Abstract:
:Compounds that are covalent analogues of nucleic acid base pairs of normal, long, and short C1' to C1' dimensions [B. Devadas and N.J. Leonard (1990) J. Am. Chem. Soc., 112, 3125-3135.] have been added to the oligodeoxyribonucleotide d(A)6 with bacteriophage T4 RNA ligase as a prelude to placing them at defined loci within nucleic acid duplexes. Analogue cross sections that represent a normal Watson-Crick base pair as well as a pyrimidine-pyrimidine and a purine-purine apposition were ligated in modest yields (approximately 20%) to the oligonucleotide. Ligation conditions were optimized for each analogue, and the cross section was joined to only a single oligonucleotide in each case. The structures of the ligated products were proved by HPLC, enzymatic degradation, and spectroscopic analyses.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Petric A,Bhat B,Leonard NJ,Gumport RIdoi
10.1093/nar/19.3.585subject
Has Abstractpub_date
1991-02-11 00:00:00pages
585-90issue
3eissn
0305-1048issn
1362-4962journal_volume
19pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2014-07-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2007-01-01 00:00:00
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