Abstract:
:Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends on surface-mobile silanes, which are apparently recruited to the condensation site. The yield and species of DNA aggregates can be controlled by selecting the type of functional groups on surfaces, DNA and salt concentrations. For plasmid DNA, the toroidal form can represent >70% of adsorbed structures.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Fang Y,Hoh JHdoi
10.1093/nar/26.2.588subject
Has Abstractpub_date
1998-01-15 00:00:00pages
588-93issue
2eissn
0305-1048issn
1362-4962pii
gkb125journal_volume
26pub_type
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journal_title:Nucleic acids research
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abstract::Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library scree...
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