Abstract:
:We characterize a component of the E. coli bacterial nucleoid H1a, which accumulates in stationary phase. This protein, identical with the major component of a plasmid-protein complex previously isolated in our laboratory, has a pI close to 7.5. Acrylamide gel electrophoresis and sedimentation in sucrose gradient have shown that the protein H1a induces significant compaction into DNA. This compaction is equivalent to that observed in nucleosome core although it introduces only a slight change in linking number. In addition, the structural change induced in the lactose L8UV5 promoter by H1a results in the decrease in the kinetic of formation of the open complex with RNA polymerase.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Spassky A,Rimsky S,Garreau H,Buc Hdoi
10.1093/nar/12.13.5321subject
Has Abstractpub_date
1984-07-11 00:00:00pages
5321-40issue
13eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::A class II site-specific endodeoxyribonuclease (SalPI) was identified in cell-free extracts of Streptomyces albus CMI 52766 after high speed centrifugation and fractionation through Bio Gel AO.5M. SalPI cleaves lambda DNA into at least 18 fragments. Five cleavage sites were located in the linear lambda map by the use ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.6.1989
更新日期:1977-06-01 00:00:00
abstract::To unravel the network of transcription factors established during development it is important to understand how genes specifically expressed during embryogenesis are regulated. Oct-4 is a transcription factor whose expression is associated with an undifferentiated cell phenotype in the early mouse embryo and is downr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.6.901
更新日期:1994-03-25 00:00:00
abstract::Heterochromatin protein 1 (HP1) is an evolutionarily conserved chromosomal protein that binds to lysine 9-methylated histone H3 (H3K9me), a hallmark of heterochromatin. Although HP1 phosphorylation has been described in several organisms, the biological implications of this modification remain largely elusive. Here we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku995
更新日期:2014-11-10 00:00:00
abstract::The dhp1+ gene of Schizosaccharomyces pombe is a homolog of Saccharomyces cerevisiae HKE1/RAT1/TAP1 gene that is involved in RNA metabolism such as RNA trafficking and RNA synthesis. dhp1+ is also related to S. cerevisiae DST2 (SEP1) that encodes a DNA strand exchange protein required for sporulation and homologous re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.3.357
更新日期:1995-02-11 00:00:00
abstract::The human mitochondrial translation apparatus, which synthesizes the core subunits of the oxidative phosphorylation system, is of central interest as mutations in several genes encoding for mitoribosomal proteins or translation factors cause severe human diseases. Little is known, how this complex machinery assembles ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky701
更新日期:2018-09-19 00:00:00
abstract::In neural stem cells (NSCs), the balance between stem cell maintenance and neuronal differentiation depends on cell-fate determinants such as TRIM32. Previously, we have shown that TRIM32 associates with the RNA-induced silencing complex and increases the activity of microRNAs such as Let-7a. However, the exact mechan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv138
更新日期:2015-03-11 00:00:00
abstract::The -1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome corona...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki731
更新日期:2005-07-29 00:00:00
abstract::DrugCentral (http://drugcentral.org) is an open-access online drug compendium. DrugCentral integrates structure, bioactivity, regulatory, pharmacologic actions and indications for active pharmaceutical ingredients approved by FDA and other regulatory agencies. Monitoring of regulatory agencies for new drugs approvals ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw993
更新日期:2017-01-04 00:00:00
abstract::RNA polymerase III recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo(rU) synthesis within this sequence, and releases the RNA. The pol III subunit Rpc11p (C11) mediates RNA 3'-5' cleavage in the catalytic center of pol III during pausing. The amino and carboxyl region...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr182
更新日期:2011-08-01 00:00:00
abstract::We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished without the introduction ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.5.1445
更新日期:1978-05-01 00:00:00
abstract::An alpha-neo-endorphin (alpha NE) gene, which we previously synthesized chemically and inserted into E. coli beta-galactosidase gene of pK013 plasmid, has been excised and fused to E. coli alkaline phosphatase (APase) gene. One of the transformants was named E15/pA alpha NE1. Under the APase gene regulation, APase-alp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.5.1283
更新日期:1983-03-11 00:00:00
abstract::A successful application is reported of the multivariate curve resolution alternating least-squares method (MCR-ALS) for the analysis of nucleic acid melting and salt-induced transitions. Under conditions where several structures co-exist in a conformational equilibrium, MCR-ALS analysis of the UV and circular dichroi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnf091
更新日期:2002-09-01 00:00:00
abstract::We report the synthesis of new phosphoramidite building blocks and their use for the modification of oligonucleotides with hydrazides. The reaction of these hydrazide oligonucleotides with active esters and aldehydes is demonstrated for solution conjugation and immobilization. Compared with the established amino modif...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf594
