Abstract:
:Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but with a much reduced efficiency. Although the modified tRNA Lys2 was recognized by elongation factor (EF) T, the EFT dependent binding to ribosomes to tRNA Lys2 (CNBr) was markedly decreased.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sen GC,Ghosh HPdoi
10.1093/nar/3.3.523subject
Has Abstractpub_date
1976-03-01 00:00:00pages
523-35issue
3eissn
0305-1048issn
1362-4962journal_volume
3pub_type
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1989-02-25 00:00:00
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更新日期:1989-07-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-10-01 00:00:00
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更新日期:2011-12-01 00:00:00
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