Abstract：
:We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished without the introduction of detectable scissions away from the ends of the duplexes. When this nuclease is used to produce a series of progressively shortened samples from a linear duplex DNA, subsequent digestion of these samples with a site-specific restriction endonuclease and analysis of the resulting fragments by gel electrophoresis permits the rapid establishment of the order of the restriction enzyme fragments through the entire genome. This is accomplished by noting from the electropherograms the order in which the various restriction enzyme fragments become noticeably shortened or disappear. Using this method, the five cleavage sites for the endonuclease Hpa I and the single cleavage sites for the nucleases Hpa II and Pst I have been mapped in PM2 bacteriophage DNA. In a more stringent test of the method, 18 of the 24 fragments produced by cleavage of coliphage lambdab2b5c DNA with the Pst I nuclease have been mapped, and five of the six remaining fragments have been assigned to small regions of the genome.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Legerski RJ,Hodnett JL,Gray HB Jrdoi
10.1093/nar/5.5.1445subject
Has Abstractpub_date
1978-05-01 00:00:00pages
1445-64issue
5eissn
0305-1048issn
1362-4962journal_volume
5pub_type
杂志文章abstract：:The transactivator responsive region (TAR) present in the 5'-NTR of the HIV-1 genome represents a potential target for antiretroviral intervention and a model system for the development of specific inhibitors of RNA-protein interaction. Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA(TAR)) con...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki743
更新日期：2005-08-02 00:00:00
abstract：:We have previously investigated the role of the N-terminal region of ribosomal protein S4 to participate in 30S ribosome assembly and function (1-3). In this report we extend these studies to the two fragments produced by the chemical cleavage of protein S4 at the tryptophan residue 167. We find that the carboxyl term...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.17.6343
更新日期：1985-09-11 00:00:00
abstract：:The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variabl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.12.2777
更新日期：1981-06-25 00:00:00
abstract：:The polypeptides recognized by a monoclonal antibody against calf thymus DNA polymerase alpha (secreted from a hybridoma CL22 -2- 42B , Nucleic Acids Res. (1982) 10, 4703-4713) were identified by the immunoblot method as the large polypeptides of the partially-purified 10S DNA polymerase alpha fraction. Using an immun...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.11.4455
更新日期：1984-06-11 00:00:00
abstract：:RNA secondary structures, e.g. stem-loops that are often found at the 5' and 3' ends of mRNAs, are in many cases known to be crucial for transcript stability but their role in prolonging the lifetime of transcripts remains elusive. In this study we show for an essential RNA-stabilizing stem-loop at the 5' end of rbcL ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki760
更新日期：2005-08-22 00:00:00
abstract：:Many nucleic acid-binding proteins and the AAA+ family form hexameric rings, but the mechanism of hexamer assembly is unclear. It is generally believed that the specificity in protein/RNA interaction relies on molecular contact through a surface charge or 3D structure matching via conformational capture or induced fit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn669
更新日期：2008-11-01 00:00:00
abstract：:The genome-wide identification of microRNA transcription start sites (miRNA TSSs) is essential for understanding how miRNAs are regulated in development and disease. In this study, we developed mirSTP (mirna transcription Start sites Tracking Program), a probabilistic model for identifying active miRNA TSSs from nasce...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx318
更新日期：2017-07-27 00:00:00
abstract：:The nucleotide sequences of the 5S rRNAs of the oomycete water molds Saprolegnia ferax and Pythium hydnosporum and of the chytrid water molds Blastocladiella simplex and Phlyctochytrium irregulare were determined by chemical and enzymatic partial degradation of 3' and 5' end-labelled molecules, followed by gel sequenc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.18.5717
更新日期：1982-09-25 00:00:00
abstract：:Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.1.327
更新日期：1988-01-11 00:00:00
abstract：:Protein-protein and protein-DNA/RNA interactions play a fundamental role in a variety of biological processes. Determining the complex structures of these interactions is valuable, in which molecular docking has played an important role. To automatically make use of the binding information from the PDB in docking, her...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx407
更新日期：2017-07-03 00:00:00
abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.14.4793
更新日期：1983-07-25 00:00:00
abstract：:Influenza viruses have a segmented viral RNA (vRNA) genome, which is replicated by the viral RNA-dependent RNA polymerase (RNAP). Replication initiates on the vRNA 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the synthesis of new vRNA. RNAP structures show the 3' termi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw884
更新日期：2016-12-01 00:00:00
abstract：:Long terminal repeat (LTR)-retrotransposons comprise a significant portion of the rice genome. Their complete characterization is thus necessary if the sequenced genome is to be annotated correctly. In addition, because LTR-retrotransposons can influence the expression of neighboring genes, the complete identification...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl780
更新日期：2007-01-01 00:00:00
