Plasmodium falciparum heterochromatin protein 1 binds to tri-methylated histone 3 lysine 9 and is linked to mutually exclusive expression of var genes.

abstract：:Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...

journal_title：Nucleic acids research

authors： Sørensen CS,Syljuåsen RG

更新日期：2012-01-01 00:00:00

abstract：:The database of 3D interacting domains (3did, available online for browsing and bulk download at http://3did.irbbarcelona.org) is a catalog of protein-protein interactions for which a high-resolution 3D structure is known. 3did collects and classifies all structural templates of domain-domain interactions in the Prote...

journal_title：Nucleic acids research

authors： Mosca R,Céol A,Stein A,Olivella R,Aloy P

更新日期：2014-01-01 00:00:00

abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...

journal_title：Nucleic acids research

authors： Burel SA,Hart CE,Cauntay P,Hsiao J,Machemer T,Katz M,Watt A,Bui HH,Younis H,Sabripour M,Freier SM,Hung G,Dan A,Prakash TP,Seth PP,Swayze EE,Bennett CF,Crooke ST,Henry SP

更新日期：2016-03-18 00:00:00

abstract：:The location of proteins on the mitochondrial DNA (mtDNA) of Drosophila virilis was investigated by Me3 psoralen photoreaction of mitochondria isolated from embryos. After photoreaction the mtDNA was purified and the pattern of DNA cross-linking was determined by electron microscopy of the DNA under totally denaturing...

journal_title：Nucleic acids research

authors： Pardue ML,Fostel JM,Cech TR

更新日期：1984-02-24 00:00:00

abstract：:Methylation of the 2'-hydroxyl-group of ribonucleotides is found in all major classes of RNA in eukaryotes and is one of the most abundant posttranscriptional modifications of stable RNAs. In spite of intense studies, the multiple functions of RNA 2'-O-methylation are still not understood. One major obstacle in the fi...

journal_title：Nucleic acids research

authors： Aschenbrenner J,Marx A

更新日期：2016-05-05 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA...

journal_title：Nucleic acids research

authors： Zimmermann RM,Cox EC

更新日期：1994-02-11 00:00:00

abstract：:Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimer...

journal_title：Nucleic acids research

authors： Sciabica KS,Hertel KJ

更新日期：2006-01-01 00:00:00

abstract：:The Rhizobium trifolii nod genes required for host-specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer e...

journal_title：Nucleic acids research

authors： Schofield PR,Watson JM

更新日期：1986-04-11 00:00:00

abstract：:In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attr...

journal_title：Nucleic acids research

authors： Rahn RO,Schleich T

更新日期：1974-08-01 00:00:00

abstract：:We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...

journal_title：Nucleic acids research

authors： Colantuoni V,Romano V,Bensi G,Santoro C,Costanzo F,Raugei G,Cortese R

更新日期：1983-11-25 00:00:00

abstract：:Rice alpha-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene,...

journal_title：Nucleic acids research

authors： Huang N,Koizumi N,Reinl S,Rodriguez RL

更新日期：1990-12-11 00:00:00

abstract：:To generate long arrays of nucleosomes within a topologically defined chromatin domain we have assembled minichromosomes on negatively supercoiled plasmid DNA with extracts from Drosophila preblastoderm embryos. These minichromosomes are dynamic substrates for energy-dependent nucleosome remodeling machines that facil...

journal_title：Nucleic acids research

authors： Venditti P,Di Croce L,Kauer M,Blank T,Becker PB,Beato M

更新日期：1998-08-15 00:00:00

abstract：:Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both ...

journal_title：Nucleic acids research

authors： Kasai H,Shindo-Okada N,Noguchi S,Nishimura S

更新日期：1979-09-11 00:00:00

abstract：:Radioactive iodine has been used to probe the relative reactivities of nucleosomal H4 tyrosine residues under various conditions of subphysiological ionic strength. We observe that tyrosine 72 of H4, which is not reactive over the range 20-150 mM NaCl, becomes the predominant site of iodination within H4 when nucleoso...

journal_title：Nucleic acids research

authors： Burch JB,Martinson HG

更新日期：1981-09-11 00:00:00

abstract：:Mitochondrial DNA (mtDNA) contains high levels of oxidative damage relative to nuclear DNA. A full, functional DNA base excision repair (BER) pathway is present in mitochondria, to repair oxidative DNA lesions. However, little is known about the organization of this pathway within mitochondria. Here, we provide eviden...

journal_title：Nucleic acids research

authors： Stuart JA,Mayard S,Hashiguchi K,Souza-Pinto NC,Bohr VA

更新日期：2005-07-08 00:00:00

abstract：:The surface accessibility of macromolecules plays a key role in modulating molecular recognition events. RNA is a complex and dynamic molecule involved in many aspects of gene expression. However, there are few experimental methods available to measure the accessible surface of RNA. Here, we investigate the accessible...

journal_title：Nucleic acids research

authors： Venditti V,Niccolai N,Butcher SE

更新日期：2008-03-01 00:00:00

abstract：:Human immunodeficiency virus type 2 (HIV-2) reverse transcription is initiated from cellular tRNA(Lys3) partially annealed to the RNA viral genome at the primer binding site (PBS). This annealing involves interactions between two highly structured RNA molecules. In contrast to HIV-1, in which the reverse transcription...

