Bovine Genome Database: integrated tools for genome annotation and discovery.

abstract：:Compounds that are covalent analogues of nucleic acid base pairs of normal, long, and short C1' to C1' dimensions [B. Devadas and N.J. Leonard (1990) J. Am. Chem. Soc., 112, 3125-3135.] have been added to the oligodeoxyribonucleotide d(A)6 with bacteriophage T4 RNA ligase as a prelude to placing them at defined loci w...

journal_title：Nucleic acids research

authors： Petric A,Bhat B,Leonard NJ,Gumport RI

更新日期：1991-02-11 00:00:00

abstract：:Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...

journal_title：Nucleic acids research

authors： Hefel A,Honda M,Cronin N,Harrell K,Patel P,Spies M,Smolikove S

更新日期：2021-01-21 00:00:00

abstract：:We have used photo-crosslinking to investigate the structure and dynamics of four-way junction hairpin ribozyme constructs. Four phenylazide photo-crosslinkers were coupled to 2'-NH2-modified U+2 in the substrate and irradiated at different Mg2+ concentrations and temperatures. Consistent with the role of divalent met...

journal_title：Nucleic acids research

authors： Borda EJ,Sigurdsson ST

更新日期：2005-02-18 00:00:00

abstract：:Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...

journal_title：Nucleic acids research

authors： Yeoh LM,Goodman CD,Hall NE,van Dooren GG,McFadden GI,Ralph SA

更新日期：2015-05-19 00:00:00

abstract：:A hallmark of defense mechanisms based on clustered regularly interspaced short palindromic repeats (CRISPR) and associated sequences (Cas) are the crRNAs that guide these complexes in the destruction of invading DNA or RNA. Three separate CRISPR-Cas systems exist in the cyanobacterium Synechocystis sp. PCC 6803. Base...

journal_title：Nucleic acids research

authors： Reimann V,Alkhnbashi OS,Saunders SJ,Scholz I,Hein S,Backofen R,Hess WR

更新日期：2017-01-25 00:00:00

abstract：:Plant viruses ubiquitously mediate the induction of miR168 trough the activities of viral suppressors of RNA silencing (VSRs) controlling the accumulation of ARGONAUTE1 (AGO1), one of the main components of RNA silencing based host defence system. Here we used a mutant Tombusvirus p19 VSR (p19-3M) disabled in its main...

journal_title：Nucleic acids research

authors： Várallyay É,Oláh E,Havelda Z

更新日期：2014-01-01 00:00:00

abstract：:The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonst...

journal_title：Nucleic acids research

authors： Brou C,Wu J,Ali S,Scheer E,Lang C,Davidson I,Chambon P,Tora L

更新日期：1993-01-11 00:00:00

abstract：:MiRNAs are important regulators of gene expression and are frequently deregulated under pathologic conditions. They are highly stable in bodily fluids which makes them feasible candidates to become minimally invasive biomarkers. In fact, several studies already proposed circulating miRNA-based biomarkers for different...

journal_title：Nucleic acids research

authors： Aparicio-Puerta E,Jáspez D,Lebrón R,Koppers-Lalic D,Marchal JA,Hackenberg M

更新日期：2019-01-08 00:00:00

abstract：:Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specifi...

journal_title：Nucleic acids research

authors： Putnam WC,Daniher AT,Trawick BN,Bashkin JK

更新日期：2001-05-15 00:00:00

abstract：:Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...

journal_title：Nucleic acids research

authors： Tsutsui K,Watanabe S,Katagiri M,Oda T

更新日期：1983-07-25 00:00:00

abstract：:Post-transcriptional modifications are characteristic features of tRNAs and have been shown in a number of cases to influence both their structural and functional properties, including structure stabilization, amino-acylation and codon recognition. We have developed an approach which allows the investigation of the po...

journal_title：Nucleic acids research

authors： Helm M,Florentz C,Chomyn A,Attardi G

更新日期：1999-02-01 00:00:00

abstract：:We have previously shown that Y box-binding protein-1 (YB-1) binds preferentially to cisplatin-modified Y box sequences. Based on structural and biochemical data, we predicted that this protein binds single-stranded nucleic acids. In the present study we confirmed the prediction and also discovered some unexpected fun...

journal_title：Nucleic acids research

authors： Izumi H,Imamura T,Nagatani G,Ise T,Murakami T,Uramoto H,Torigoe T,Ishiguchi H,Yoshida Y,Nomoto M,Okamoto T,Uchiumi T,Kuwano M,Funa K,Kohno K

更新日期：2001-03-01 00:00:00

abstract：:Natural oligopeptides may regulate nearly all vital processes. To date, the chemical structures of nearly 6000 oligopeptides have been identified from >1000 organisms representing all the biological kingdoms. We have compiled the known physical, chemical and biological properties of these oligopeptides--whether synthe...

journal_title：Nucleic acids research

authors： Zamyatnin AA,Borchikov AS,Vladimirov MG,Voronina OL

更新日期：2006-01-01 00:00:00

abstract：:Gene II protein is the only phage-encoded protein required for the double-stranded DNA replication of the distantly related filamentous phages IKe and Ff (M13, fd and f1). Complementation studies have demonstrated that, despite a significant degree of homology between the nucleotide sequences of the gene II's of IKe a...