abstract：:These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and Cfo I, are reduced with Z DNA as compared to B DNA. The abilit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.18.7237
更新日期：1986-09-25 00:00:00
abstract：:Among a collection of 74 aprt mutations induced by treatment of plateau phase Chinese hamster ovary CHO cells with the radiomimetic DNA double-strand cleaving agent neocarzinostatin, nine were large-scale rearrangements. Molecular analysis indicated that all nine were highly conservative, non-homologous reciprocal exc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf369
更新日期：2002-06-15 00:00:00
abstract：:Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alteration...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.3.883
更新日期：1988-02-11 00:00:00
abstract：:An efficient chemical procedure for the immobilization of carboxylate containing conjugate groups onto controlled pore glass (CPG) is described. The derivatized supports were used in the automated synthesis of an oligodeoxynucleotide (20-mer ODN) containing a 3' phosphodiester linked hexanol, aminohexyl, acridine, or ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.1.145
更新日期：1993-01-11 00:00:00
abstract：:RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular u...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.12.2311
更新日期：1997-06-15 00:00:00
abstract：:In silico prediction of transcription factor binding sites (TFBSs) is central to the task of gene regulatory network elucidation. Genomic DNA sequence information provides a basis for these predictions, due to the sequence specificity of TF-binding events. However, DNA sequence alone is an impoverished source of infor...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn866
更新日期：2009-01-01 00:00:00
abstract：:The nucleoplasm is not homogenous; it consists of many types of nuclear bodies, also known as nuclear domains or nuclear subcompartments. These self-organizing structures gather machinery involved in various nuclear activities. Nuclear speckles (NSs) or splicing speckles, also called interchromatin granule clusters, w...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkx759
更新日期：2017-10-13 00:00:00
abstract：:We have developed an HIV nef-Escherichia coli lacZ fusion system in vitro that allows the detection of low frequency mutations, including frameshifts, deletions and insertions. A portion of the nef gene that encompasses a hypervariable region was fused in-frame with a downstream lacZalpha peptide coding region. The re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.9.1710
更新日期：1996-05-01 00:00:00
abstract：:Increasing experimental evidence shows a prominent role of histone modifications in the coordinated control of gene expression in the human malaria parasite Plasmodium falciparum. The search for the histone-mark-reading machinery that translates histone modifications into biological processes, such as formation of het...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp115
更新日期：2009-05-01 00:00:00
abstract：:Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.16.5015
更新日期：1982-08-25 00:00:00
abstract：:Ribonucleoprotein (RNP) granules are membraneless organelles (MLOs), which majorly consist of RNA and RNA-binding proteins and are formed via liquid-liquid phase separation (LLPS). Experimental studies investigating the drivers of LLPS have shown that intrinsically disordered proteins (IDPs) and nucleic acids like RNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1099
更新日期：2020-12-16 00:00:00
abstract：:A new restriction endonuclease Bse SI has been isolated from Bacillus stearothermophilus Jo10-553. Bse SI recognizes a degenerate hexanucleotide sequence 5'-G(G/T)GC(A/C)C-3' and cleaves DNA to produce 3[prime]-protruding tetranucleotide ends. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.13.2644
更新日期：1999-07-01 00:00:00
abstract：:We have isolated an eight kilobase fragment of Bacillus subtilis DNA by specific integration and excision of a plasmid containing a sequence adjacent to ribosomal operon rrn O. The genetic locus of the cloned fragment was verified by linkage of the integrated vector to nearby genetic markers using both transduction an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.15.6307
更新日期：1984-08-10 00:00:00
abstract：:TFIIIC is a RNA polymerase (pol) III-specific DNA-binding factor that is required for transcription of tRNA and 5S rRNA genes. Active human TFIIIC consists of five subunits. However, an inactive form has also been isolated that lacks one of the five subunits, called TFIIIC110. A model was proposed in which pol III tra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl432
更新日期：2006-07-05 00:00:00
abstract：:Slime mold, plant and insect dihydropyrimidine amidohydrolases (DHPases, EC 3.5.2.2), which catalyze the second step of pyrimidine and several anti-cancer drug degradations, were cloned and shown to functionally replace a defective DHPase enzyme in the yeast Saccharomyces kluyveri. The yeast and slime mold DHPases wer...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg258
更新日期：2003-03-15 00:00:00
abstract：:Multi-modal interactions are frequently observed in intrinsically disordered regions (IDRs) of proteins upon binding to their partners. In many cases, post-translational modifications in IDRs are accompanied by coupled folding and binding. From both molecular simulations and biochemical experiments with mutational stu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1297
更新日期：2018-03-16 00:00:00
abstract：:Argonaute (AGO) proteins have a well-established role in post-transcriptional regulation of gene expression as key component of the RNA silencing pathways. Recent evidence involves AGO proteins in mammalian nuclear processes such as transcription and splicing, though the mechanistic aspects of AGO nuclear functions re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1387
更新日期：2015-02-18 00:00:00
