Abstract:
:Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three germ layers, and the teratomas can be inspected through high-quality digital images. The teratoma assay, however, lacks consistency in transplantation protocols and even in interpretation, which needs community-based efforts for improving the assay quality. Here, we have developed a novel database OpenTein (Open Teratoma Investigation, http://opentein.hgc.jp/) to archive and freely distribute high-resolution whole-slide images and relevant records. OpenTein has been designed as a searchable, zoomable and annotatable web-based repository system. We have deposited 468 images of teratomas derived by our transplantation of human stem cells, and users can freely access and process such digital teratoma images. Approximately, the current version of OpenTein responds within 11.2 min for processing 2.03 gigapixel teratoma images. Our system offers valuable tools and resources in the new era of stem cell biology.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Park SJ,Komiyama Y,Suemori H,Umezawa A,Nakai Kdoi
10.1093/nar/gkv1096subject
Has Abstractpub_date
2016-01-04 00:00:00pages
D1000-4issue
D1eissn
0305-1048issn
1362-4962pii
gkv1096journal_volume
44pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
abstract::The COXPRESdb (http://coxpresdb.jp) provides gene coexpression relationships for animal species. Here, we report the updates of the database, mainly focusing on the following two points. For the first point, we added RNAseq-based gene coexpression data for three species (human, mouse and fly), and largely increased th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1163
更新日期:2015-01-01 00:00:00
abstract::A typical G-rich telomeric DNA strand, which runs 5'-->3' toward the chromosome ends, protrudes by several nucleotides in lower eukaryotes. In human chromosomes long G-rich 3'-overhangs have been found. Apart from the standard G-rich tail, several non-canonical terminal structures have been proposed. However, the mech...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2017-03-17 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw098
更新日期:2016-05-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.18.5465
更新日期:1990-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1050
更新日期:2019-01-08 00:00:00
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pub_type: 杂志文章
doi:10.1093/nar/14.1.31
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pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-07-09 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-06-15 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks004
更新日期:2012-05-01 00:00:00
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