_title：Nucleic acids research

authors： Hartmann B,Thuong NT,Pouyet J,Ptak M,Leng M

更新日期：1983-07-11 00:00:00

abstract：:Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replication, but clinically useful inhibitors have not been developed. Here, we report an assay for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput screening and mechanistic studies. Initia...

journal_title：Nucleic acids research

authors： Culyba M,Hwang Y,Attar S,Madrid PB,Bupp J,Huryn D,Sanchez L,Grobler J,Miller MD,Bushman FD

更新日期：2012-09-01 00:00:00