更新日期:2002-11-01 00:00:00
abstract::Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg560
更新日期:2003-07-01 00:00:00
abstract::The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1294
更新日期:2021-01-19 00:00:00
abstract::Eucaryotic ribosome biogenesis involves many cis-acting sequences and trans-acting factors, including snoRNAS: We have used directed mutagenesis of rDNA plasmids in yeast to identify critical sequence and structural elements within and flanking the ITS2-proximal stem. This base paired structure, present in the mature ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.10.2106
更新日期:2001-05-15 00:00:00
abstract::We have sequenced the entire human S mu and S gamma 4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S epsilon. S mu is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S epsilon at a much lower density. S mu-S gamma 4 ma...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7305
更新日期:1990-12-25 00:00:00
abstract::Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.10.3255
更新日期:1983-05-25 00:00:00
abstract::Eukaryotic elongation factor 1 A (eEF1A, formerly elongation factor-1 alpha) is an important component of the protein synthesis apparatus. Here we report the isolation and characterization of the cDNA sequence encoding rabbit eEF1A-2, an isoform of eEF1A, as well as a structural and functional comparison of the two ra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.8.1884
更新日期:1998-04-15 00:00:00
abstract::(19)F NMR spectroscopy has proved to be a valuable tool to monitor functionally important conformational transitions of nucleic acids. Here, we present a systematic investigation on the application of 5-fluoro pyrimidines to probe DNA and RNA secondary structures. Oligonucleotides with the propensity to adapt secondar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp862
更新日期:2009-12-01 00:00:00
abstract::BmFTZ-F1 is a Bombyx mori homologue of FTZ-F1, a positive regulator of the fushi tarazu gene of Drosophila melanogaster. In order to determine the sequence recognized with this factor, we made three sets of oligonucleotide mixture which contain 4 possible nucleotides at different positions within the previously propos...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.13.3689
更新日期:1991-07-11 00:00:00
abstract::A protein-DNA complex has less gel electrophoretic mobility than the free DNA fragment. One parameter for the degree of retardation of a linear DNA fragment in a protein-DNA complex is the molecular weight of the bound protein(s). The quotient of the migration distances of free DNA (m) and protein-DNA complex (m') is ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.12.5241
更新日期:1988-06-24 00:00:00
abstract::The proportion of cytosines methylated in the DNA of nucleosome oligomers and of core particles appears indistinguishable from that of total nuclear DNA from CHO cells. However the DNA in nucleoprotein which is initially released from nuclei by treatment with very low levels of micrococcal nuclease and the first 10% o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.9.3097
更新日期:1977-09-01 00:00:00
abstract::p53 as an effector of nucleolar stress is well defined, but p53 independent mechanisms are largely unknown. Like p53, the NF-κB transcription factor plays a critical role in maintaining cellular homeostasis under stress. Many stresses that stimulate NF-κB also disrupt nucleoli. However, the link between nucleolar func...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky455
更新日期:2018-07-06 00:00:00
abstract::Genomes mutate and evolve in ways simple (substitution or deletion of bases) and complex (e.g. chromosome shattering). We do not fully understand what types of complex mutation occur, and we cannot routinely characterize arbitrarily-complex mutations in a high-throughput, genome-wide manner. Long-read DNA sequencing m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1266
更新日期:2018-02-28 00:00:00
abstract::The database of imprinted genes and parent-of-origin effects in animals (http://www.otago.ac.nz/IGC ) is a collation of genes and phenotypes for which parent-of-origin effects have been reported. The database currently includes over 220 entries, which describe over 40 imprinted genes in human, mouse and other animals....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.275
更新日期:2001-01-01 00:00:00
abstract::DNA circuit is a versatile and highly-programmable toolbox which can potentially be used for the autonomous sensing of dynamic events, such as biomolecular interactions. However, the experimental implementation of in silico circuit designs has been hindered by the problem of circuit leakage. Here, we systematically an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw447
更新日期:2016-08-19 00:00:00
abstract::The PriA protein was identified in Escherichia coli as a factor involved in the replication of extrachromosomal elements such as bacteriophage phiX174 and plasmid pBR322. Recent data show that PriA plays an important role in chromosomal replication, by promoting reassembly of the replication machinery during reinitiat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.7.1593
更新日期:2002-04-01 00:00:00
abstract::Multiplexed high-throughput pyrosequencing is currently limited in complexity (number of samples sequenced in parallel), and in capacity (number of sequences obtained per sample). Physical-space segregation of the sequencing platform into a fixed number of channels allows limited multiplexing, but obscures available s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm760
更新日期:2007-01-01 00:00:00
abstract::The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5469
更新日期:1981-10-24 00:00:00