journal_title：Nucleic acids research

authors： Freund F,Boulmé F,Litvak S,Tarrago-Litvak L

更新日期：2001-07-01 00:00:00

abstract：:Yeast tRNA Val 1 alkylation with 2', 3'-O-4-(N-2-chloroethyl-N-methylamino) benzylidene d(pC-G)-A proceeds at 20 degrees - 30 degrees C in the complementary complexes which are formed by d(pC-G)-A greater than RC1 binding to 3 sequences of tRNA Val 1 : psi-C-G58 in the T loop, C-G40 at the 3'-side of the anticodon loo...

journal_title：Nucleic acids research

authors： Grineva NI,Karpova GG,Kuznetsova LM,Venkstern TV,Bayev AA

更新日期：1977-01-01 00:00:00

abstract：:Genome-wide association (GWA) studies to map genes for complex traits are powerful yet costly. DNA-pooling strategies have the potential to dramatically reduce the cost of GWA studies. Pooling using Affymetrix arrays has been proposed and used but the efficiency of these arrays has not been quantified. We compared and...

journal_title：Nucleic acids research

authors： Macgregor S,Zhao ZZ,Henders A,Nicholas MG,Montgomery GW,Visscher PM

更新日期：2008-04-01 00:00:00

abstract：:Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, w...

journal_title：Nucleic acids research

authors： Lucas I,Hyrien O

更新日期：2000-05-15 00:00:00

abstract：:Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D88N) of RecU from Bacillus subtilis in complex with a 12 base palindromic DNA fragment at a resolution of 3.2 Å. This structure shows the stalk region and the essentia...

journal_title：Nucleic acids research

authors： Khavnekar S,Dantu SC,Sedelnikova S,Ayora S,Rafferty J,Kale A

更新日期：2017-01-25 00:00:00

abstract：:Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...

journal_title：Nucleic acids research

authors： Grocott T,Frost V,Maillard M,Johansen T,Wheeler GN,Dawes LJ,Wormstone IM,Chantry A

更新日期：2007-01-01 00:00:00

abstract：:To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

journal_title：Nucleic acids research

authors： Heuchel R,Matthias P,Schaffner W

更新日期：1989-11-25 00:00:00

abstract：:Replication protein A (RPA) plays a critical role in all eukaryotic DNA processing involving single-stranded DNA (ssDNA). Contrary to the notion that RPA provides solely inert protection to transiently formed ssDNA, the RPA-ssDNA complex acts as a dynamic DNA processing unit. Here, we studied the diffusion of RPA alon...

journal_title：Nucleic acids research

authors： Mishra G,Bigman LS,Levy Y

更新日期：2020-02-28 00:00:00

abstract：:To date, an effective therapeutic treatment that confers strong attenuation toward coronaviruses (CoVs) remains elusive. Of all the potential drug targets, the helicase of CoVs is considered to be one of the most important. Here, we first present the structure of the full-length Nsp13 helicase of SARS-CoV (SARS-Nsp13)...

journal_title：Nucleic acids research

authors： Jia Z,Yan L,Ren Z,Wu L,Wang J,Guo J,Zheng L,Ming Z,Zhang L,Lou Z,Rao Z

更新日期：2019-07-09 00:00:00

abstract：:Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded DNA from nucleolytic attack, preventing intra-strand pairing events and playing many other regulatory roles within the replisome. Recent developments in single-molecule approaches have led to a revised picture of the repl...

journal_title：Nucleic acids research

authors： Spenkelink LM,Lewis JS,Jergic S,Xu ZQ,Robinson A,Dixon NE,van Oijen AM

更新日期：2019-05-07 00:00:00

abstract：:The Bovine Genome Database (BGD; http://BovineGenome.org) strives to improve annotation of the bovine genome and to integrate the genome sequence with other genomics data. BGD includes GBrowse genome browsers, the Apollo Annotation Editor, a quantitative trait loci (QTL) viewer, BLAST databases and gene pages. Genome ...

journal_title：Nucleic acids research

authors： Childers CP,Reese JT,Sundaram JP,Vile DC,Dickens CM,Childs KL,Salih H,Bennett AK,Hagen DE,Adelson DL,Elsik CG

更新日期：2011-01-01 00:00:00

abstract：:The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between three proteins microscopically. Flow cytometry offers excellent sensitivity, effective signal separation and the capacity to assess a...

journal_title：Nucleic acids research

authors： He L,Wu X,Simone J,Hewgill D,Lipsky PE

更新日期：2005-04-01 00:00:00

abstract：:The stable intermediates formed in the reaction of cytosine, cytidine and adenosine with chloracetaldehyde were isolated. The -CH2CH/OH/- bridge between the exo and endo nitrogen atoms of the parent base was found in these compounds by means of PMR spectroscopy. Their acid-induced dehydration resulted in formation of ...

journal_title：Nucleic acids research

authors： Biernat J,Ciesiołka J,Górnicki P,Adamiak RW,Kryzosiak WJ,Wiewiórowski M

更新日期：1978-03-01 00:00:00