Abstract:
:The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonstrate that a factor(s) required for the function of the ER AFs is chromatographically separable from a factor(s) necessary for the activity of the acidic activation domain of VP16. Moreover, immunoprecipitation experiments using a monoclonal antibody directed against the TATA box binding protein (TBP) indicate, that these different factors are associated with TBP in distinct TFIID complexes.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brou C,Wu J,Ali S,Scheer E,Lang C,Davidson I,Chambon P,Tora Ldoi
10.1093/nar/21.1.5subject
Has Abstractpub_date
1993-01-11 00:00:00pages
5-12issue
1eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Message-specific translational regulation mechanisms shape the biogenesis of multimeric oxidative phosphorylation (OXPHOS) enzyme in mitochondria from the yeast Saccharomyces cerevisiae. These mechanisms, driven mainly by the action of mRNA-specific translational activators, help to coordinate synthesis of OXPHOS cata...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz266
更新日期:2019-06-20 00:00:00
abstract::YdiV is a negative regulator of cell motility. It interacts with FlhD(4)C(2) complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV(2)-FlhD(2) complex at 1.9 Å and 2.9 Å resolutions, respectively....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks869
更新日期:2012-11-01 00:00:00
abstract::We present the sequences of five additional human U2 pseudogenes which are very similar to the U2.13 pseudogene reported previously [Van Arsdell et al. (1981) Cell 26, 11-17]. All six U2 pseudogenes preserve the 5' end of the mature U2 snRNA sequence, and all six are flanked by nearly perfect direct repeats that diffe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.3.1463
更新日期:1984-02-10 00:00:00
abstract::Calf thymus DNA polymerase alpha (pol alpha) and bacteriophage T4 DNA polymerase (pol T4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butyl-anilino)dATP (BuAdATP) on the BuPdNTP-susceptible alpha polymerase family. Kinetic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1627
更新日期:1991-04-11 00:00:00
abstract::Chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic DNA duplexes by salt-gradient dialysis at various protein/DNA ratios. The resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. Electron...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3603
更新日期:1978-10-01 00:00:00
abstract::We have constructed vectors from bacteriophage lambda and from plasmid pBR322 having a single EcoRI restriction site which is immediately downstream from the lac UV5 promotor. Each vector allows the fusion of a cloned gene to the lac Z gene in a different phase relative to the translation initiation codon of the lac Z...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.12.4479
更新日期:1978-12-01 00:00:00
abstract::SPRING (http://algorithm.cs.nthu.edu.tw/tools/SPRING/) is a tool for the analysis of genome rearrangement between two chromosomal genomes using reversals and/or block-interchanges. SPRING takes two or more chromosomes as its input and then computes a minimum series of reversals and/or block-interchanges between any tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl169
更新日期:2006-07-01 00:00:00
abstract::HinP1I recognizes and cleaves the palindromic tetranucleotide sequence G downward arrowCGC in DNA. We report three structures of HinP1I-DNA complexes: in the presence of Ca(2+) (pre-reactive complex), in the absence of metal ion (binary complex) and in the presence of Mg(2+) (post-reactive complex). HinP1I forms a bac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj484
更新日期:2006-02-09 00:00:00
abstract::The base excision repair DNA glycosylase MutY homolog (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro-8-oxo-guanine (8-oxoG), thereby preventing G:C to T:A mutations. Biallelic germline mutations in the human MYH gene predispose individuals to multiple colorectal ade...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki209
更新日期:2005-01-26 00:00:00
abstract::We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves of Digitaria setigera. This study has confirmed that both spliced and unspliced RNAs are synthesised by the same transcription unit. The position of the intron...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7259
更新日期:1990-12-25 00:00:00
abstract::REV1 and DNA Polymerase ζ (REV3 and REV7) play important roles in translesion DNA synthesis (TLS) in which DNA replication bypasses blocking lesions. REV1 and Polζ have also been implicated in promoting repair of DNA double-stranded breaks (DSBs). However, the mechanism by which these two TLS polymerases increase tole...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr769
更新日期:2012-01-01 00:00:00
abstract::Loading of the replicative ring helicase onto the origin of replication (oriC) is the final outcome of a well coordinated series of events that collectively constitute a primosomal cascade. Once the ring helicase is loaded, it recruits the primase and signals the switch to the polymerization mode. The transient nature...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh628
更新日期:2004-06-01 00:00:00
abstract::Mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (PLRV) P1 (ORF1) protein were applied to the analysis of P1 expression during PLRV replication in planta. Western analyses detected P1 and a protein of approximately 25 kDa (P1-C25) that accumulated to readily detectab...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.421
更新日期:1999-01-15 00:00:00