journal_title：Nucleic acids research

authors： Peeters BP,Schoenmakers JG,Konings RN

更新日期：1986-06-25 00:00:00

abstract：:RNA helicases of the DEAD-box protein family form the largest group of helicases. The human DEAD-box protein 1 (DDX1) plays an important role in tRNA and mRNA processing, is involved in tumor progression and is also hijacked by several virus families such as HIV-1 for replication and nuclear export. Although important...

journal_title：Nucleic acids research

authors： Kellner JN,Reinstein J,Meinhart A

更新日期：2015-03-11 00:00:00

abstract：:Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and...

journal_title：Nucleic acids research

authors： Finn PJ,Gibson NJ,Fallon R,Hamilton A,Brown T

更新日期：1996-09-01 00:00:00

abstract：:Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meio...

journal_title：Nucleic acids research

authors： Cheng Z,Brar GA

更新日期：2019-06-04 00:00:00

abstract：:Sequencing of RNAs (RNA-Seq) has revolutionized the field of transcriptomics, but the reads obtained often contain errors. Read error correction can have a large impact on our ability to accurately assemble transcripts. This is especially true for de novo transcriptome analysis, where a reference genome is not availab...

journal_title：Nucleic acids research

authors： Le HS,Schulz MH,McCauley BM,Hinman VF,Bar-Joseph Z

更新日期：2013-05-01 00:00:00

abstract：:Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but ...

journal_title：Nucleic acids research

authors： Sen GC,Ghosh HP

更新日期：1976-03-01 00:00:00

abstract：:We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...

journal_title：Nucleic acids research

authors： Potrykus K,Murphy H,Chen X,Epstein JA,Cashel M

更新日期：2010-03-01 00:00:00

abstract：:NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided. Drosophila NELF has four subunits similar to subunits of human NELF. The amino acid sequences ...

journal_title：Nucleic acids research

authors： Wu CH,Lee C,Fan R,Smith MJ,Yamaguchi Y,Handa H,Gilmour DS

更新日期：2005-03-01 00:00:00

abstract：:Murine erythroleukemic cells in culture accumulate cytoplasmic globin mRNA during differentiation induced by dimethyl sulfoxide (DMSO)1. Chromatin was prepared from DMSO induced erythroleukemic cells that were transcribing globin RNA in order to determine whether in vitro synthesis of globin RNA sequences was possible...

journal_title：Nucleic acids research

authors： Reff ME,Davidson RL

更新日期：1979-01-01 00:00:00

abstract：:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, h...

journal_title：Nucleic acids research

authors： Fukumura K,Taniguchi I,Sakamoto H,Ohno M,Inoue K

更新日期：2009-04-01 00:00:00

abstract：:An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher or...

journal_title：Nucleic acids research

authors： Barciszewski J,Bratek-Wiewiórowska MD,Górnicki P,Naskret-Barciszewska M,Wiewiórowski M,Zielenkiewicz A,Zielenkiewicz W

更新日期：1988-01-25 00:00:00

abstract：:The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...

journal_title：Nucleic acids research

authors： Shafer RW,Jung DR,Betts BJ,Xi Y,Gonzales MJ

更新日期：2000-01-01 00:00:00

abstract：:Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...

journal_title：Nucleic acids research

authors： Lemaigre FP,Ace CI,Green MR

更新日期：1993-06-25 00:00:00

abstract：:Ten transgenic mouse lines harboring the -346/-103 fragment of the rat insulin I enhancer linked to a heterologous promoter and a reporter gene (Eins-Ptk-CAT construct) were produced. Expression of the hybrid transgene was essentially observed in pancreas and to a lesser extent in brain. These results indicate that th...

journal_title：Nucleic acids research

authors： Dandoy-Dron F,Monthioux E,Jami J,Bucchini D

更新日期：1991-09-25 00:00:00

abstract：:Nuclear DNA from liver cells of the kangaroo rat species Dipodomysordii was fractionated and characterized with the aid of buoyant density gradients in neutral and alkaline CsCl and in Ag(+)-Cs(2)SO(4). More than one-half of the DNA was present in three density satellites, a greater proportion than in any other specie...

journal_title：Nucleic acids research

authors： Hatch FT,Mazrimas JA

更新日期：1974-04-01 00:00:00

abstract：:The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5' to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DN...

journal_title：Nucleic acids research

authors： Waltz SE,Trivedi AA,Leffak M

更新日期：1996-05-15 00:00:00

abstract：:Different pre-ribosomal complexes are formed during ribosome biogenesis, and the composition of these complexes is highly dynamic. Dbp4, a conserved DEAD-box RNA helicase implicated in ribosome biogenesis, interacts with nucleolar proteins Bfr2 and Enp2. We show that, like Dbp4, Bfr2 and Enp2 are required for the earl...

journal_title：Nucleic acids research

authors： Soltanieh S,Lapensée M,Dragon F

更新日期：2014-03-01 00:00:00