abstract::Robust methods to detect DNA-binding proteins from structures of unknown function are important for structural biology. This paper describes a method for identifying such proteins that (i) have a solvent accessible structural motif necessary for DNA-binding and (ii) a positive electrostatic potential in the region of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh803
更新日期:2004-09-08 00:00:00
abstract::Mammalian mitochondrial ribosomes evolved from bacterial ribosomes by reduction of ribosomal RNAs, increase of ribosomal protein content, and loss of guanine nucleotides. Guanine is the base most sensitive to oxidative damage. By systematically comparing high-quality, small ribosomal subunit RNA sequence alignments an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky762
更新日期:2018-11-16 00:00:00
abstract::Identification of common sub-sequences for a group of functionally related DNA sequences can shed light on the role of such elements in cell-specific gene expression. In the megakaryocytic lineage, no one single unique transcription factor was described as linage specific, raising the possibility that a cluster of gen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl578
更新日期:2006-01-01 00:00:00
abstract::Hg-UMP-containing transcripts made from chick erythroid chromatins with E. coli RNA polymerase hybridize to chick globin cDNA. Contamination with endogenous globin RNA has been largely removed by purification on SH-agarose columns at 55 degrees C. Some endogenous globin mRNA sequences remain, probably as hybrids with ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.4.1237
更新日期:1978-04-01 00:00:00
abstract::Tunable induction of gene expression is an essential tool in biology and biotechnology. In spite of that, current induction systems often exhibit unpredictable behavior and performance shortcomings, including high sensitivity to transactivator dosage and plasmid take-up variation, and excessive consumption of cellular...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky795
更新日期:2018-10-12 00:00:00
abstract::Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alteration...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.3.883
更新日期:1988-02-11 00:00:00
abstract::The small nucleolar RNA U3 is essential for viability in yeast. We have previously shown that U3 can be cross-linked in vivo to the pre-rRNA in the 5' external transcribed spacer (ETS), at +470. This ETS region contains 10 nucleotides of perfect complementarity to U3. In a genetic background where the mutated rDNA is ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.20.4057
更新日期:1994-10-11 00:00:00
abstract::Bacteria core RNA polymerase (RNAP) must associate with a sigma factor to recognize promoter sequences. Promoters recognized by the sigma(54) (or sigma(N)) associated RNA polymerase are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site. Using DNA microarra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1123
更新日期:2010-03-01 00:00:00
abstract::A newly designed cyclic bis-naphthyridine carbamate dimer CMBL4: with a limited conformational flexibility was synthesized and characterized. Absorption spectra revealed that two naphthyridines in CMBL4: were stacked on each other in aqueous solutions. The most efficient binding of CMBL4: to DNA was observed for the s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw672
更新日期:2016-09-06 00:00:00
abstract::Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGC...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.10.4607
更新日期:1988-05-25 00:00:00
abstract::Developmental stage-specific expression of the β-type globin genes is regulated by many cis- and trans-acting components. The adult β-globin gene contains an E-box located 60 bp downstream of the transcription start site that has been shown to bind transcription factor upstream stimulatory factor (USF) and to contribu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku107
更新日期:2014-04-01 00:00:00
abstract::We describe the further development of a widely used package of DNA/protein sequence analysis programs (1). Important revisions have been made based on user experience, and new features, multi-user capability, and a set of large scale homology programs have been added. The programs are very user friendly, economical o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.643
更新日期:1984-01-11 00:00:00
abstract::Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.2.588
更新日期:1998-01-15 00:00:00
abstract::Sequencing of the 3'-untranslated region of the CCT8 gene of the fungal pathogen Candida albicans revealed that the CCT8 coding region overlaps 13 bp with the coding region of the convergently orientated TRP1 gene. The same overlap was found in three strains with different genetic backgrounds. 3'-RACE was used to dete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.22.5061
更新日期:1998-11-15 00:00:00
abstract::Peptides of defined length carrying a diazirine photoaffinity label attached either to the alpha-NH2 group of the N-terminal methionine residue, or to the epsilon-NH2 group of an immediately adjacent lysine residue, were prepared in situ on Escherichia coli ribosomes in the presence of a synthetic mRNA analogue. Pepti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.8.1394
更新日期:1994-04-25 00:00:00
abstract::Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa128
更新日期:2020-05-07 00:00:00
abstract::It is a routine task in metabolic engineering to introduce multicomponent pathways into a heterologous host for production of metabolites. However, this process sometimes may take weeks to months due to the lack of standardized genetic tools. Here, we present a method for the design and construction of biological part...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv464
更新日期:2015-07-27 00:00:00